- Open Access
SAMHD1: a new contributor to HIV-1 restriction in resting CD4+T-cells
Retrovirologyvolume 9, Article number: 88 (2012)
Resting CD4+ T-cells are critical for establishing HIV-1 reservoirs. It has been known for over two decades that resting CD4+ T-cells are refractory to HIV-1 infection, but the underlying mechanisms are not fully understood. Compared with activated CD4+ T-cells that support HIV-1 infection, resting CD4+ T-cells have lower levels of dNTPs, which limit HIV-1 reverse transcription. The dNTPase SAMHD1 has been identified as an HIV-1 restriction factor in non-cycling myeloid cells. Two recent studies revealed that SAMHD1 restricts HIV-1 infection in resting CD4+ T-cells, suggesting a common mechanism of HIV-1 restriction in non-cycling cells that may contribute to viral immunopathogenesis.
HIV-1-induced CD4+ T-cell depletion and establishment of viral reservoir are two hallmarks during AIDS development. Resting CD4+ T-cells are critical for the establishment and maintenance of HIV-1 reservoirs through multifaceted mechanisms . It has been known for over two decades that quiescent/resting CD4+ T-cells are highly refractory to HIV-1 infection [2, 3], particularly to post-entry infection of R5-tropic, but not to X4-tropic viruses since resting CD4+ T-cells express the HIV-1 co-receptor CXCR4. However, the underlying mechanisms of HIV-1 restriction in resting CD4+ T-cells are not fully understood.
Compared with activated CD4+ T-cells that support productive HIV-1 infection, resting CD4+ T-cells have lower levels of dNTPs, which limit efficient HIV-1 reverse transcription and thereby block HIV-1 infection . Addition of exogenous deoxynucleotides as dNTP precursors in quiescent CD4+ T-cells only partially rescues HIV-1 reverse transcription. The dNTP concentrations in quiescent CD4+ T-cells (0.3–5 μM) are approximately 6–10-fold lower relative to activated CD4+ T-cells (3–30 μM) and are around 6–100-fold higher compare with those in non-cycling macrophages (~0.05 μM) [3, 4]. However, the molecular basis of maintaining low dNTP levels in non-cycling cells remained unclear until the recent discovery of a host protein named SAMHD1.
SAMHD1 was initially identified as an HIV-1 restriction factor in non-cycling myeloid cells, including primary monocytes, monocyte-derived dendritic cells and macrophages [5–7]. SAMHD1 has been proposed as a cellular regulator of the intrinsic antiviral response , while its physiological role remains largely unknown. As a dGTP-dependent deoxynucleotide triphosphohydrolase, SAMHD1 hydrolyzes intracellular dNTPs and reduces their concentrations to below the levels required for HIV-1 reverse transcription, thereby blocking viral infection in non-cycling myeloid cells . These studies raised a significant question whether SAMHD1 contributes to HIV-1 restriction in resting CD4+ T-cells. If so, what are the implications of SAMHD1-mediated HIV-1 restriction in CD4+ T-cell depletion, establishment of the viral reservoir, and immune evasion?
New findings and implications
Two recent studies revealed that SAMHD1 restricts HIV-1 infection in resting CD4+ T-cells [9, 10], providing new insights into the mechanisms of HIV-1 restriction in non-cycling cells. SAMHD1-mediated HIV-1 restriction in myeloid cells has been proposed as an immune evasion strategy for HIV-1 to avoid antiviral innate and adaptive immune responses [5–7]. This proposed immune evasion mechanism likely also exists in resting CD4+ T-cells. In addition, HIV-1 restriction mediated by SAMHD1 in resting CD4+ T-cells may contribute to viral immunopathogenesis, such as T-cell depletion and establishment of the viral reservoir and latency (Figure 1).
