Figure 1

Productivity of different persistently HIV-1 infected human cell lines. (A) Intracellular p24-production in the infected cell lines TH4-7-5, KE37.1-IIIB and LC5-HIV. (B) Extracellular p24-production in the infected cell lines TH4-7-5, KE37.1-IIIB and LC5-HIV. Quantification of p24 antigen in infected cells and in the supernatant was performed as described [82] using a p24 ELISA. (C) HIV-1 transcript levels in the persistently infected cell lines TH4-7-5, KE37.1-IIIB and LC5-HIV. (D) Provirus copy numbers of the infected cell lines TH4-7-5, KE37.1-IIIB and LC5-HIV measured with quantitative PCR. Absolute quantification of HIV proviral copies was performed as described in [82].