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Open Access

Susceptibility of HBZ and Tax expressing primary HTLV-1+ T cell clones to CTL lysis

  • Aileen G Rowan1Email author,
  • Becca Asquith1,
  • Graham P Taylor2 and
  • Charles R M Bangham1
Retrovirology20118(Suppl 1):A105

Published: 6 June 2011


Infected IndividualViral Gene ExpressionAntisense StrandProviral LoadHigh Avidity

Host cytotoxic lymphocyte (CTL) responses play an essential role in limiting clonal expansion of infected CD4+ T cells in HTLV-1+ individuals. We have reported that individuals who possess HLA class 1 alleles that strongly bind peptides from HTLV-1 basic leucine zipper protein (HBZ) have a lower proviral load and are less likely to develop HAM/TSP. In general, peptides from HBZ bind weakly to MHCI, and we detected low frequencies of HBZ-specific CD8+ T cells in ≈25% of infected individuals. Despite its poor immunogenicity, high avidity CTL lines can be generated in vitro which efficiently lyse HBZ-presenting cells [1].

HBZ mRNA, encoded on the antisense strand of the viral genome, can induce proliferation of T cells, and once translated it can inhibit expression of Tax. HBZ mRNA is expressed in vivo in the majority of infected individuals, however, HBZ protein expression levels are extremely low in PBMCs ex vivo. To investigate the relationship between Tax and HBZ expression in naturally infected CD4+ T cells at the single cell level, we cloned T cells from individuals with HAM/TSP. We detected HBZ mRNA in all clones tested. In contrast, a subset of infected clones stably expressed Tax protein. Thus, individual clones may have a unique viral gene expression profile, with differing susceptibility to lysis by HTLV-1 specific CTL. We are testing the hypothesis that targeting of Tax– clones by HBZ-specific CTL eliminates clones which otherwise escape lysis by abundant Tax-specific CTL.

Authors’ Affiliations

Department of Immunology, Imperial College London, UK
Section of Infectious Diseases, Imperial College London, UK


  1. Suemori K, Fujiwara H, Ochi T, Ogawa T, Matsuoka M, Matsumoto T, Mesnard J-M, Yasukawa M: HBZ is an immunogenic protein, but not a target antigen for human T-cell leukemia virus type 1-specific cytotoxic T lymphocytes. J Gen Virol. 2009, 90: 1806-1811. 10.1099/vir.0.010199-0.View ArticlePubMedGoogle Scholar


© Rowan et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.