- Poster presentation
- Open Access
NMR spectroscopy of HIV-1 gp120 outer domain
© Sastry et al; licensee BioMed Central Ltd. 2012
- Published: 13 September 2012
- Triple Resonance
- Outer Domain
- Backbone Resonance
- Backbone Assignment
- Triple Resonance Experiment
The outer domain (OD) of HIV-1 gp120 has been proposed as a minimal immunogen to elicit broadly neutralizing antibodies. However, OD is heavily glycosylated, contains many flexible regions, and immunization with a number of different OD variants has thus far failed to elicit neutralizing antibodies. An understanding of the conformational space sampled by the OD in its unliganded state, however, may assist in the use of OD as an immunogen.
We developed a method to isotopically enrich glycoproteins using a mammalian expression system that exploits the high level of protein expression obtained from an adenoviral vector, and employed heteronuclear NMR spectroscopy to obtain structural and dynamic information of unliganded OD. Multidimensional NMR experiments were recorded on uniformly labeled 15N/13C OD as well as on samples selectively enriched in 15N-labeled Gly, Ile, Leu and Val. Experiments for backbone assignments were also recorded on an OD sample enriched in 15N/13C for Ile, Leu and Val.
We successfully produced isotopically labeled OD samples, suitable for NMR analysis. We also identified Gly, Ser, Val, Leu and Ile residues using samples selectively enriched in 15N for Gly, Val, Leu and Ile. Standard triple resonance NMR experiments on the isotopically labeled OD were combined with backbone experiments recorded on a second sample – that was selectively enriched in 15N/13C for Ile, Leu and Val – to assign HN, C’, Calpha and N backbone resonances in about 80 of the 220 residues of OD.
We succeeded in assigning ~1/3 of the backbone for unliganded OD with triple resonance experiments. Extension of these assignments with NOESY experiments is now proceeding. Our results indicate that a solution structure of the highly glycosylated HIV-1 gp120 OD is feasible.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.