Both studies by Baldauf et al. and Descours et al. demonstrated that resting CD4+ T-cells isolated from the peripheral blood of healthy donors express high levels of endogenous SAMHD1 protein, which are similar to those detected in primary myeloid cells or the monocytic THP-1 cell line [9, 10]. Baldauf et al. showed that SAMHD1 is highly expressed in explants of human tonsil and likely localizes to non-proliferating macrophages, dendritic cells and CD4+ T-cells , which are the major target cell types when HIV-1 encounters lymphoid or mucosal tissues in vivo. Descours et al. further demonstrated that three circulating CD4+ T-cells subsets, including naïve (CD45RA+, CCR7+), central memory (CD45RA-, CCR7+) and effector memory cells (CD45RA-, CCR7-), express similarly high levels of SAMHD1 . Moreover, circulating CD8+ T-cells and CD19+ B-cells also express SAMHD1 , although the physiologically relevant function of SAMHD1 and its regulation of dNTP levels in these cells remain to be examined. These results expanded our knowledge of SAMHD1 expression profile in major immune cell types that interact with HIV-1.
It has been shown that the lentiviral protein Vpx from HIV-2 and sooty mangabey-lineage SIV (SIVsm) counteracts SAMHD1-mediated HIV-1 restriction in myeloid cells through proteasomal degradation of SAMHD1 [5–7]. Similarly, Vpx from HIV-2 or SIVsm degrades SAMHD1 in resting CD4+ T-cells and efficiently increases single-cycle and replication-competent HIV-1 infection, which is correlated with increased products of full-length viral cDNA and 2-LTR circles in infected cells [9, 10]. However, the degradation of SAMHD1 in resting CD4+ T-cells by treatment of Vpx-containing virus-like particles (VLP-Vpx) derived from SIV appears much less efficient compared with that in primary monocytes and activated CD4+ T-cells . This might be due to distinct efficiencies of nuclear and cytoplasmic exchange among different cell types, given that Vpx-mediated SAMHD1 degradation is dependent on its nuclear export . Furthermore, Vpx increases HIV-1 infection of all resting CD4+ T-cell subsets (naïve, central memory and effector memory cells) by approximately 8- to 15-fold . Importantly, VLP-Vpx treatment neither induces CD4+ T-cell activation and proliferation nor affects HIV-1 infection of activated CD4+ T-cells , indicating that SAMHD1 specifically restricts HIV-1 infection in resting CD4+ T-cells, but not in activated CD4+ T-cells despite comparable expression levels of endogenous SAMHD1 in these cells.
Vpx-mediated SAMHD1 degradation in resting CD4+ T-cells can only enable early steps of HIV-1 life cycle, such as reverse transcription, but not later stages of HIV-1 replication [9, 10]. Descours et al. found that, when SAMHD1 is degraded by Vpx in resting CD4+ T-cells, cytomegalovirus promoter-driven HIV-1 gene expression is significantly enhanced, while HIV-1 LTR promoter-driven viral gene expression remains blocked despite efficient reverse transcription . These results suggest that HIV-1 transcriptional restriction in resting CD4+ T-cells is independent of SAMHD1. By contrast, Baldauf et al. showed that infection of resting CD4+ T-cells with a replication-competent HIV-1 carrying SIV Vpx significantly enhances viral gene expression, which is under the control of the HIV-1 LTR promoter . Notably, Descours et al. used VSV-G-pseudotyped HIV-1 vectors in their infection assays , while Baldauf et al. used a CXCR4-tropic replication-competent HIV-1 . Distinct envelope proteins of HIV-1 used in these studies and/or other different experimental conditions might result in the discrepancy of HIV-1 transcriptional gene expression, which remains to be confirmed. The use of VSV-G-pseudotyped HIV-1 vectors expands the host-cell range to beyond human CD4+ T-cells, suggesting that Descours et al. might examine SAMHD1 function in cell types that are not necessarily relevant to HIV-1 infection. Although Vpx treatment of resting CD4+ T-cells enables early steps of the HIV-1 and HIV-2 life cycle, it cannot enhance the production of HIV-1 p24 or HIV-2 p27 capsid proteins in the supernatants from the infected cells , suggesting that addition mechanisms are involved in HIV-1 and HIV-2 restriction in resting CD4+ T-cells.
