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Volume 6 Supplement 3

AIDS Vaccine 2009

P04-46. Broadly cross-neutralizing activity in plasma of HIV-1 and HIV-2 infected individuals

Background

Inter-subtype neutralization of HIV-1 and cross-neutralization between HIV 1 and 2 was explored using plasma collected from 54 HIV-infected individuals within the framework of a case control study in Guinea-Bissau, West Africa. Twenty one were infected with HIV-1, 21 with HIV-2 and 12 were dually HIV-1/2 seropositive, of whom 5 were from a professional cohort in the same area. HTLV-I status was also known, five HIV-1-infected and 10 HIV-2-infected were HTLV-I seropositive.

Methods

The plasma were tested for neutralizing activity against five HIV-1 and one HIV-2 isolates. Two HIV-1 (subtype A/G) isolates and the HIV-2 isolate were from Guinea-Bissau, one HIV-1 subtype B isolate was from Sweden and two subtype C isolates were from Brazil and India, respectively. Plaque reduction assay on U87.CD4-CCR5 cells was used and neutralization was scored as negative (<30% neutralization), moderate (30–59% neutralization) and strong (≥60% neutralization).

Results

Sixty seven percent of HIV-1 plasma strongly neutralized at least one HIV-1 isolate, while 33% strongly neutralized at least two HIV-1 isolates. All HIV-2 plasma neutralized the HIV-2 isolate (70% strongly) and 29% strongly neutralized at least one HIV-1 isolate (5% neutralized at least two HIV-1 isolates). This is to be compared with 10% of HIV-1 plasma that neutralized the HIV-2 isolate.

Conclusion

HTLV-I co-infection appears to modulate neutralization in HIV-1 infection, whereas gender seems to influence neutralization by HIV-2 plasma.

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Correspondence to E Fenyö.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fenyö, E., Holmgren, B., da Silva, Z. et al. P04-46. Broadly cross-neutralizing activity in plasma of HIV-1 and HIV-2 infected individuals. Retrovirology 6, P74 (2009). https://doi.org/10.1186/1742-4690-6-S3-P74

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Keywords

  • Infectious Disease
  • Cancer Research
  • Case Control Study
  • Case Control
  • Infected Individual