HIV-1 assembly in macrophages
Retrovirology volume 7, Article number: 29 (2010)
The molecular mechanisms involved in the assembly of newly synthesized Human Immunodeficiency Virus (HIV) particles are poorly understood. Most of the work on HIV-1 assembly has been performed in T cells in which viral particle budding and assembly take place at the plasma membrane. In contrast, few studies have been performed on macrophages, the other major target of HIV-1. Infected macrophages represent a viral reservoir and probably play a key role in HIV-1 physiopathology. Indeed macrophages retain infectious particles for long periods of time, keeping them protected from anti-viral immune response or drug treatments. Here, we present an overview of what is known about HIV-1 assembly in macrophages as compared to T lymphocytes or cell lines.
Early electron microscopy studies suggested that viral assembly takes place at the limiting membrane of an intracellular compartment in macrophages and not at the plasma membrane as in T cells. This was first considered as a late endosomal compartment in which viral budding seems to be similar to the process of vesicle release into multi-vesicular bodies. This view was notably supported by a large body of evidence involving the ESCRT (Endosomal Sorting Complex Required for Transport) machinery in HIV-1 budding, the observation of viral budding profiles in such compartments by immuno-electron microscopy, and the presence of late endosomal markers associated with macrophage-derived virions. However, this model needs to be revisited as recent data indicate that the viral compartment has a neutral pH and can be connected to the plasma membrane via very thin micro-channels. To date, the exact nature and biogenesis of the HIV assembly compartment in macrophages remains elusive. Many cellular proteins potentially involved in the late phases of HIV-1 cycle have been identified; and, recently, the list has grown rapidly with the publication of four independent genome-wide screens. However, their respective roles in infected cells and especially in macrophages remain to be characterized. In summary, the complete process of HIV-1 assembly is still poorly understood and will undoubtedly benefit from the ongoing explosion of new imaging techniques allowing better time-lapse and quantitative studies.
Role of monocytes/macrophages in HIV-1 physiopathology
Rapidly after the discovery of HIV-1, it was established that HIV-1 has two major targets in vivo; T lymphocytes, which have been extensively studied, and macrophages. While the viral replication cycle is usually rapid and cytopathic in T cells, infected macrophages survive for months in vitro and in vivo, and accumulate large vacuoles containing infectious viral particles [1–3]. HIV-1 enters the Central Nervous System (CNS) soon after peripheral infection of circulating T cells and monocytes and probably penetrates the CNS at various times during infection, see review . Immunohistochemistry and in situ hybridization studies have demonstrated that, in the CNS, perivascular macrophages and microglia are the most productively HIV-infected cells and are likely to mediate CNS dysfunctions observed in individuals infected with HIV-1 . Intracellular location has long been considered to provide a privileged niche, protecting the virus from the immune system as well as from the action of antiviral drugs. Thus, HIV-1 can persist in a protected brain reservoir made of infected monocytes/macrophages despite anti-retroviral therapy. Therefore upon arrest of highly active antiretroviral therapy, macrophages but also blood monocytes  may contribute to the spread of HIV-1 and the rapid reconstitution of high viral loads.
Macrophages differentiate from monocytes and represent a very diverse population of phagocytes, present in many tissues and involved in various functions (from bone remodeling to muscle regeneration, see review ) acting in both innate and adaptive immunity. Their first function is to phagocytose cellular debris and pathogens either as stationary or mobile cells. Therefore, they possess a very active endo-lysosomal system, the activity and rapidity of which may have been underestimated. Looking at the ultra-structural level at human macrophages, one is struck by the richness of the endo-lysosomal network and the paucity of intermediate compartments suggesting that internalized materials are very rapidly targeted to lysosomes .
Scope of the present review
Despite the importance of macrophages for the physiopathology of AIDS, and the initial interest after their identification as the second main target of the virus in vivo, very little is known about the HIV-1 cycle in macrophages. Most studies have been performed in non-macrophage cell lines. and it is unclear whether such results hold true in macrophages. Here, we will review the HIV assembly process within infected primary macrophages, i.e. most commonly, monocyte-derived macrophages.
Current view(s) of HIV-1 assembly
Coordinating viral assembly
In this section, we focus on the late events of viral replication in macrophages. Currently, it remains unclear how the various components of the viral particle are targeted to the assembly compartment of which the exact nature and localization remain elusive (see Figure 1 for a summary). Early studies showed that infected macrophages tend to accumulate intracellular vacuoles that contain numerous viral particles [1, 2, 8]. Since budding events have been observed at the limiting membranes of these vacuoles, [9, 10], they are generally considered as the site of HIV-1 assembly in macrophages. We will refer to these vacuoles as the viral assembly compartment in the present review.
The trafficking of viral components to the assembly site as well as their subsequent assembly and release in the form of an infectious particle are coordinated and regulated through interactions between viral structural proteins and cellular factors. The product of the gag gene has long been recognized as the main conductor of HIV-1 assembly since its expression alone gives rise to virus-like particles having the same spherical shell structure as immature viral particles [11, 12]. The current view of HIV-1 assembly in T cells has been recently reviewed [13, 14], and we will only give here a brief overview of the process.
Gag is composed of three polypeptides-- the matrix, the capsid, the nucleocapsid; and three smaller peptides that function together to coordinate membrane binding and Gag-Gag lattice interactions in immature virions . One of the three peptides is called p6 or the "late domain" because it is required for virus budding and release . The Gag precursor is synthesized in the cytosol and co-translationally myristoylated at its N-terminus, which is required for stable membrane association. It is then targeted to the cytoplasmic leaflet of membranes through mechanisms that are not fully understood. There, Gag multimerizes into microdomains, which in turn stabilize its membrane association .
Gag can be found in the cytosol as small oligomers detected by immuno-EM , but it is not known whether Gag oligomerization is a prerequisite for the spherical Gag lattice formation. Similarly, it remains unclear whether the transport of the precursor relies on free cytoplasmic diffusion or if it requires trafficking along the cytoskeleton. It has also been suggested that RNA binding to Gag could play a role in the assembly process by providing a scaffold to stabilize intermolecular Gag interactions [19, 20]. Where and when the interaction between Gag and viral RNA occurs is still debated, but the trafficking of genomic RNA may influence Gag cytosolic fate [21–24]. Of note, the majority of data concerning intracellular Gag trafficking was obtained from immortalized cell lines and does not necessarily reflect the situation in infected primary macrophages.
