Skip to main content

Volume 6 Supplement 3

AIDS Vaccine 2009

P19-13. Recombinant proteins carrying peptide mimics of HIV-1


There are a number of monoclonal antibodies possessing neutralizing activity against the broad range of HIV-1 isolates. Some of them recognize conformational epitopes of HIV-1 antigens. Obviously, artificial anti-HIV polyepitope vaccines should induce a necessary spectrum of broadly neutralizing antibodies. Polyepitope TBI protein containing 4 T-cellular and 5 B-cellular linear epitopes from Env and Gag proteins of HIV-1 has been created earlier. Three linear peptides imitating natural HIV-1 conformational 2G12-determinant were selected using phage display and inserted in TBI.

The aim of our work was to study antigenic properties of these mimotopes in the context of newly created TBIs (TBI-1, TBI-2, and TBI-3).


Phage display was used to select peptides mimicking a HIV-1 gp120 conformational epitope recognized by neutralizing mAb 2G12.

Three linear antigenic determinants were found as a result of three rounds of biopanning. Oligonucleotides coding for these peptides-mimotopes were inserted into the TBI gene. Derivatives of TBI protein (TBI-1, TBI-2, TBI-3) containing mimotopes were expressed in E. coli and characterized by Western-blot and ELISA. Mice Balb/c were immunized by these recombinant proteins.


The newly created TBIs were shown to react in ELISA and Western-blot with 2G12 mAb, which means that they maintain original antigenic features of selected mimotopes. The sera of immunized animals recognise in ELISA both peptides-mimotopes for 2G12 as well as native HIV-1 antigen. The study of virus-neutralising activity of sera obtained is planning in the nearest future.


The selected peptides imitating natural epitopes for 2G12 mAb may be proposed as components of polyepitope anti-HIV vaccine.

Author information

Authors and Affiliations


Rights and permissions

Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Cite this article

Chikaev, A., Scherbakova, N., Tumanova, O. et al. P19-13. Recombinant proteins carrying peptide mimics of HIV-1. Retrovirology 6 (Suppl 3), P333 (2009).

Download citation

  • Published:

  • DOI: