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Volume 9 Supplement 2

AIDS Vaccine 2012

  • Poster presentation
  • Open Access

HIV-1 subtype B- and F1-infected subjects display higher cross-clade T-Cell response than subtype C-infected subjects

  • 1,
  • 1,
  • 2,
  • 3,
  • 4,
  • 2,
  • 5 and
  • 1
Retrovirology20129 (Suppl 2) :P150

https://doi.org/10.1186/1742-4690-9-S2-P150

  • Published:

Keywords

  • Peptide
  • Genetic Diversity
  • Infectious Disease
  • Cancer Research
  • Cell Response

Background

The impact of the extensive genetic diversity of the HIV-1 group M isolates and its implications for vaccine design have long been discussed. Studies indicate that Gag and Nef conserved epitopes are commonly recognized and give rise to high cross-clade responses. The aim of this study was to compare T-cell responses to peptide pools derivate from subtype B, C and F1 consensus, among Brazilian subjects infected with those three HIV-1 subtypes.

Methods

The study included 32 subjects infected with HIV-1 subtypes B (n=13), C (n=11) and F1 (n=8). Gag and Nef-specific T cell responses were evaluated by IFN-γ ELISpot assay, using peptide pools based on subtype B, C and F1 Brazilians consensus.

Results

A high cross-clade response between subtypes B and F1 for both Gag and Nef regions was observed among HIV-1 subtype B- and F1-infected subjects. We also found no significant difference in magnitude of responses between subtype B and C consensus peptides in subtype B-infected subjects.In contrast, the magnitude of T cell responses to consensus C peptides in Gag region was significantly higher than to consensus B peptides among HIV-1 subtype C-infected subjects. In Nef, subtype C-infected subjects showed higher T cell responses to C than to F1 consensus peptides. Moreover, subtype F1-infected subjects presented lower responses to subtype C peptides than to subtype F1 and B ones.

Conclusion

Overall, the level of cross-clade response between subtypes B and F1 was higher than between subtype C and B or between subtype C and F1. Our data suggest that significance of the HIV-1 group M genetic diversity for vaccine design may be dependent of the subtypes involved.

Authors’ Affiliations

(1)
Oswaldo Cruz Institute/FIOCRUZ, Rio de Janeiro, Brazil
(2)
Evandro Chagas Clinical Research Institute/FIOCRUZ, Rio de Janeiro, Brazil
(3)
Nova Iguaçu General Hospital, Nova Iguaçu, Brazil
(4)
Evandro Chagas Clinical Research Institute/FIOCRUZ, Rio de Janeiro, Brazil
(5)
Department of Microbiology, Immunology and Parasitology/UFSC, Santa Catarina, Brazil

Copyright

© Côrtes et al; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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