HIV-1 produced in PBMCs is transmitted by CLEC-2. (A) Stocks of HIV-1 NL4-3 were generated in 293T cells (left panel) and in PBMCs (right panel) and used for transmission and direct infection, employing the indicated cell lines, as described for figure 4. The results of representative experiments performed in triplicate are shown, error bars indicate SD. Similar results were obtained in two independent experiments (B) The experiment was carried out as described in (A) but transmission of HIV-1 SF33 generated in PBMC was analyzed. The results of a representative experiment performed in triplicate are presented and were confirmed in a separate experiment; error bars indicate SD. C.p.s.: counts per second, SD: standard deviation.