Host factors involved in assembly
Among the numerous cellular factors reported to be involved in HIV-1 assembly and budding, the ESCRT cellular machinery (Endosomal Sorting Complex Required for Transport) is recruited by the p6 domain and plays a key role in the formation and release of new particles. This complex has drawn a lot of attention, and much progress has been made in the last few years in understanding its way of functioning in three important processes: formation of intraluminal vesicles in multi-vesicular bodies (MVBs), HIV-1 budding and fission from membranes, and more recently in fission of the midbody during cytokinesis. The three processes have in common the need for severing a thin membrane to allow vesicles, nascent viral particles, or cells to be released. Since this large body of work has not been reproduced in macrophages and because the mechanisms involved have been thoroughly reviewed [13, 15, 25], they will not be discussed here.
Additionally, Vpu, one of the accessory proteins of HIV-1, also plays a crucial role in the terminal step of particle release (see ). Indeed, Vpu has been recently shown to counteract the activity of a restriction factor named tetherin/BST-2/CD317 [26–30]. In the absence of Vpu, viral particles bud from the plasma membrane of T cells but cannot detach due to the presence of tetherin. The action of Vpu in T cells may rely on the down-regulation of BST-2 at the cell surface through both relocalization and degradation of this factor [31–33]. The molecular mechanism involved in this tetherin-mediated retention remains unknown as well as the exact role of Vpu in different cell types, especially in macrophages .
Other cellular players
In addition to the ESCRT machinery many cellular proteins are thought to be recruited or affected for efficient viral assembly and release [15, 34]. Only a few of those factors have been characterized in macrophages. One of them is a cholesterol transporter named ABCA1, which when bound to Nef could result in the impairment of cholesterol efflux in infected macrophages . This may be related to the requirement of cholesterol in the viral envelope for better infectivity. Another factor reported to be essential for both productive infection of macrophages and the infectivity of released virions is Annexin2 which binds to Gag at the limiting membrane of the viral assembly compartment . Annexin 2 seems to be involved in many functions including membrane trafficking and endosome formation, and its intracellular distribution depends on cholesterol . Since Annexin2 is not expressed by lymphocytes, its expression in macrophages may contribute to the particular localization of their viral assembly site.
Studies performed with cells other than macrophages have revealed many proteins involved in the trafficking of Gag or Env towards the assembly site or its regulation, such as Clathrin adaptors AP-1, AP-2 and AP-3 [38–44], clathrin-binding factors GGAs and their regulator Arf  and TIP47, which could simultaneously bind to Env and Gag . The microtubule network could play a role via the inducible host factor SOCS1 in the intracellular trafficking of Gag [47–49], as well as the kinesin KIF4 which binds to Gag and is required for viral assembly [50, 51]. Moreover, a thorough proteomic analysis of purified virions produced by HIV-1-infected macrophages showed the presence of numerous of these host proteins .
The above list of cellular proteins involved is far from exhaustive. Recently, siRNA-based genome-wide screens by 4 independent teams have identified cellular proteins potentially involved at various stages of the viral cycle [53–56]. These studies have produced large numbers of candidates of which very few overlap. This may reflect, in part, differences in the experimental set up used for each of these screens which used different HIV-1 isolates and cell lines (HEK293T or HeLa cells and Jurkat cells; see meta-analysis  and comment ). While many proteins have been proposed to play roles in the HIV-1 assembly process, their respective contributions and the temporal order of the events are far from established.
Approaching HIV-1 assembly in primary macrophages
Many studies have been based on immuno-fluorescent staining of viral proteins such as Gag in infected macrophages. In fact, Gag has multiple localizations in infected cells (see an example in Figure 2, typical of day 7 post-infection). Gag goes from a diffuse cytosolic pattern to small dots in the periphery to large intracellular compartments. Moreover, the Gag staining pattern evolves with time post-infection. Two additional reasons render the interpretation of these staining even more complex:
i) The poor resolution of the epifluorescent microscopy technique does not allow one to distinguish mature or nascent viral particles from Gag aggregates. Note that the diameter of an immature viral particle is in the range of 100 to 200 nm (mean 129 nm) , which is below the resolution of epifluorescent microscopes.
ii) It is impossible to distinguish incoming virions, which may fuse or be internalized, from nascent viral particles eventually being secreted. Similarly, there is no way to know whether dots observed by immunofluorescence represent infectious or non-infectious particles. Finally, we do not know if all the synthesized Gag precursor has a homogeneous behavior or if several populations of Gag precursor exist with distinct fate and function. This idea is supported by Gousset et al. showing that only part of Gag was redistributed in infected macrophages towards the synapse formed with non-infected T cells .
Some of these problems can be, in theory, circumvented by ultrastructural approaches. So far, only Immuno-electron microscopy (Immuno-EM) allows one to distinguish viral particles, from viral buds, and from non-assembled Gag. However, this technique remains tedious, difficult to master, and only works with very few antibodies on fixed samples.
How ultrastructural studies have shaped our representation of HIV-1 assembly in macrophages
EM studies have greatly influenced our view of the viral cycle in macrophages. Early work revealed the existence of large intracellular vacuoles in which viral particles tend to accumulate. Raposo et al. showed by immuno-EM that these vacuoles contained not only virions, but also endosomal markers such as MHC II and CD63. Based on EM profiles they also proposed that viral budding takes place at the limiting membrane of the compartments, and that fusion of these compartments can occur at the plasma membrane leading to the release of their contents; HIV-1 particles and exosomes . Pelchen-Matthews et al. confirmed these results and provided additional biochemical evidence that viral particles originate from late endocytic compartments and carry markers from these compartments [10, 61].
To our knowledge, only one team observed by Immuno-EM some ESCRT-related specific staining at the limiting membrane of these compartments . However, these ESCRT-components were also present elsewhere in the cell and did not appear to be relocated to the site of viral assembly upon HIV infection . In our preparations of macrophages, Alix and CHMP4 were present mainly in virions, but also at the limiting membrane of the viral assembly compartment (Figure 3). However, we did not succeed in finding other ESCRT-specific antibodies effective for immuno-EM despite testing a large collection. This difficulty may reflect the tightness of the ESCRT multi-protein complex. This also points to the limitations of the immuno-EM studies for which few antibodies can be used on ultrathin sections. Nevertheless, it is now well-accepted that the ESCRT machinery is recruited by HIV-1 in macrophages as well as in T cells at their respective locations for HIV-1 assembly, either inside the cell or at the plasma membrane.
Nature of the viral assembly compartment in macrophages
Where does viral assembly take place in infected macrophages?
Initial studies suggested the existence of an intracellular compartment specialized in the assembly and storage of viral particles. Ultrastructural studies revealed budding profiles at the limiting membrane of internal compartments  in a process and topology similar to the biogenesis of internal vesicles or exosomes in MVBs, which are late endosomes . Similar profiles were reported later [10, 64, 65]. Proteomic analysis of the host cell proteins incorporated into highly purified virions produced by macrophages revealed the presence of many late endosomal proteins such as MHC II, CD63, and tetraspanins  which is in agreement with immuno-EM studies [9, 10, 61]. Moreover, such virions and macrophage-derived exosomes had similar protein compositions . Using recombinant viruses in which a tetracysteine tag was introduced at the C-terminus of the matrix domain of Gag, it has been possible to visualize Gag trafficking in living macrophages. Accumulations of Gag were observed both at the plasma membrane and in internal compartments carrying late endosome/MVB markers .
