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Volume 6 Supplement 3

AIDS Vaccine 2009

P20-06. Study of viral variability evolution in patients submitted to a therapeutic vaccine based on autologous dendritic cells pulsed with autologous HIV-1

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Background

A previous therapeutic vaccine pilot study based on autologous dendritic cells pulsed with autologous heat inactivated viruses HIV-1 obtained by plasmapheresis, showed a decrease in the plasma viral load in 4 of 12 chronically HIV-1-infected patients [García et al., J Infect Dis. 2005; 191: 1680–1685]. This vaccination strategy required to submit the infected patients to 2 structured treatment interruptions (STI): the first to recover enough viruses to be used as antigen, and the second to assess virological responses after the immunization. We aimed to study the viral variability evolution changes occurring during off therapy period, before and after vaccination.

Methods

12 patients on HAART underwent a HAART interruption (STOP1) to obtain the virus by plasmaphereses. Then, HAART was reinitiated and thereafter they received 4 immunizations. Six weeks after the last immunization, HAART was definitely interrupted (STOP2). 4 controls were submitted to both HAART interruptions but did not receive any vaccination.

Sequences corresponding to the envelope C2V5 region were analyzed from plasma samples obtained at baseline, STOP1 and STOP2. Frequency and complexity values were manually calculated. Genetic distances were conducted using MEGA software version 4 [Tamura et al., Mol Biol Evol., 2007; 24: 1596–1599].

Results

A high complexity was observed in all patients. The divergence rate between stops was higher than between baseline and STOP1, and divergence rate between baseline and STOP 2 was lower than between baseline and STOP1.

In the immunized patients, the viral complexity was lower in STOP2 than in STOP1, and there was a trend to decrease amino acid changes and diversity after this therapeutic vaccination.

Conclusion

All patients showed a great variability in all studied populations. We suggest that viruses observed in STOP2 are more related to the baseline than with the viruses found in STOP1 in all patients. Immunized patients showed lower viral complexity after the therapeutic vaccination.

Author information

Correspondence to M Guilà.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Guilà, M., Dalmau, J., Gil, C. et al. P20-06. Study of viral variability evolution in patients submitted to a therapeutic vaccine based on autologous dendritic cells pulsed with autologous HIV-1. Retrovirology 6, P376 (2009). https://doi.org/10.1186/1742-4690-6-S3-P376

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Keywords

  • Viral Load
  • Divergence Rate
  • Amino Acid Change
  • Virological Response
  • Vaccination Strategy