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Volume 9 Supplement 2

AIDS Vaccine 2012

Oral presentation | Open | Published:

Immune complexes can dampen inflammatory signaling at the mucosal surface during protective SIV vaccination

Background

The long-term goal for an efficacious HIV vaccine is to provide sterilizing protection from HIV infection. Thus far, this scenario has only been achieved experimentally using live-attenuated SIV vaccines. As such, great interest lies in identifying correlates of protection from a successful host response to pathogenic SIV. To this end, we have used a global genomics approach, tissue analysis, and explant cultures to identify immune complex (IC) signaling as an important component of a protective host response in the female reproductive tract (FRT) of animals vaccinated with the live-attenuated virus known as SIV mac239 ΔNef.

Methods

RNA from cervical tissue was purified for microarray analysis. Significant genes were functionally classified and protein expression determined using single-cell analytical procedures for tissue sections. FRT tissue was removed from healthy, uninfected, adult Rhesus macaques and cervix isolated/dissected into small tissue pieces for ex vivo culturing. WT SIVmac251 32H alone or SIV-specific ICs were added drop-wise to mucosal surfaces of explants and incubated for 24 hr at 37oC / 5% CO2.

Results

A genome-wide transcriptomics analysis revealed selective enrichment of an anti-inflammatory program upon virus exposure in SIVmac239-ΔNef-vaccinated animals, with localized expression of these anti-inflammatory mediators in the mucosal epithelium of the FRT, coinciding with dampened inflammation, limited CD4+ T cell infiltration, and stunted virus replication. Explant cultures derived from the FRT of Rhesus macaques were used as a physiological platform to identify the inhibitory Fc receptor for IgG, FcγRIIB, and carbohydrates in the Fc portion of SIV-specific ICs as centrally important in mediating this anti-inflammatory signaling program in mucosal epithelial cells.

Conclusion

These results highlight an unappreciated, non-neutralizing role for antiviral antibodies at mucosal surfaces and implicates the mucosal epithelial cell as an important host sensor that integrates external signals to elicit a host program that either promotes (inflammatory) or suppresses (anti-inflammatory) immunodeficiency virus infection.

Author information

Correspondence to AJ Smith.

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Keywords

  • Mucosal Surface
  • Rhesus Macaque
  • Explant Culture
  • Female Reproductive Tract
  • Mucosal Epithelial Cell