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Volume 6 Supplement 3

AIDS Vaccine 2009

P17-10. A new AuxoGTU-HIV B DNA vaccine induce very long lasting HIV specific T cells response which is efficiently boosted with HIV LAI lipopeptides

Background

Prime-boost strategies are thought to increase the durability and the breadth of vaccine induced responses and we have recently shown that the use of a novel DNA vector, auxo-GTU®-MultiHIV-B is able to induce high level and long-lasting HIV specific T-cell responses in macaques. In addition, lipopeptides based on a palmitoyl group can efficiently process and present peptides by dendritic cells to CD8+T cells and have been shown immunogenic in animal models and in human.

The objective of this study was to boost auxo-GTU®-MultiHIV-B induced T-cell responses with lipopeptides based HIV-1 vaccine after 2 years of last DNA vaccine injection.

Methods

Cynomolgus macaques were primed with three (weeks 0, 4 and 12) intradermal injections (ID) with or without electroporation of auxo-GTU®-MultiHIV-B DNA vector. Animals received two distant booster injections of a multi-component vaccine composed of 5 HIV LAI lipopeptides containing peptides derived from HIV-1 Gag, Nef and Pol proteins.

Results

ID with auxo-GTU-MultiHIV-B DNA followed by EP induced IFNγ producing cells lasting for 3 years after the last plasmid injection. Production of IFNγ by PBMC was observed in response to all injected lipopeptides. After a single injection of Lipo5, we observed an increase in IFNγ production of PBMC against Gag17-35 and Gag253-284 lipopeptides in auxo-GTU-MultiHIV-B primed animals. A second Lipo5 injection increased these responses and induced IFNγ responses against Nef66-97 and Nef116-145 lipopeptides. Furthermore, we observed that two injections of Lipo5 were sufficient to induce IFNγ response against the 5 studied lipopeptides in one naïve macaque indicating that Lipo5 can prime an HIV specific response in macaques.

Conclusion

HIV-1 based lipopeptides are able to induce poly-epitopic T-cell responses in macaques and efficiently boost responses primed two years ago by DNA vaccination.

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Correspondence to I Mangeot-Méderlé.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Mangeot-Méderlé, I., Sylla, N., Corneau, A. et al. P17-10. A new AuxoGTU-HIV B DNA vaccine induce very long lasting HIV specific T cells response which is efficiently boosted with HIV LAI lipopeptides. Retrovirology 6, P292 (2009). https://doi.org/10.1186/1742-4690-6-S3-P292

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Keywords

  • Lipopeptides
  • Cynomolgus Macaque
  • Vaccine Injection
  • Plasmid Injection
  • Palmitoyl Group