Other arguments supporting the idea that productive intracellular assembly takes place in MVB-like compartments are weak as they come from studies performed in cell lines such as HeLa, HEK 293 T, or COS cells [67–69]. Viral budding was observed in MVBs from such cells , while Gag was found to be transported to CD63+ MVBs in an AP3-dependent manner . It has been also suggested that Gag transiently traffics through MVB-like compartment to recruit the ESCRT machinery before reaching the plasma membrane in these cell lines . Recently, Joshi et al. used a HIV-1 carrying a Gag-matrix mutant (29/31KE) which localizes to MVBs in all cell types, thus showing that efficient intracellular assembly and release of viral particles occurred not only in macrophages but also in T cells . This study therefore establishes that endosomal compartments can serve as productive sites for HIV-1 assembly in both T cells and macrophages.
A characteristic of the endocytic pathway is its progressive acidification which allows the activation of degradative enzymes. Endosomes would therefore constitute a hostile environment for HIV-1 which is a fragile virus sensitive to low pH and proteases . However, HIV-1 remains infectious in macrophages, even after residing in macrophages for long periods of time . Simultaneous identification by immuno-EM of viral assembly compartments and estimation of their pH were carried out on infected macrophages . While the extended network of lysosomes present in infected macrophages was correctly acidified, viral compartments were not. Endosomal acidification is required for maturation along the endocytic pathway and fusion with lysosomes. Therefore, HIV-1 may have evolved a strategy for survival in macrophages.
It has been proposed that intracellular virions observed in HIV-1-infected macrophages represent endocytosed particles produced by neighboring cells . Several arguments can be put forward to rule out this hypothesis: 1) Immuno-EM profiles obtained by several teams show viral particles at various stages of budding at the limiting membrane of the compartment [2, 9, 10, 65]. Moreover, the viral particles seen in these compartments were often immature virions, as judged by their electron lucent material at the core and electron dense material at the periphery (see Figure 1 a schematic representation). 2) Shortly after exposure of macrophages to HIV-1, most virions are found in macropinosomes or in acidic endosomes and are subsequently degraded [65, 75]. 3) In all the studies mentioned, the HIV-1 strains used expressed Vpu, which promotes virus release but also inhibits virus uptake by endocytosis [28, 76]. Taken together, this strongly suggests that the majority of viral particles detected in intracellular compartments of HIV-1 infected macrophages have been de novo produced rather than recently endocytosed.
A compartment connected to the plasma membrane
Despite the numerous evidence showing that HIV-1 assembly occurs in macrophages in MVB-related compartments, recent studies have challenged this view. They were based on the usage of the ruthenium red (RR), which is a membrane-impermeant dye added during the fixation of infected macrophages and before their analysis by electron microscopy. Deneka et al. suggested that at least some of the virus-positive, "intracellular" structures in macrophages were actually connected to the plasma membrane via very thin microchannels allowing access of the RR dye . Another team achieved similar results , and both concluded that the viral assembly compartment originates from the plasma membrane in infected macrophages. We also observed in our macrophage preparations that some viral compartments were RR+; however, 80% of them remained negative (Figure 4 and ). Interestingly, we frequently noticed in the vicinity of the viral compartments numerous electron-dense lipid droplets that were heavily stained by the RR dye (Figure 4A, see white asterisks) in agreement with the known capacity of RR to bind lipids and suggesting their connection to the extracellular space. As previously reported for other cell types [78, 79], our pictures on Figure 4 reveal however the presence of electron-dense RR+ areas in the cytoplasm and mitochondria near lipid droplets, and thus indicates that the RR dye is not totally membrane-impermeant in macrophages.
A very recent study based on ion-abrasion scanning electron microscopy indicates that HIV-1-infected macrophages possess an extensive network of tubules occasionally connecting virus containing compartments with the cell surface . These virion-containing tubules have a diameter of 150-200 nm and thus may differ from the narrow (< 20 nm) virion-free microchannels mentioned above. Future work will aim at confirming and quantifying the presence of these microchannels or tubules using alternative techniques.
It is currently not known whether these connections to the plasma membrane are transient or permanent. However, they may account for the lack of acidification of the viral compartment mentioned above. They could also occur as an early event during the establishment of the intracellular vacuole; or on the contrary, they may precede an exocytosis process of the viral particles, although the diameter of the microchannels appears too small to accommodate virus trafficking (around 20 nm, ).
Despite hundreds of EM profiles of HIV-1-infected macrophages analyzed, we never saw any budding event taking place at the plasma membrane like we observed in T cells (M. J. and P. B., unpublished observations). Importantly, three studies on macrophages showed that the viral compartments were accessible to Transferrin, but not to BSA-gold or immunoglobulin-coated gold beads added to the extracellular medium [2, 64, 65], supporting the concept of a compartment separated from the endocytic pathway but capable of exchanges with the recycling compartment. Alternatively, Transferrin access may be due to the microchannel connections to the plasma membrane.
Altogether it remains unclear whether the viral compartments observed in HIV-infected macrophages correspond to invaginations of the plasma membrane. We favor the notion of an intracellular compartment separated from the endocytic pathway, possessing a neutral pH and transiently connected via microchannels to the plasma membrane. However, more work is needed to resolve the nature of the viral compartment in macrophages.
Composition of the compartment
The limiting membrane of the compartment where viral budding takes place will eventually wrap the nascent viral particle. Therefore the lipid and protein composition of the viral membrane may reflect the origin of the assembly compartment (see ). The HIV-1 membrane is enriched in cholesterol, GM1 and tetraspanins, supporting the idea that HIV-1 budding could take place on lipid raft-like membranes. However, several proteins known to be normally associated with rafts like CD14 and CD45 are not found in viral envelope, whereas some proteins present in HIV-1 envelope appear excluded from lipid-rafts .
Tetraspanins such as CD9, CD53, CD81 and CD82 were enriched both in the compartment and in the viral membrane [10, 61, 82]. Although CD63 was specifically associated with HIV-1 assembly compartments in macrophages, it was dispensable for the production of infectious virus . However, opposite results were obtained also in macrophages . Learning more about the function of CD63, which remains elusive, will probably help to solve this discrepancy.
The limiting membrane of the viral compartment often appears to contain molecular coats (see  and Figure 3B') of which, the composition remains elusive. These coats are reminiscent of flat clathrin lattices found in MVBs  but they appear less flat, do not contain clathrin and are also observable on the microchannels connecting the compartments to the plasma membrane .
