Skip to main content

Volume 6 Supplement 3

AIDS Vaccine 2009

P04-21. Different human serum antibody profiles elicited by three candidate HIV vaccines using different immunization approaches


Study and direct comparison of HIV-1 Env specific antibody responses elicited by HIV vaccines can provide useful information to identify vaccine candidates in early stage trials with greater potential of providing a protective immune response. With the goal of describing qualitative differences in humoral responses elicited by disparate HIV vaccine delivery approaches, we evaluated serum samples from three independent trials: HVTN 041, a recombinant protein based vaccine; HVTN 203, a combination canarypox prime plus protein boost vaccine, and DP6-001, a DNA prime plus protein boost vaccine.


Humoral responses elicited by each vaccine trial were evaluated by ELISA for endpoint binding titer against a heterologous clade B antigen JR-FL and evaluated for antibody specificity using overlapping linear peptides and mAbs in a pseudoviral based competitive binding assay. Neutralizing specificity was evaluated against model clade B isolates in a pseudoviral system.


We determined that binding titers are not indicative of the domain specificities or neutralizing activities of the sera being generated through immunization. Analysis of antibody specificity indicated that all three trials elicited high titers of antibodies targeted to the V3 loop and co-receptor binding site of the HIV envelope. However, antibodies targeted to the CD4 binding domain were elicited significantly more frequently and in higher titer in individuals from the DP6-001 trial. Additionally, the presence of these CD4 binding site specific antibodies correlated with increased neutralization breadth against model clade B isolates.


In summary, this study confirmed in human volunteers that a DNA prime-protein boost regimen is highly capable of eliciting antibodies responses against key epitopes on HIV-1 Env. *HVTN041 and HVTN203 sera were kindly provided by HVTN Ancillary Study Group

Author information

Authors and Affiliations


Rights and permissions

Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution License ( ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Cite this article

Vaine, M., Wang, S. & Lu, S. P04-21. Different human serum antibody profiles elicited by three candidate HIV vaccines using different immunization approaches. Retrovirology 6 (Suppl 3), P49 (2009).

Download citation

  • Published:

  • DOI: