The highly polymorphic cyclophilin A-binding loop in HIV-1 capsid modulates viral resistance to MxB
Retrovirology volume 12, Article number: 1 (2015)
The human myxovirus-resistance protein B (MxB, also called Mx2) was recently reported to inhibit HIV-1 infection by impeding the nuclear import and integration of viral DNA. However, it is currently unknown whether there exist MxB-resistant HIV-1 strains in the infected individuals. Answer to this question should address whether MxB exerts an inhibitory pressure on HIV-1 in vivo and whether HIV-1 has evolved to evade MxB inhibition.
We have examined ten transmitted founder (T/F) HIV-1 strains for their sensitivity to MxB inhibition by infecting CD4+ T cell lines SupT1 and PM1 that were stably transduced to express MxB. Two T/F stains, CH040.c and RHPA.c, were found resistant and this resistance phenotype was mapped to the amino acid positions 87 and 208 in viral capsid. The H87Q mutation is located in the cyclophilin A (CypA) binding loop and has a prevalence of 21% in HIV-1 sequences registered in HIV database. This finding prompted us to test other frequent amino acid variants in the CypA-binding region and the results revealed MxB-resistant mutations at amino acid positions 86, 87, 88 and 92 in capsid. All these mutations diminished the interaction of HIV-1 capsid with CypA.
Our results demonstrate the existence of MxB-resistant T/F HIV-1 strains. The high prevalence of MxB-resistant mutations in the CypA-binding loop indicates the significant selective pressure of MxB on HIV-1 replication in vivo especially given that this viral resistance mechanism operates at expense of losing CypA.
Host restriction factors are important components of the antiviral defense. HIV research has led to the discovery of multiple restriction factors that not only inhibit HIV but also a wide range of other viruses. Examples of these restriction factors include APOBEC3G , Trim5α , tetherin [3,4], and SAMHD1 [5,6] (reviewed in ). A recent addition to this important family of proteins is human myxovirus-resistance protein B (MxB, also called Mx2). Despite the well-known antiviral property of MxA , no virus was known to be inhibited by MxB until 2011 when two screen studies showed that vesicular stomatitis virus (VSV), mouse herpesvirus type 68 (MHV-68) and HIV-1 could be restricted by MxB [9,10]. The anti-HIV-1 activity of MxB was further established by three independent studies which reported that MxB impedes HIV-1 DNA nuclear import and/or integration by targeting viral capsid [11-13]. A number of mutations in viral capsid were shown to render HIV-1 resistant to MxB, including N57S, G89V, P90A, N74D and others [12-15]. Furthermore, we identified in the escape viruses a capsid mutation A88T that overcomes MxB inhibition . These results suggested that HIV-1 was able to escape MxB restriction by mutating its capsid sequence. However, the aforementioned capsid mutations are absent in the HIV sequence database likely because they all cause severe defects in virus infectivity. We speculate that, if MxB does exert a strong inhibitory pressure on HIV-1 infection in vivo, then there should exist MxB-resistant HIV-1 strains.
To explore this possibility, we measured the effect of MxB on ten transmitted founder (T/F) HIV-1 strains. The T/F viruses were first produced by transfecting HEK293T cells with their proviral DNA constructs. Virus amounts were then determined by measuring viral reverse transcriptase (RT) activity. Viruses of equal values of RT activity were used to infect a SupT1 cell line that was stably transduced to express MxB under doxycycline induction . Levels of viruses produced by these SupT1 cells were determined by infecting the TZM-bl indicator cells as previously described . Consistent with what our group and others reported [11-13], MxB inhibited the HIV-1NL4–3 virus (a subtype B HIV-1 strain) by 8 to 10 fold (Figure 1A). The ten T/F viruses were inhibited to different degrees, with two strains CH040.c and RHPA.c exhibiting strong resistance to MxB (Figure 1A). We repeated this experiment in another CD4+ T cell line called PM1. Overall, the T/F viruses were less restricted by MxB in PM1 cells than in SupT1 cells (Figure 1B), which is likely attributed to the relatively lower expression levels of the exogenous MxB in PM1 cells (Figure 1C). Importantly, CH040.c and RHPA.c continued to resist MxB inhibition in PM1 cells (Figure 1B). The infectivity of the ten T/F viruses was also measured by infecting the TZM-bl indicator cells (Figure 1D), which showed a wide range of differences between the viruses. It is worth noting that the MxB-resistant strains CH040.c and RHPA.c differed in their infectivity by more than 30 fold. This suggests that the resistance of both viruses to MxB is independent of their infectivity levels.
