Volume 9 Supplement 2

AIDS Vaccine 2012

Open Access

Beneficial HLA-mediated viral polymorphisms on the transmitted virus additively influence disease progression in HIV-1, subtype C infection

  • RS Ntale1,
  • DR Chopera1,
  • NK Ngandu1,
  • M Abrahams1,
  • A Debra2,
  • M Mlotswa2,
  • L Werner3,
  • Z Woodman4,
  • K Mlisana3,
  • S Abdool Karim3,
  • CM Gray5,
  • C Williamson1 and
  • CAPRISA 002 AI Study Team1
Retrovirology20129(Suppl 2):O60


Published: 13 September 2012


Transmitted viral factors have been shown to affect disease progression but whether infection with viruses carrying beneficial HLA-mediated escape polymorphisms affects disease progression in HLA-mismatched participants remains controversial.


Gag was sequenced from 56 participants with acute HIV infection from the CAPRISA 002 cohort. For the newly identified mutation, replication fitness of viruses in PBMCs was compared in a subtype C infectious molecular clone. Disease progression was compared in participants infected with viruses carrying polymorphisms associated with beneficial HLA- B*57/58:01 and B7 supertype (B*39:10/81:01) allelic selective pressure in HLA mismatched participants.


In HLA-B*57/58:01-negative participants, 51% (25/49) were infected with viruses carrying at least one of the B*57/58:01 fitness cost-associated escape mutations in ISW9 (A146P:19/49), KF11 (A163G: 10/49) or TW10 (T242N/S: 11/49) epitope. In HLA-B7 negative participants, only 9.4% (3/32) had viruses carrying mutations in the HLA-B7 immunodominant TL9 epitope. Furthermore, we identified in viruses from HLA-B7 participants and included in this analysis a novel mutation in Gag p17, Q65H; found in 9.4% (3/32) of B7 negative participants, and with mutant viruses replicating 11% slower than wild-type in tissue culture. Unlike a previous study in this cohort, infection with viruses carrying mutations in HLA-B*57/58:01 restricted epitopes alone did not impact on viral load setpoint in HLA-mismatched participants, although there was a transient trend towards higher CD4 counts at 3 months post infection (p=0.0551). However, HLA-mismatched participants infected with viruses carrying 3 or more HLA footprints associated with fitness cost in Gag had significantly lower viral load and higher CD4 counts at 3 and 12 months post-infection (p=0.028 and p=0.0264, and p=0.0025 and 0.0689).


These results suggest that multiple mutations generated when viruses are passaged through individuals with beneficial HLAs are needed to attenuate the virus, supporting vaccination methodologies that aim to render the virus less fit.

Authors’ Affiliations

Institute of Infectious Disease and Molecular Medicine, UCT
National Institute for Communicable Diseases
Centre for AIDS Programme Research in South Africa, UKZN
Department of Molecular and Cell Biology, University of Cape Town
Division of Immunology, University of Cape Town


© Ntale et al; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.