Figure 6

Time course of HERV-K (HML-2) in de novo HIV-1 infected LC5-RIC cells. The Y-axis shows the x-fold relative expression of HERV-K (HML-2) gag transcripts in infected cells referred to uninfected cells. RNA was isolated from LC5-RIC cells prior to infection and from cells infected with HIV-1IIIB/LAI at day 3, 13 and 22. Relative transcription was quantified according to [85] and normalized to RPII transcript levels. HERV-K (HML-2) transcripts were amplified using gag-specific primers, cloned, sequenced and assigned to proviral loci as described previously [58]. For each HERV-K (HML-2) locus the relative cloning frequency of cDNA is shown as percentage of the total HERV-K (HML-2) transcription level determined by qRT-PCR.