Volume 8 Supplement 1

15th International Conference on Human Retroviruses: HTLV and Related Viruses

Open Access

Transformation of human embryonic stem cells by HTLV-1 Tax

  • Linda Zane1,
  • Junichiro Yasunaga1,
  • Takao Kinjo1,
  • Gustavo Brito de Melo1 and
  • Kuan-Teh Jeang1Email author
Retrovirology20118(Suppl 1):A164

https://doi.org/10.1186/1742-4690-8-S1-A164

Published: 6 June 2011

HTLV-1 encodes a viral oncoprotein Tax which has been shown to transform primary rodent cells. Tax can also immortalize human primary T lymphocytes. However, to date transformation by Tax of human cells has been unsuccessful [1, 2]. Based on the cancer stem cells (CSCs) hypothesis that tumor growth is caused by the proliferation of only a small number of CSCs, we reasoned that ATL cells may arise from the infection by HTLV-1 of not mature CD4+ T cells (as currently thought) but by viral infection of more rare hematopoietic precursor cells. Thus, Tax may be able to transform early undifferentiated human cells, but not later differentiated cells. We asked whether Tax could transform human Embryonic Stem Cells (ESCs) vs. human skin fibroblast BJ cells. Undifferentiated ESCs were transfected with Tax, and these cells were injected into immunodeficient NOD-SCID/IL2γR null (NOG) mice. We assessed tumor incidence after injection and found that Tax enhanced the tumorigenesis of ESCs (p=0.012, Gehan-Breslow-Wilcoxon test). By contrast, our transformation studies using Tax in differentiated human BJ cells have been consistently unsuccessful. Based on these results and others to be presented, we conclude that HTLV-1 Tax is more capable of inducing tumorigenesis of undifferentiated than differentiated human cells.

Authors’ Affiliations

(1)
National Institute of Allergy and Infectious Diseases, NIH

References

  1. Higuchi M, Fujii M: Distinct functions of HTLV-1 Tax1 from HTLV-2 Tax2 contribute to key roles in viral pathogenesis. Retrovirology. 2009, 6: 117-10.1186/1742-4690-6-117.PubMed CentralView ArticlePubMedGoogle Scholar
  2. Matsuoka M, Jeang KT: Human T-cell leukemia virus type 1 (HTLV-1) and leukemic transformation: viral infectivity, Tax, HBZ and therapy. Oncogene. 2011, Epub a head of printGoogle Scholar

Copyright

© Zane et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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