Of note, T-cell activation does not affect SAMHD1 expression levels in CD4+ T-cells [9, 10]. Consistent results were observed when CD4+ T-cell activation was achieved either with phytohemagglutinin treatment [9, 10] or through T-cell receptor stimulation with anti-CD3/anti-CD28 in the presence of interleukin-2 . Although activated CD4+ T-cells have 3- to 8-fold higher dATP/dTTP concentrations relative to resting CD4+ T-cells, SAMHD1 expression remains the same in resting and activated CD4+ T-cells . It is likely that intracellular dNTP levels can be significantly increased when activated CD4+ T-cells become dividing cells. How activated CD4+ T-cells upregulate dNTP levels without down-regulating SAMHD1 expression remains to be investigated. Cellular ribonucleotide reductase increases the dNTP pool in cells through the de novo dNTP synthesis pathway. Thus, it is important to analyze expression levels and the activity of ribonucleotide reductases in resting and activated CD4+ T-cells to further understand the mechanisms by which SAMHD1 regulates the dNTP pool and HIV-1 infection efficiency in these cells.
SAMHD1 is primarily a nuclear protein [5, 8] that has a nuclear localization signal in the N-terminus . Using nuclear-cytoplasmic fractionation and three-dimensional reconstructions of confocal images, Baldauf et al. observed that large amounts of SAMHD1 in both the nucleus and cytoplasm of resting CD4+ T-cells, activated CD4+ T-cells, and macrophages , suggesting that SAMHD1 can be a cytoplasm-nucleus shuttling protein that depletes the dNTP pool in the cytoplasm of resting CD4+ T-cells and macrophages where HIV-1 reverse transcription happens. Further microscopy studies of SAMHD1 intracellular trafficking and its interactions with HIV-1 particles in resting CD4+ T-cells and myeloid cells will help dissect the molecular details of SAMHD1-mediated HIV-1 restriction.
Baldauf et al. showed that addition of exogenous deoxynucleotides in resting CD4+ T-cells or infection of resting CD4+ T-cells with Vpx-carrying HIV-1 increases the concentrations of intracellular dATP and dTTP, which correlates with enhanced HIV-1 infection . These results suggest that the intracellular dNTP pools in resting CD4+ T-cells are rate limiting for HIV-1 reverse transcription, and SAMHD1 plays a regulatory role in this process. Furthermore, silencing SAMHD1 expression in post-activated resting CD4+ T-cells using specific siRNA or shRNA significantly increases HIV-1 infection, further suggesting that SAMHD1 suppresses HIV-1 infection in post-activated resting CD4+ T-cells .
SAMHD1 gene mutations are associated with Aicardi-Goutières syndrome (AGS), an inherited autoimmune disease mimicking congenital viral infection with elevated type I interferon production . Berger et al. have reported that, within peripheral blood mononuclear cells (PBMCs) from SAMHD1-deficient AGS patients, CD14+ monocytes are more susceptible to HIV-1 infection compared with cells from healthy donors . Baldauf et al. and Descours et al. further demonstrated that HIV-1 infection of SAMHD1-deficient resting CD4+ T-cells in PBMCs from AGS patients is significantly increased compared with that of SAMHD1-expressing normal cells [9, 10]. Moreover, VLP-Vpx treatment of SAMHD1-deficient resting CD4+ T-cells cannot further enhance HIV-1 infection , indicating that SAMHD1 is necessary for Vpx to relieve HIV-1 restriction in resting CD4+ T-cells. These ex vivo studies using SAMHD1-deficient cells provided further evidence that SAMHD1 functions as an HIV-1 restriction factor.
