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Genetic variability and drug resistance mutations in HIV-1 infected individuals on HAART or drug naïve in Limbe, Cameroon
Retrovirology volume 9, Article number: P158 (2012)
Cameroon is a country in West Central Africa with a population of about 20 million inhabitants, with an estimated HIV prevalence of 5.1% in the general population. The HIV epidemic in this region is marked by a broad genetic diversity dominated by Circulating Recombinant Forms (CRFs).
To characterize HIV-1 genotypes circulating in HIV positive individuals in Limbe, Cameroon, we phylogenetically analyzed blood samples from 116 HIV positive patients. Of 116 samples tested, 110 were amplified by nested PCR at the Gag, Pol and Env genes. Sequences obtained were phylogenetically analyzed with reference sequences from the Los Alamos database. The RT region of samples was also amplified to identify mutations that conferred resistance to Reverse Transcriptase inhibitors (RTIs) using the Stanford University Drug resistance database.
Our results revealed a broad genetic diversity, dominated by circulating recombinant forms as follows: CRF02_AG 50.4% (n=54), CRF22_01A1 7.4% (n=8) F2 3.7% (n=4), D 2% (n=2), CRF43_02G 2.8% (n=3), CRF18_CPX 2.8% (n=3) and recombinant forms (RFs) 31.7 %( n=34). Most RFs contained CRF02_AG in one or two HIV loci analyzed. RT sequences of 3 patients on HAART and 15 drug naïve individuals harbored mutations which conferred resistance to RTIs.
CRF02_AG continues to be the most predominant strain in Cameroon, CRF22_01A1 on the increase. Identification of drug resistance strains in drug naïve patients suggest these viruses are being transmitted in the study population and highlights the need of drug resistance testing before start of ART for HIV patients.
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Agyingi, L., Barengolts, D., Mayr, L. et al. Genetic variability and drug resistance mutations in HIV-1 infected individuals on HAART or drug naïve in Limbe, Cameroon. Retrovirology 9, P158 (2012) doi:10.1186/1742-4690-9-S2-P158
- Drug Resistance
- Recombinant Form
- Drug Resistance Mutation
- Drug Resistance Strain
- West Central