The "sponge-like structures"
Deneka et al. reported the frequent presence of "sponge-like" structures in the immediate vicinity of viral assembly compartments in infected macrophages . These structures are very rich in highly interconnected membranes and accessible to the RR dye. We also observed in our macrophage preparations such RR+ structures (Figure 4C), of which the nature and function remain so far unknown. As previously noticed , their morphology appears similar to structures observed in primary macrophages that have been exposed to aggregated low-density lipoproteins and that are also efficiently stained by RR (see ).
HIV-1 is known to wrap into cholesterol-rich membranes that are required for viral production and infectivity. Since cholesterol efflux is inhibited in HIV-1-infected macrophages through a Nef-dependent mechanism , this accumulation of lipids may contribute to the appearance of the sponge-like structures. However, Nef does not promote the intracellular accumulation of viral particles in macrophages  and is dispensable for effective HIV-1 replication in macrophages [86, 87]. Future work will elucidate the connection between lipid homeostasis, Nef and the assembly process in macrophages.
Features of the HIV-1 cycle in macrophages still need to be better established but appear to be different at many steps from what is known during infection of CD4+ T cells (see accompanying reviews in the present issue of Retrovirology). Studying HIV-1 assembly in primary macrophages remains a difficult task for several reasons: Macrophages are refractory to most transfection procedures, and their very strong adherence to plastic culture dishes makes them very difficult to detach. They are terminally differentiated and thus cannot be expanded. Upon HIV infection, macrophages tend to form large syncitia and display quite a bit of donor-to-donor variability. There is a crucial need for quantitative studies that cannot be performed using conventional techniques. Several recent studies have been carried out using time-lapse based technologies, with the help of recombinant HIV-1 viruses engineered to produce fluorescent particles [20, 88, 89]. Recombinant viral particles can be tracked by spinning-disk confocal or TIRF microscopy. Such studies have been performed essentially with cell lines, but also in primary T cells. So far they have shed light and brought information regarding the dynamics of viral transmission between T cells, or between macrophages and T cells, and on viral entry in HeLa cells.
Despite the recent advances, many features of the HIV-1 assembly process in macrophages remain to be elucidated. Beside the exact nature and biogenesis of the viral assembly compartment, several questions have to be addressed. Among them: what are the stimuli and processes leading to the release of viral particles by infected macrophages? Is there a way of controlling this release, for example through a targeted delivery of the viral particles at the virological synapse? Given that the molecular mechanisms involved in exosome secretion are just beginning to be approached , a lot remains to be done. The impact of viral secretion by macrophages on cell-to-cell transmission could be very important from a physiopathological point of view, especially when highly-active anti-retroviral therapies are stopped. Virological synapses allow HIV-1 trans-infection from infected to uninfected macrophages . Rapid transfer of HIV-1 particles from macrophages to autologous CD4+ T cells can occur across transient virological synapses . Finally, HIV-1 also appears able to hijack tunneling nanotubes for its own spreading .
Another important open question is why the viral assembly compartment occurs in an internal compartment in macrophages and not in T cells. Obviously something has to differ between the two cell types, leading to distinct trafficking events. Defining the molecular basis of these phenomena may provide valuable new therapeutic targets. Among many possible hypotheses to explain the specificity of the viral assembly in macrophages, a mechanism involving the miRNA pathway could be proposed. Indeed, miRNA expression patterns are modified by HIV-1 infection [93–96], and correlate with cell permissivity to HIV-1 in the monocyte/macrophage lineage .
In the future, new improvements of fluorescent microscopy allowing resolution close to tens of nanometers such as photoactivated localization microscopy  could be used for more precise localization of Gag and other viral components. Electron tomography as well as correlative light-electron microscopy could also be of interest, especially for the fine characterization of the relation between the viral assembly compartment and the plasma membrane. No doubt that the rapid development of imaging techniques, allowing the monitoring of dynamic and rapid events with high-resolution, will benefit the field of HIV assembly in primary cells and should yield very promising and exciting findings.
Endosomal Sorting Complex Required for Transport
Human Immunodeficiency Virus
- MHC II:
Major Histocompatibility Complex class II molecules
protein A coupled to gold particles
Gartner S, Markovits P, Markovitz DM, Kaplan MH, Gallo RC, Popovic M: The role of mononuclear phagocytes in HTLV-III/LAV infection. Science. 1986, 233: 215-219. 10.1126/science.3014648.
Orenstein JM, Meltzer MS, Phipps T, Gendelman HE: Cytoplasmic assembly and accumulation of human immunodeficiency virus types 1 and 2 in recombinant human colony-stimulating factor-1-treated human monocytes: an ultrastructural study. J Virol. 1988, 62: 2578-2586.
Sharova N, Swingler C, Sharkey M, Stevenson M: Macrophages archive HIV-1 virions for dissemination in trans. Embo J. 2005, 24: 2481-2489. 10.1038/sj.emboj.7600707.
Gonzalez-Scarano F, Martin-Garcia J: The neuropathogenesis of AIDS. Nat Rev Immunol. 2005, 5: 69-81. 10.1038/nri1527.
Ellery PJ, Tippett E, Chiu YL, Paukovics G, Cameron PU, Solomon A, Lewin SR, Gorry PR, Jaworowski A, Greene WC, Sonza S, Crowe SM: The CD16+ monocyte subset is more permissive to infection and preferentially harbors HIV-1 in vivo. J Immunol. 2007, 178: 6581-6589.
Gordon S, Taylor PR: Monocyte and macrophage heterogeneity. Nat Rev Immunol. 2005, 5: 953-964. 10.1038/nri1733.
Steinman RM, Mellman IS, Muller WA, Cohn ZA: Endocytosis and the recycling of plasma membrane. J Cell Biol. 1983, 96: 1-27. 10.1083/jcb.96.1.1.
Gendelman HE, Orenstein JM, Martin MA, Ferrua C, Mitra R, Phipps T, Wahl LA, Lane HC, Fauci AS, Burke DS: Efficient isolation and propagation of human immunodeficiency virus on recombinant colony-stimulating factor 1-treated monocytes. J Ex Med. 1988, 167: 1428-1441. 10.1084/jem.167.4.1428.
Raposo G, Moore M, Innes D, Leijendekker R, Leigh-Brown A, Benaroch P, Geuze H: Human Macrophages Accumulate HIV-1 Particles in MHC II Compartments. Traffic. 2002, 3: 718-729. 10.1034/j.1600-0854.2002.31004.x.
Pelchen-Matthews A, Kramer B, Marsh M: Infectious HIV-1 assembles in late endosomes in primary macrophages. J Cell Biol. 2003, 162: 443-455. 10.1083/jcb.200304008.