It is conceivable that SAMHD1-mediated HIV-1 restriction in non-cycling cells may play an important role in viral immunopathogenesis. Doitsh et al. found that abortive HIV-1 reverse transcription in resting tonsil CD4+ T-cells results in cell death through proapoptotic and proinflammatory response, which is triggered by accumulation of incomplete HIV-1 reverse transcripts . However, it is unclear whether and how SAMHD1-mediated restriction of HIV-1 in resting CD4+ T-cells may trigger innate immune recognition of viral cDNA and result in cell death.
Conclusions and future directions
The studies by Baldauf et al. and Descours et al. revealed that SAMHD1 restricts HIV-1 infection in resting CD4+ T-cells by limiting viral cDNA synthesis via depleting the intracellular dNTP pool, at least in part [9, 10]. SAMHD1-mediated HIV-1 restriction in myeloid cells and resting CD4+ T-cells is likely a general mechanism to protect host cells from productive viral infection, which can also be a potential strategy of HIV-1 immune evasion to avoid efficient anti-viral innate immunity .
Because addition of deoxynucleotides in resting CD4+ T-cells only partially restores HIV-1 reverse transcription, other mechanisms may also contribute to HIV-1 restriction imposed by SAMHD1 in non-cycling cells. It is possible that SAMHD1 requires a cellular cofactor in non-cycling cells for its HIV-1 restriction function. On the contrary, it is also plausible that T-cell activation or myeloid cell differentiation diminishes the expression and/or function of a SAMHD1 suppressor, thereby rendering HIV-1 restriction activity of SAMHD1. Further studies are needed to delineate the cellular mechanism by which SAMHD1 inhibits HIV-1 infection in non-cycling cells.
We have reported that polymorphisms of the SAMHD1 gene are not associated with HIV-1 infection and natural control in Europeans and African-Americans . It would be important to examine the role of SAMHD1 in HIV-2 infection given that HIV-1 has not evolved a viral antagonist to SAMHD1. It is also important to further study the effect of Vpx on SIV infection and viral immunopathogenesis using SIV and non-human primate models. SAMHD1 gene knockout mice are currently unavailable. It would be intriguing to examine whether SAMHD1 knockout mice mirror the AGS symptoms in human patients and become more susceptible to the infection with murine retroviruses or other RNA or DNA viruses. The SAMHD1 knockout mice will also be valuable to understand the role SAMHD1 in innate and adaptive immunity.
Post-transcriptional regulation and/or post-translational modifications of SAMHD1 might be important for its function in restricting HIV-1 infection in non-cycling cells. For example, a new study by Welbourn et al. identified naturally occurring splice variants of SAMHD1 in a variety of cell types . These splice variants do not have the dNTPase activity and they are metabolically unstable and therefore can be rapidly degraded in the absence of Vpx , suggesting a post-transcriptional mechanism regulating the hydrolase activity of SAMHD1.
Further elucidating the mechanisms of SAMHD1-mediated HIV-1 restriction in non-cycling myeloid cells and resting CD4+ T-cells may help develop new therapeutic approaches against HIV-1 infection. For instance, one can use pharmacologic induction of low dNTP pools in HIV-1 target cells to inhibit viral infection or to block SAMHD1 activity to elicit protective anti-HIV-1 immune responses. However, these strategies likely will be used in combination with other available anti-HIV-1 interventions and need to be carefully designed and evaluated to avoid potential detrimental effects on the host.
Sterile alpha motif domain- and HD domain-containing protein 1
Human immunodeficiency virus type 1
Human immunodeficiency virus type 2
Simian immunodeficiency virus
Vesicular stomatitis virus G protein
Long terminal repeat
Small interfering RNA
Short hairpin RNA.
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The author thanks members of his laboratory and Dr. Baek Kim (University of Rochester) for helpful discussions. The research in the author’s laboratory is supported by grants (AI098524 and AI102822) from the NIH and by the program of Public Health Preparedness for Infectious Diseases of The Ohio State University. The author apologizes to many whose interesting work could not be cited due to space constraints.
The author declares that he has no competing interests.
LW conceived the topic and wrote the manuscript.
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