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Volume 9 Supplement 2

AIDS Vaccine 2012

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A single dose of SAAVI MVA-C reboosts rhesus macaques after more than 3 years post DNA-MVA prime-boost vaccination

Background

We have previously reported induction of robust immune responses in rhesus macaques following a prime boost immunization with candidate HIV-1 vaccines, SAAVI DNA-C (DNA) and SAAVI MVA-C (MVA). These vaccines are already in clinical evaluation. In the current study, we investigated whether re-boosting these animals with a single MVA inoculation after more than 3 years was sufficient to restore previous magnitudes of HIV-specific immune responses.

Methods

Seven rhesus macaques which had been vaccinated with three doses of DNA vaccine (4mg DNA/dose) and two doses of MVA (109 pfu MVA/dose) in a past study, >3 years previously, were re-boosted with a single dose of MVA. HIV-1-specific responses were quantified in the peripheral blood using an IFN-gamma ELISPOT assay.

Results

A peak magnitude of response (1146±240 sfu/106 PBMC) was reached 1 week after vaccination with the first dose of MVA. The second MVA inoculation did not increase these responses which declined to undetectable levels by 1 year post vaccination. After re-boosting with MVA after 3.5 years post the second MVA, all animals responded, with a peak response (1824±672 sfu/106 PBMC) being reached 1 week after vaccination. Although the mean magnitude of the second peak was not significantly higher than the one seen in the first peak, boosting of responses in 3 of 7 animals with an apparent broadening of the breadth of responses was observed.

Conclusion

These preliminary data suggest a long-term preservation of vaccine memory following a prime-boost vaccination regimen with SAAVI DNA-C and SAAVI MVA-C vaccines.

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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Chege, G., Burgers, W., Muller, T. et al. A single dose of SAAVI MVA-C reboosts rhesus macaques after more than 3 years post DNA-MVA prime-boost vaccination. Retrovirology 9 (Suppl 2), P32 (2012). https://doi.org/10.1186/1742-4690-9-S2-P32

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  • DOI: https://doi.org/10.1186/1742-4690-9-S2-P32

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