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Volume 6 Supplement 3

AIDS Vaccine 2009

P04-10. Neutralization of Tier 1 and Tier 2 pseudoviruses by human anti-V3 monoclonal antibodies

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Background

The structural conservation of the V3 region is related to its biological function, and explains the ability of many anti-V3 monoclonal Abs (mAbs) to cross-neutralize diverse HIV-1 strains. Experiments were undertaken to determine the proportion of Tier 1 and 2 viruses that are neutralized by anti-V3 mAbs

Methods

Eighteen anti-V3 mAbs were developed from the cells of individuals living in Cameroon, India and the New York City area, respectively. The mAb neutralizing activity was tested by titration in the TZM-bl cell assay against 34 Tier 1 and Tier 2 pseudoviruses (psVs) derived from the envelopes of viruses from clades A, AG, B and C. IC50 values of <50 μg mAb/ml were defined as positive for neutralization.

Results

All 10 Tier 1 psVs from clades A, AG, B and C were neutralized by one or more of the 18 mAbs. The median IC50 value for neutralization of Tier 1 psVs by anti-V3 mAbs was 0.8 ug/ml. Of the 24 Tier 2 viruses from clades B and C that constitute the current standard panels, 8 of 24 were neutralized by one or more mAbs. The median IC50 value for neutralizing anti-V3 mAbs against Tier 2 viruses was 34.5 μg/ml. The anti-V3 mAbs derived from Cameroonian subjects (mostly infected with AG viruses) showed the greatest breadth, neutralizing 3 clade C and 4 clade B Tier 2 viruses. Clade B-derived mAbs neutralized 1 clade C and 4 clade B psVs, and mAbs from Indian subjects neutralized just two clade C Tier 2 viruses.

Conclusion

Neutralization of 100% of Tier 1 and 33% of Tier 2 viruses in the standard panels by anti-V3 mAbs suggests that anti-V3 Abs will be a useful and important component of a protective vaccine-induced immune response.

Author information

Correspondence to MK Gorny.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Gorny, M., Williams, C., O'Neal, T. et al. P04-10. Neutralization of Tier 1 and Tier 2 pseudoviruses by human anti-V3 monoclonal antibodies. Retrovirology 6, P38 (2009). https://doi.org/10.1186/1742-4690-6-S3-P38

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Keywords

  • York City
  • Current Standard
  • City Area
  • Cell Assay
  • Neutralize Activity