Gheysen D, Jacobs E, de Foresta F, Thiriart C, Francotte M, Thines D, De Wilde M: Assembly and release of HIV-1 precursor Pr55gag virus-like particles from recombinant baculovirus-infected insect cells. Cell. 1989, 59: 103-112. 10.1016/0092-8674(89)90873-8.
Freed EO: HIV-1 gag proteins: diverse functions in the virus life cycle. Virology. 1998, 251: 1-15. 10.1006/viro.1998.9398.
Bieniasz PD: The cell biology of HIV-1 virion genesis. Cell Host & Microbe. 2009, 5: 550-558. 10.1016/j.chom.2009.05.015.
Ono A: HIV-1 Assembly at the Plasma Membrane: Gag Trafficking and Localization. Future Virol. 2009, 4: 241-257. 10.2217/fvl.09.4.
Ganser-Pornillos BK, Yeager M, Sundquist WI: The structural biology of HIV assembly. Curr Opin Struct Biol. 2008, 18: 203-217. 10.1016/j.sbi.2008.02.001.
Klein KC, Reed JC, Lingappa JR: Intracellular destinies: degradation, targeting, assembly, and endocytosis of HIV Gag. AIDS reviews. 2007, 9: 150-161.
Lindwasser OW, Resh MD: Multimerization of human immunodeficiency virus type 1 Gag promotes its localization to barges, raft-like membrane microdomains. J Virol. 2001, 75: 7913-7924. 10.1128/JVI.75.17.7913-7924.2001.
Nermut MV, Zhang WH, Francis G, Ciampor F, Morikawa Y, Jones IM: Time Course of Gag Protein Assembly in HIV-1-Infected Cells: A Study by Immunoelectron Microscopy. Virology. 2003, 305: 219-227. 10.1006/viro.2002.1692.
Muriaux D, Mirro J, Harvin D, Rein A: RNA is a structural element in retrovirus particles. Proc Natl Acad Sci USA. 2001, 98: 5246-5251. 10.1073/pnas.091000398.
Hogue IB, Hoppe A, Ono A: Quantitative FRET Microscopy Analysis of HIV-1 Gag-Gag Interaction: The Relative Contributions of CA and NC Domains, and Membrane Binding. J Virol. 2009, 83: 7322-36. 10.1128/JVI.02545-08.
Poole E, Strappe P, Mok HP, Hicks R, Lever AM: HIV-1 Gag-RNA interaction occurs at a perinuclear/centrosomal site; analysis by confocal microscopy and FRET. Traffic. 2005, 6: 741-755. 10.1111/j.1600-0854.2005.00312.x.
Molle D, Segura-Morales C, Camus G, Berlioz-Torrent C, Kjems J, Basyuk E, Bertrand E: Endosomal trafficking of HIV-1 GAG and genomic RNAS regulates viral egress. J Biol Chem. 2009, 284: 19727-43. 10.1074/jbc.M109.019844.
Jin J, Sturgeon T, Weisz OA, Mothes W, Montelaro RC: HIV-1 matrix dependent membrane targeting is regulated by Gag mRNA trafficking. PLoS ONE. 2009, 4: e6551-10.1371/journal.pone.0006551.
Lehmann M, Milev MP, Abrahamyan L, Yao X-J, Pante N, Mouland AJ: Intracellular transport of human immunodeficiency virus type 1 genomic RNA and viral production are dependent on dynein motor function and late endosome positioning. J Biol Chem. 2009, 284: 14572-14585. 10.1074/jbc.M808531200.
McDonald B, Martin-Serrano J: No strings attached: the ESCRT machinery in viral budding and cytokinesis. J Cell Sci. 2009, 122: 2167-2177. 10.1242/jcs.028308.
Van Damme N, Goff D, Katsura C, Jorgenson RL, Mitchell R, Johnson MC, Stephens EB, Guatelli J: The interferon-induced protein BST-2 restricts HIV-1 release and is downregulated from the cell surface by the viral Vpu protein. Cell Host Microbe. 2008, 3: 245-252. 10.1016/j.chom.2008.03.001.
Van Damme N, Guatelli J: HIV-1 Vpu inhibits accumulation of the envelope glycoprotein within clathrin-coated, Gag-containing endosomes. Cell Microbiol. 2008, 10: 1040-1057. 10.1111/j.1462-5822.2007.01101.x.
Neil SJ, Eastman SW, Jouvenet N, Bieniasz PD: HIV-1 Vpu promotes release and prevents endocytosis of nascent retrovirus particles from the plasma membrane. PLoS Pathog. 2006, 2: e39-10.1371/journal.ppat.0020039.
Neil SJ, Zang T, Bieniasz PD: Tetherin inhibits retrovirus release and is antagonized by HIV-1 Vpu. Nature. 2008, 451: 425-430. 10.1038/nature06553.
Schindler M, Rajan D, Banning C, Wimmer P, Koppensteiner H, Iwanski A, Specht A, Sauter D, Dobner T, Kirchhoff F: Vpu serine 52 dependent counteraction of tetherin is required for HIV-1 replication in macrophages, but not in ex vivo human lymphoid tissue. Retrovirology. 2010, 7: 1-10.1186/1742-4690-7-1.
Mitchell RS, Katsura C, Skasko MA, Fitzpatrick K, Lau D, Ruiz A, Stephens EB, Margottin-Goguet F, Benarous R, Guatelli JC: Vpu antagonizes BST-2-mediated restriction of HIV-1 release via beta-TrCP and endo-lysosomal trafficking. PLoS Pathog. 2009, 5: e1000450-10.1371/journal.ppat.1000450.
Mangeat B, Gers-Huber G, Lehmann M, Zufferey M, Luban J, Piguet V: HIV-1 Vpu neutralizes the antiviral factor Tetherin/BST-2 by binding it and directing its beta-TrCP2-dependent degradation. PLoS Pathog. 2009, 5: e1000574-10.1371/journal.ppat.1000574.
Sato K, Yamamoto SP, Misawa N, Yoshida T, Miyazawa T, Koyanagi Y: Comparative study on the effect of human BST-2/Tetherin on HIV-1 release in cells of various species. Retrovirology. 2009, 6: 53-10.1186/1742-4690-6-53.
Adamson CS, Freed EO: Human immunodeficiency virus type 1 assembly, release, and maturation. Adv Pharmacol. 2007, 55: 347-387. full_text.
Mujawar Z, Rose H, Morrow MP, Pushkarsky T, Dubrovsky L, Mukhamedova N, Fu Y, Dart A, Orenstein JM, Bobryshev YV, Bukrinsky M, Sviridov D: Human immunodeficiency virus impairs reverse cholesterol transport from macrophages. PLoS Biol. 2006, 4: e365-10.1371/journal.pbio.0040365.