Given the role of the viral capsid in countering MxB restriction [11-14], we aligned the capsid sequences of CH040.c and RHPA.c with that of NL4-3. Three amino acid positions in the capsid of both CH040.c and RHPA.c differ from those in NL4-3 capsid (Figure 1E). These are H87, H120 and G208 in NL4-3; Q87, N120 and A208 in CH040.c and RHPA.c. We next inserted Q87, N120 and A208 into NL4-3 capsid and generated mutations H87Q, H120N and G208A in order to determine which of these three amino acids lead to MxB resistance. None of these three mutations markedly affected the infectivity of NL4-3 (Figure 1F). When these NL4-3 mutants were tested in MxB-expressing SupT1 cells, H87Q and G208A, but not H120N, were refractory to MxB inhibition (Figure 1G).
H87 is located in the cyclophilin A (CypA)-binding loop of HIV-1 capsid (Figure 2A) . Q87 represents a major variant at this position of capsid in the circulating HIV-1 strains, with 21% representation in the HIV database (Figure 2A) (http://www.hiv.lanl.gov/content/index). This prompted us to test the resistance of other major amino acid variants in the CypA-binding loop, including V86A (4.2%), V86Q (1.0%), H87P (1.1%), A88V (0.1%), I91V (26.9%), A92P (27.4%), M96I (14%) and M96L (6.2%) (Figure 2A). We inserted each of these eight capsid variants into NL4-3. Except for A88V that reduced virus infectivity by 4-fold, the other mutations were well tolerated by HIV-1NL-3 (Figure 2B). We then used these viruses to infect MxB-expressing SupT1 cells and observed that although the I91V, M96I and M96L mutants were as sensitive to MxB inhibition as the wild type NL4-3 (Figure 2C), the mutants V86A, V86Q, H87P, H87Q, A88V and A92P were all resistant to MxB (Figure 2C). Therefore, at least six naturally existing amino acid variants at four positions in the CypA-binding loop render resistance to MxB.
We have previously shown that either knockdown of CypA or cyclosporine A (CSA) treatment to block CypA rescued HIV-1 infection of MxB-expressing SupT1 cells . We therefore asked whether the above capsid variants in the CypA-binding loop modulated HIV-1 susceptibility to MxB by altering the binding of CypA to the viral capsid. To answer this question, we transfected the wild type and mutated HIV-1NL4–3 DNA into HEK293T cells and purified HIV-1 particles by ultracentrifugation through a sucrose gradient . The presence of CypA in HIV-1 particles was examined by Western blotting and the results showed that the wild type NL4-3 and MxB-sensitive mutants I91V, M96I and M96L packaged substantial amounts of CypA as opposed to the MxB-resistant mutants V86A, V86Q, H87P, H87Q, A88V and A92P that packaged little or no CypA (Figure 2D). This data suggests that some HIV-1 strains have evolved to escape MxB inhibition at the expense of losing viral capsid binding to CypA.
