- Meeting abstract
- Open Access
Epidemiological determinants and PCR results in Central African inhabitants with a new and frequent HTLV indeterminate Western Blot pattern exhibiting mostly p28, p32, p36, and a shifted GD21
© Filippone et al; licensee BioMed Central Ltd. 2011
- Published: 6 June 2011
- Western Blot
- Infectious Disease
- Cancer Research
- Primer Pair
- Plasma Sample
HTLV indeterminate WB patterns are frequently observed in plasma/serum samples from persons living in intertropical areas.
In the framework of ongoing projets on HTLV-1/2 and related viruses in central Africa, we systematically analysed by WB, plasma from villagers living in south Cameroun. The studied group included 2155 individuals (mean age 44, range 2-90, 982 women/1173 men), either Bantous (1258) or Pygmies (897). All plasma samples were tested by WB (HTLV 2-4 MPD) with interpretation done according to manufacturer instructions. Only clear bands were considered as positive/informative. DNA extracted from buffy-coat were subjected to PCR using several primer pairs known to detect HTLV-1/2/3/4. Positive PCR bands were sequenced.
Among the 2155 plasma samples, 48 were HTLV-1, 20 HTLV-2, and 134 HTLV. Furthermore, 955 were indeterminate including 100 HGIP (HTLV-I Gag-indeterminate pattern) , and 57 with a peculiar pattern exhibiting mostly p28, p32, p36, and a shifted GD21. The other samples were either WB negative (998) or exhibited mostly faint or unique p19 or p24 bands. Most HTLV-1 samples and some HTLV were found PCR positive. In contrast, all the others (HTLV-2, HGIP, new WB pattern and other indeterminate) were found PCR negative except in one case of a HTLV-3 infection . Epidemiological determinants of the persons with this new pattern were different from those with HTLV-1.
Search for the origin of this frequent new WB is ongoing with special insights concerning cross-reactivities with parasitic antigens as suggested for the HGIP pattern .
- Mauclère P, Le Hesran JY, Mahieux R, Salla R, Mfoupouendoun J, Abada ET, Millan J, de Thé G, Gessain A: Demographic, ethnic, and geographical differences between human T cell lymphotropic virus (HTLV) type I-seropositive carriers and persons with HTLV-I Gag-indeterminate Western blots in Central Africa. J Infect Dis. 1997, 176 (2): 505-509.View ArticlePubMedGoogle Scholar
- Calattini S, Betsem E, Bassot S, Chevalier SA, Mahieux R, Froment A, Gessain A: New strain of human T lymphotropic virus (HTLV) type 3 in a Pygmy from Cameroon with peculiar HTLV serologic results. J Infect Dis. 2009, 199 (4): 561-564. 10.1086/596206.View ArticlePubMedGoogle Scholar
- Mahieux R, Horal P, Mauclère P, Mercereau-Puijalon O, Guillotte M, Meertens L, Murphy E, Gessain A: Human T-cell lymphotropic virus type 1 gag indeterminate western blot patterns in Central Africa: relationship to Plasmodium falciparum infection. J Clin Microbiol. 2000, 38 (11): 4049-4057.PubMed CentralPubMedGoogle Scholar
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