Ryzhova EV, Vos RM, Albright AV, Harrist AV, Harvey T, Gonzalez-Scarano F: Annexin 2: a novel human immunodeficiency virus type 1 Gag binding protein involved in replication in monocyte-derived macrophages. J Virol. 2006, 80: 2694-2704. 10.1128/JVI.80.6.2694-2704.2006.
Mayran N, Parton RG, Gruenberg J: Annexin II regulates multivesicular endosome biogenesis in the degradation pathway of animal cells. EMBO J. 2003, 22: 3242-3253. 10.1093/emboj/cdg321.
Batonick M, Favre M, Boge M, Spearman P, Honing S, Thali M: Interaction of HIV-1 Gag with the clathrin-associated adaptor AP-2. Virology. 2005, 342: 190-200. 10.1016/j.virol.2005.08.001.
Boge M, Wyss S, Bonifacino JS, Thali M: A membrane-proximal tyrosine-based signal mediates internalization of the HIV-1 envelope glycoprotein via interaction with the AP-2 clathrin adaptor. J Biol Chem. 1998, 273: 15773-15778. 10.1074/jbc.273.25.15773.
Byland R, Vance PJ, Hoxie JA, Marsh M: A conserved dileucine motif mediates clathrin and AP-2-dependent endocytosis of the HIV-1 envelope protein. Mol Biol Cell. 2007, 18: 414-425. 10.1091/mbc.E06-06-0535.
Camus G, Segura-Morales C, Molle D, Lopez-Verges S, Begon-Pescia C, Cazevieille C, Schu P, Bertrand E, Berlioz-Torrent C, Basyuk E: The clathrin adaptor complex AP-1 binds HIV-1 and MLV Gag and facilitates their budding. Mol Biol Cell. 2007, 18: 3193-3203. 10.1091/mbc.E06-12-1147.
Ohno H, Aguilar RC, Fournier MC, Hennecke S, Cosson P, Bonifacino JS: Interaction of endocytic signals from the HIV-1 envelope glycoprotein complex with members of the adaptor medium chain family. Virology. 1997, 238: 305-315. 10.1006/viro.1997.8839.
Wyss S, Berlioz-Torrent C, Boge M, Blot G, Honing S, Benarous R, Thali M: The highly conserved C-terminal dileucine motif in the cytosolic domain of the human immunodeficiency virus type 1 envelope glycoprotein is critical for its association with the AP-1 clathrin adapter. J Virol. 2001, 75: 2982-2992. 10.1128/JVI.75.6.2982-2992.2001.
Dong X, Li H, Derdowski A, Ding L, Burnett A, Chen X, Peters TR, Dermody TS, Woodruff E, Wang JJ, Spearman P: AP-3 directs the intracellular trafficking of HIV-1 Gag and plays a key role in particle assembly. Cell. 2005, 120: 663-674. 10.1016/j.cell.2004.12.023.
Joshi A, Garg H, Nagashima K, Bonifacino JS, Freed EO: GGA and Arf proteins modulate retrovirus assembly and release. Mol Cell. 2008, 30: 227-238. 10.1016/j.molcel.2008.03.015.
Lopez-Verges S, Camus G, Blot G, Beauvoir R, Benarous R, Berlioz-Torrent C: Tail-interacting protein TIP47 is a connector between Gag and Env and is required for Env incorporation into HIV-1 virions. Proc Natl Acad Sci USA. 2006, 103: 14947-14952. 10.1073/pnas.0602941103.
Nishi M, Ryo A, Tsurutani N, Ohba K, Sawasaki T, Morishita R, Perrem K, Aoki I, Morikawa Y, Yamamoto N: Requirement for microtubule integrity in the SOCS1-mediated intracellular dynamics of HIV-1 Gag. FEBS Lett. 2009, 583: 1243-1250. 10.1016/j.febslet.2009.03.041.
Leblanc JJ, Perez O, Hope T: Probing the structural states of human immunodeficiency virus type 1 pr55gag by using monoclonal antibodies. J Virol. 2008, 82: 2570-2574. 10.1128/JVI.01717-07.
Ryo A, Tsurutani N, Ohba K, Kimura R, Komano J, Nishi M, Soeda H, Hattori S, Perrem K, Yamamoto M, Chiba J, Mimaya J, Yoshimura K, Matsushita S, Honda M, Yoshimura A, Sawasaki T, Aoki I, Morikawa Y, Yamamoto N: SOCS1 is an inducible host factor during HIV-1 infection and regulates the intracellular trafficking and stability of HIV-1 Gag. Proc Natl Acad Sci USA. 2008, 105: 294-299. 10.1073/pnas.0704831105.
Tang Y, Winkler U, Freed EO, Torrey TA, Kim W, Li H, Goff SP, Morse HC: Cellular motor protein KIF-4 associates with retroviral Gag. J Virol. 1999, 73: 10508-10513.
Martinez NW, Xue X, Berro RG, Kreitzer G, Resh MD: Kinesin KIF4 regulates intracellular trafficking and stability of the human immunodeficiency virus type 1 Gag polyprotein. J Virol. 2008, 82: 9937-9950. 10.1128/JVI.00819-08.
Chertova E, Chertov O, Coren LV, Roser JD, Trubey CM, Bess JW, Sowder RC, Barsov E, Hood BL, Fisher RJ, Nagashima K, Conrads TP, Veenstra TD, Lifson JD, Ott DE: Proteomic and biochemical analysis of purified human immunodeficiency virus type 1 produced from infected monocyte-derived macrophages. J Virol. 2006, 80: 9039-9052. 10.1128/JVI.01013-06.
König R, Zhou Y, Elleder D, Diamond TL, Bonamy GMC, Irelan JT, Chiang C-Y, Tu BP, De Jesus PD, Lilley CE, Seidel S, Opaluch AM, Caldwell JS, Weitzman MD, Kuhen KL, Bandyopadhyay S, Ideker T, Orth AP, Miraglia LJ, Bushman FD, Young JA, Chanda SK: Global analysis of host-pathogen interactions that regulate early-stage HIV-1 replication. Cell. 2008, 135: 49-60. 10.1016/j.cell.2008.07.032.
Zhou H, Xu M, Huang Q, Gates A, Zhang X, Castle J, Stec E, Ferrer M, Strulovici B, Hazuda D, Espeseth A: Genome-Scale RNAi Screen for Host Factors Required for HIV Replication. Cell Host & Microbe. 2008, 4: 495-504. 10.1016/j.chom.2008.10.004.
Brass AL, Dykxhoorn DM, Benita Y, Yan N, Engelman A, Xavier RJ, Lieberman J, Elledge SJ: Identification of host proteins required for HIV infection through a functional genomic screen. Science. 2008, 319: 921-926. 10.1126/science.1152725.