Recent studies reported the association of MxB with in vitro assembled HIV-1 capsid [22-24]. Therefore, in addition to modulating the interaction of CypA with HIV-1 capsid, the V86A, V86Q, H87P, H87Q, A88V and A92P mutations may also affect the binding of MxB to the viral capsid. To test this possibility, we infected MxB-expressing SupT1 cells or the control SupT1 cells with wild type HIV-1, or the above six MxB-resistant mutants. The MxB protein was then immunoprecipitated, and the amount of MxB-associated HIV-1 p24/capsid was determined by ELISA. The results revealed that MxB was associated with HIV-1 capsid in the infected SupT1 cells, albeit that both the wild type and mutated viral capsids were associated with MxB to similar degrees (Figure 3A and B). In addition, treatment with CSA did not diminish this interaction (Figure 3C and D). This observation corroborates prior results showing that neither mutations G89V and P90A in the CypA-binding loop nor CSA treatment altered the association of MxB with the in vitro assembled HIV-1 capsid complex [23,24].
In conclusion, we have identified two T/F HIV-1 strains that are resistant to MxB restriction in SupT1 and PM1 cells, and further mapped the resistant mutations to viral capsid. Our data also revealed the high prevalence of MxB-resistant mutations in the CypA-binding loop of the viral capsid. Since these MxB-resistant mutations diminish the association of viral capsid with CypA which acts as a host dependency factor for HIV-1, there is a cost for the virus to adopt this MxB-resistant pathway, which helps to explain why these MxB-resistant mutations have not prevailed the circulating HIV-1 strains. Together with the recent finding that the P207S, G208R and T210K mutations confer resistance to MxB , our data on the G208A mutation reinforces the importance of this region of capsid in modulating HIV-1 sensitivity to MxB. Considering that the A208 residue of capsid has a 35% prevalence rate in the circulating HIV-1 strains (http://www.hiv.lanl.gov/content/index), the G208A variant may serve a distinct mechanism, in addition to the variants in the CypA-binding loop, that HIV-1 employs to escape from MxB restriction in vivo.
Sheehy AM, Gaddis NC, Choi JD, Malim MH. Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein. Nature. 2002;418:646–50.
Stremlau M, Owens CM, Perron MJ, Kiessling M, Autissier P, Sodroski J. The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys. Nature. 2004;427:848–53.
Van Damme N, Goff D, Katsura C, Jorgenson RL, Mitchell R, Johnson MC. The interferon-induced protein BST-2 restricts HIV-1 release and is downregulated from the cell surface by the viral Vpu protein. Cell Host Microbe. 2008;3:245–52.
Neil SJ, Zang T, Bieniasz PD. Tetherin inhibits retrovirus release and is antagonized by HIV-1 Vpu. Nature. 2008;451:425–30.
Laguette N, Sobhian B, Casartelli N, Ringeard M, Chable-Bessia C, Segeral E, et al. SAMHD1 is the dendritic- and myeloid-cell-specific HIV-1 restriction factor counteracted by Vpx. Nature. 2011;474:654–7.
Hrecka K, Hao C, Gierszewska M, Swanson SK, Kesik-Brodacka M, Srivastava S, et al. Vpx relieves inhibition of HIV-1 infection of macrophages mediated by the SAMHD1 protein. Nature. 2011;474:658–61.
Malim MH, Bieniasz PD. HIV restriction factors and mechanisms of evasion. Cold Spring Harb Perspect Med. 2012;2:a006940.
Haller O, Kochs G. Human MxA protein: an interferon-induced dynamin-like GTPase with broad antiviral activity. J Interferon Cytokine Res. 2011;31:79–87.
Schoggins JW, Wilson SJ, Panis M, Murphy MY, Jones CT, Bieniasz P, et al. A diverse range of gene products are effectors of the type I interferon antiviral response. Nature. 2011;472:481–5.
Liu SY, Sanchez DJ, Aliyari R, Lu S, Cheng G. Systematic identification of type I and type II interferon-induced antiviral factors. Proc Natl Acad Sci U S A. 2012;109:4239–44.
