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Evolutionary dynamics of HIV-1 subtype C accessory and regulatory genes in primary infection
Retrovirology volume 9, Article number: P142 (2012)
Studies addressing the dynamics of accessory and regulatory viral gene diversity and selection during early stage of HIV-1 infection are limited but crucial for progress towards vaccine research.
Intra-patient diversity and evolution was assessed during primary HIV-1C infection, viral quasispecies were obtained by single genome amplification (SGA) at multiple sampling time points up to one year post-seroconversion (p/s).
The mean intra-patient diversity was found to be 0.11% (95%CI; 0.02 to 0.20) for vif, 0.23% (95%CI; 0.08 to 0.38) for vpr, 0.35% (95%CI; -0.05 to 0.75) for vpu, 0.18%(95%CI; 0.01 to 0.35 ) for tat exon 1 and 0.30% (95%CI; 0.02 to 0.58) for rev exon 1 during the time period 0 to 90 days p/s. The intra-patient diversity increased gradually in all non-structural genes over the first year of HIV-1 infection, which was evident from the vif mean intra-patient diversity of 0.46% (95%CI; 0.28 to 0.64), vpr 0.44% (95%CI; 0.24 to 0.64), vpu 0.84% (95%CI; 0.55 to 1.13), tat exon 1 0.35% (95%CI; 0.14 to 0.56 ) and 0.42% (95%CI; 0.18 to 0.66) for rev exon 1 during the time period of 181 to 500 days p/s. Statistically significant increases in viral diversity were observed for vif (p=0.013) and vpu (p=0.002). Weak and sporadic associations between levels of viral diversity within the non-structural genes and HIV-1 RNA load during primary infection were found. Positive and negative selection patterns over the first year post-seroconversion were assessed in each of these genes, providing insight into the selection pressures on these genes which are crucial for viral replication in-vivo.
Our study highlights differential diversity and slower diversification across these HIV-1 genes. The most likely cause is different selection pressure imposed by host immune response to the encoded viral gene products that may result in different evolutionary rates.
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Rossenkhan, R., Novitsky, V., Sebunya, T. et al. Evolutionary dynamics of HIV-1 subtype C accessory and regulatory genes in primary infection. Retrovirology 9, P142 (2012) doi:10.1186/1742-4690-9-S2-P142
- Primary Infection
- Viral Diversity
- Sampling Time Point
- Vaccine Research
- Differential Diversity