Yeung ML, Houzet L, Yedavalli VSRK, Jeang K-T: A genome-wide short hairpin RNA screening of jurkat T-cells for human proteins contributing to productive HIV-1 replication. J Biol Chem. 2009, 284: 19463-19473. 10.1074/jbc.M109.010033.
Bushman FD, Malani N, Fernandes J, D'Orso I, Cagney G, Diamond TL, Zhou H, Hazuda DJ, Espeseth AS, König R, Bandyopadhyay S, Ideker T, Goff SP, Krogan NJ, Frankel AD, Young JA, Chanda SK: Host cell factors in HIV replication: meta-analysis of genome-wide studies. PLoS Pathog. 2009, 5: e1000437-10.1371/journal.ppat.1000437.
Kok K, Lei T, Jin D: siRNA and shRNA screens advance key understanding of host factors required for HIV-1 replication. Retrovirology. 2009, 6: 78-10.1186/1742-4690-6-78.
Briggs JA, Johnson MC, Simon MN, Fuller SD, Vogt VM: Cryo-electron microscopy reveals conserved and divergent features of gag packing in immature particles of Rous sarcoma virus and human immunodeficiency virus. Journal of Molecular Biology. 2006, 355: 157-168. 10.1016/j.jmb.2005.10.025.
Gousset K, Ablan SD, Coren LV, Ono A, Soheilian F, Nagashima K, Ott DE, Freed EO: Real-time visualization of HIV-1 GAG trafficking in infected macrophages. PLoS Pathog. 2008, 4: e1000015-10.1371/journal.ppat.1000015.
Kramer B, Pelchen-Matthews A, Deneka M, Garcia E, Piguet V, Marsh M: HIV interaction with endosomes in macrophages and dendritic cells. Blood Cells Mol Dis. 2005, 35: 136-142. 10.1016/j.bcmd.2005.06.006.
Welsch S, Habermann A, Jager S, Muller B, Krijnse-Locker J, Krausslich HG: Ultrastructural analysis of ESCRT proteins suggests a role for endosome-associated tubular-vesicular membranes in ESCRT function. Traffic. 2006, 7: 1551-1566. 10.1111/j.1600-0854.2006.00489.x.
Orenstein JM: Ultrastructure of HIV/AIDS. Ultrastruct Pathol. 2002, 26: 245-250. 10.1080/01913120290104502.
Welsch S, Keppler OT, Habermann A, Allespach I, Krijnse-Locker J, Krausslich HG: HIV-1 Buds Predominantly at the Plasma Membrane of Primary Human Macrophages. PLoS Pathog. 2007, 3: e36-10.1371/journal.ppat.0030036.
Jouve M, Sol-Foulon N, Watson S, Schwartz O, Benaroch P: HIV-1 Buds and Accumulates in "Nonacidic" Endosomes of Macrophages. Cell Host Microbe. 2007, 2: 85-95. 10.1016/j.chom.2007.06.011.
Nguyen DG, Booth A, Gould SJ, Hildreth JEK: Evidence that HIV budding in primary macrophages occurs through the exosome release pathway. J Biol Chem. 2003, 278: 52347-52354. 10.1074/jbc.M309009200.
Ono A, Freed EO: Cell-type-dependent targeting of human immunodeficiency virus type 1 assembly to the plasma membrane and the multivesicular body. J Virol. 2004, 78: 1552-1563. 10.1128/JVI.78.3.1552-1563.2004.
Nydegger S, Foti M, Derdowski A, Spearman P, Thali M: HIV-1 egress is gated through late endosomal membranes. Traffic. 2003, 4: 902-910. 10.1046/j.1600-0854.2003.00145.x.
Grigorov B, Arcanger F, Roingeard P, Darlix JL, Muriaux D: Assembly of infectious HIV-1 in human epithelial and T-lymphoblastic cell lines. J Mol Biol. 2006, 359: 848-862. 10.1016/j.jmb.2006.04.017.
Sherer NM, Lehmann MJ, Jimenez-Soto LF, Ingmundson A, Horner SM, Cicchetti G, Allen PG, Pypaert M, Cunningham JM, Mothes W: Visualization of retroviral replication in living cells reveals budding into multivesicular bodies. Traffic. 2003, 4: 785-801. 10.1034/j.1600-0854.2003.00135.x.
Perlman M, Resh MD: Identification of an intracellular trafficking and assembly pathway for HIV-1 gag. Traffic. 2006, 7: 731-745. 10.1111/j.1398-9219.2006.00428.x.
Joshi A, Ablan SD, Soheilian F, Nagashima K, Freed EO: Evidence that productive human immunodeficiency virus type 1 assembly can occur in an intracellular compartment. J Virol. 2009, 83: 5375-5387. 10.1128/JVI.00109-09.
Ongradi J, Ceccherini-Nelli L, Pistello M, Specter S, Bendinelli M: Acid sensitivity of cell-free and cell-associated HIV-1: clinical implications. AIDS Res Hum Retroviruses. 1990, 6: 1433-1436. 10.1089/aid.1990.6.1433.
Jouvenet N, Neil SJ, Bess C, Johnson MC, Virgen CA, Simon SM, Bieniasz PD: Plasma Membrane Is the Site of Productive HIV-1 Particle Assembly. PLoS Biol. 2006, 4: e435-10.1371/journal.pbio.0040435.
Marechal V, Prevost M-C, Petit C, Perret E, Heard J-M, Schwartz O: Human Immunodeficiency Virus Type 1 Entry into Macrophages Mediated by Macropinocytosis. J Virol. 2001, 75: 11166-11177. 10.1128/JVI.75.22.11166-11177.2001.
Harila K, Prior I, Sjoberg M, Salminen A, Hinkula J, Suomalainen M: Vpu and Tsg101 regulate intracellular targeting of the human immunodeficiency virus type 1 core protein precursor Pr55gag. J Virol. 2006, 80: 3765-3772. 10.1128/JVI.80.8.3765-3772.2006.
Deneka M, Pelchen-Matthews A, Byland R, Ruiz-Mateos E, Marsh M: In macrophages, HIV-1 assembles into an intracellular plasma membrane domain containing the tetraspanins CD81, CD9, and CD53. J Cell Biol. 2007, 177: 329-341. 10.1083/jcb.200609050.
Hayat MA: Principles and Techniques of Electron Microscopy: Biological Applications. 2000, Cambridge: Cambridge University Press, 4
Luft JH: Ruthenium red and violet. II. Fine structural localization in animal tissues. Anat Rec. 1971, 171: 369-415. 10.1002/ar.1091710303.