Liu Z, Pan Q, Ding S, Qian J, Xu F, Zhou J, et al. The Interferon-Inducible MxB Protein Inhibits HIV-1 Infection. Cell Host Microbe. 2013;14:398–410.
Goujon C, Moncorge O, Bauby H, Doyle T, Ward CC, Schaller T, et al. Human MX2 is an interferon-induced post-entry inhibitor of HIV-1 infection. Nature. 2013;502:559–62.
Kane M, Yadav SS, Bitzegeio J, Kutluay SB, Zang T, Wilson SJ, et al. MX2 is an interferon-induced inhibitor of HIV-1 infection. Nature. 2013;502:563–6.
Busnadiego I, Kane M, Rihn SJ, Preugschas HF, Hughes J, Blanco-Melo D, et al. Host and viral determinants of Mx2 antiretroviral activity. J Virol. 2014;88:7738–52.
Matreyek KA, Wang W, Serrao E, Singh P, Levin HL, Engelman A. Host and viral determinants for MxB restriction of HIV-1 infection. Retrovirology. 2014;11:90.
Keele BF, Giorgi EE, Salazar-Gonzalez JF, Decker JM, Pham KT, Salazar MG, et al. Identification and characterization of transmitted and early founder virus envelopes in primary HIV-1 infection. Proc Natl Acad Sci U S A. 2008;105:7552–7.
Salazar-Gonzalez JF, Salazar MG, Keele BF, Learn GH, Giorgi EE, Li H, et al. Genetic identity, biological phenotype, and evolutionary pathways of transmitted/founder viruses in acute and early HIV-1 infection. J Exp Med. 2009;206:1273–89.
Gamble TR, Vajdos FF, Yoo S, Worthylake DK, Houseweart M, Sundquist WI, et al. Crystal structure of human cyclophilin A bound to the amino-terminal domain of HIV-1 capsid. Cell. 1996;87:1285–94.
Pornillos O, Ganser-Pornillos BK, Yeager M. Atomic-level modelling of the HIV capsid. Nature. 2011;469:424–7.
Zhao G, Perilla JR, Yufenyuy EL, Meng X, Chen B, Ning J, et al. Mature HIV-1 capsid structure by cryo-electron microscopy and all-atom molecular dynamics. Nature. 2013;497:643–6.
Zhang J, Liang C. BST-2 diminishes HIV-1 infectivity. J Virol. 2010;84:12336–43.
Kong J, Xu B, Wei W, Wang X, Xie W, Yu XF. Characterization of the amino-terminal domain of Mx2/MxB-dependent interaction with the HIV-1 capsid. Protein Cell. 2014;5:954–7.
Fricke T, White TE, Schulte B, de Souza Aranha Vieira DA, Dharan A, Campbell EM, et al. MxB binds to the HIV-1 core and prevents the uncoating process of HIV-1. Retrovirology. 2014;11:68.
Fribourgh JL, Nguyen HC, Matreyek KA, Alvarez FJ, Summers BJ, Dewdney TG, et al. Structural Insight into HIV-1 Restriction by MxB. Cell Host Microbe. 2014;16:627–38.
We would like to thank Alexandre Sze for critical reading of the manuscript, Rongtuan Lin for valuable suggestions. HIV-1 transmitted founder viruses were obtained through the NIH AIDS Reagent Program. This research was supported by funding from Canadian Foundation for AIDS Research (CANFAR) and Canadian Institutes of Health Research (CIHR).
The authors declare that they have no competing interests.
ZL (Liu), QP and ZL (Liang) performed the experiments. CL conceived the study. WQ, SC and CL supervised the experimental work, were involved in data analysis and interpretation, and prepared the manuscript. All authors read and approved the final manuscript.
About this article
Cite this article
Liu, Z., Pan, Q., Liang, Z. et al. The highly polymorphic cyclophilin A-binding loop in HIV-1 capsid modulates viral resistance to MxB. Retrovirology 12, 1 (2015). https://doi.org/10.1186/s12977-014-0129-1