Bennett AE, Narayan K, Shi D, Hartnell LM, Gousset K, He H, Lowekamp BC, Yoo TS, Donald Bliss D, EO F, Subramaniam S: Ion-abrasion scanning electron microscopy reveals surface-connected tubular conduits in HIV-infected macrophages. PLOS Pathogens. 2009, 5 (9): e1000591-10.1371/journal.ppat.1000591.
Waheed AA, Freed EO: Lipids and membrane microdomains in HIV-1 replication. Virus Res. 2009, 143: 162-176. 10.1016/j.virusres.2009.04.007.
Ruiz-Mateos E, Pelchen-Matthews A, Deneka M, Marsh M: CD63 is not required for production of infectious human immunodeficiency virus type 1 in human macrophages. J Virol. 2008, 82: 4751-4761. 10.1128/JVI.02320-07.
Chen H, Dziuba N, Friedrich B, von Lindern J, Murray JL, Rojo DR, Hodge TW, O'Brien WA, Ferguson MR: A critical role for CD63 in HIV replication and infection of macrophages and cell lines. Virology. 2008, 379: 191-196. 10.1016/j.virol.2008.06.029.
Sachse M, Urbe S, Oorschot V, Strous GJ, Klumperman J: Bilayered Clathrin Coats on Endosomal Vacuoles Are Involved in Protein Sorting toward Lysosomes. Mol Biol Cell. 2002, 13: 1313-1328. 10.1091/mbc.01-10-0525.
Kruth HS: Sequestration of aggregated low-density lipoproteins by macrophages. Curr Opin Lipidol. 2002, 13: 483-488. 10.1097/00041433-200210000-00003.
Balliet JW, Kolson DL, Eiger G, Kim FM, McGann KA, Srinivasan A, Collman R: Distinct effects in primary macrophages and lymphocytes of the human immunodeficiency virus type 1 accessory genes vpr, vpu, and nef: mutational analysis of a primary HIV-1 isolate. Virology. 1994, 200: 623-631. 10.1006/viro.1994.1225.
Swingler S, Mann A, Jacque J, Brichacek B, Sasseville VG, Williams K, Lackner AA, Janoff EN, Wang R, Fisher D, Stevenson M: HIV-1 Nef mediates lymphocyte chemotaxis and activation by infected macrophages [see comments]. Nat Med. 1999, 5: 997-103. 10.1038/12433.
Jouvenet N, Bieniasz PD, Simon SM: Imaging the biogenesis of individual HIV-1 virions in live cells. Nature. 2008, 454: 236-240. 10.1038/nature06998.
Hubner W, McNerney GP, Chen P, Dale BM, Gordon RE, Chuang FY, Li XD, Asmuth DM, Huser T, Chen BK: Quantitative 3D video microscopy of HIV transfer across T cell virological synapses. Science. 2009, 323: 1743-1747. 10.1126/science.1167525.
Ostrowski M, Carmo NB, Krumeich S, Fanget I, Raposo G, Savina A, Moita CF, Schauer K, Hume AN, Freitas RP, Goud B, Benaroch P, Hacohen N, Fukuda M, Desnos C, Seabra MC, Darchen F, Amigorena S, Moita LF, Thery C: Rab27a and Rab27b control different steps of the exosome secretion pathway. Nature Cell Biology. 2009, 12 (1): 19-30. 10.1038/ncb2000. sup pp 1-13
Groot F, Welsch S, Sattentau QJ: Efficient HIV-1 transmission from macrophages to T cells across transient virological synapses. Blood. 2008, 111: 4660-4663. 10.1182/blood-2007-12-130070.
Sowinski S, Jolly C, Berninghausen O, Purbhoo MA, Chauveau A, Köhler K, Oddos S, Eissmann P, Brodsky FM, Hopkins C, Onfelt B, Sattentau Q, Davis DM: Membrane nanotubes physically connect T cells over long distances presenting a novel route for HIV-1 transmission. Nat Cell Biol. 2008, 10: 211-219. 10.1038/ncb1682.
Yeung ML, Bennasser Y, Myers TG, Jiang G, Benkirane M, Jeang KT: Changes in microRNA expression profiles in HIV-1-transfected human cells. Retrovirology. 2005, 2: 81-10.1186/1742-4690-2-81.
Yeung ML, Bennasser Y, Le SY, Jeang KT: RNA interference and HIV-1. Adv Pharmacol. 2007, 55: 427-438. full_text.
Triboulet R, Mari B, Lin YL, Chable-Bessia C, Bennasser Y, Lebrigand K, Cardinaud B, Maurin T, Barbry P, Baillat V, Reynes J, Corbeau P, Jeang KT, Benkirane M: Suppression of microRNA-silencing pathway by HIV-1 during virus replication. Science. 2007, 315: 1579-1582. 10.1126/science.1136319.
Houzet L, Yeung ML, de Lame V, Desai D, Smith SM, Jeang KT: MicroRNA profile changes in human immunodeficiency virus type 1 (HIV-1) seropositive individuals. Retrovirology. 2008, 5: 118-10.1186/1742-4690-5-118.
Wang X, Ye L, Hou W, Zhou Y, Wang YJ, Metzger DS, Ho WZ: Cellular microRNA expression correlates with susceptibility of monocytes/macrophages to HIV-1 infection. Blood. 2009, 113: 671-674. 10.1182/blood-2008-09-175000.
Betzig E, Patterson GH, Sougrat R, Lindwasser OW, Olenych S, Bonifacino JS, Davidson MW, Lippincott-Schwartz J, Hess HF: Imaging intracellular fluorescent proteins at nanometer resolution. Science. 2006, 313: 1642-1645. 10.1126/science.1127344.
The authors greatly acknowledge the Nikon Imaging Center @ Institut Curie-CNRS as well as the electron microscopy facility of the Curie. We thank Rhys Allan for correcting the English of the manuscript. EB and RG were supported by fellowships, and PB by grants from "Agence Nationale de Recherche contre le SIDA", and from "Ensemble Contre le SIDA". MS is supported by grants from the "Deutsche Forschungs Gemeinschaft" and the "Stiftung zur Bekämpfung neuroviraler Erkrankungen". We apologize to our colleagues whose work could not be cited owing to space constraints.
The authors declare that they have no competing interests.
PB wrote the manuscript and edited it, EB drew the figure 1 and helped to draft the manuscript, RG performed the figure 2, MS contributed to text edition, MJ performed all the EM techniques and produced the figures 3 and 4. All authors contributed to helpful discussions that enriched the review, and all authors approved the final manuscript.
About this article
Cite this article
Benaroch, P., Billard, E., Gaudin, R. et al. HIV-1 assembly in macrophages. Retrovirology 7, 29 (2010). https://doi.org/10.1186/1742-4690-7-29