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Volume 6 Supplement 3

AIDS Vaccine 2009

P16-53. Distinct subsets of memory T lymphocytes from HIV-1-infected subjects secrete IFN-γ and IL-2 in response to novel CD4+ T-cell HIV-1 epitopes

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Background

Functional memory T lymphocytes are involved in the control of HIV replication. Recently, we have described 18 HLA-DR promiscuous epitopes from HIV-1 consensus B sequences. Some of them contain CD8 epitopes inserted.

Objective

To investigate the functionality of the subsets of memory T cells that respond to these peptides among PBMC from HIV+ patients.

Methods

PBMC from 35 HIV+ patients with CD4 count>250 cells/μL: 8 aviremics in ART (AV-ART), 8 viremics in ART (VI-ART) (VL = 2260–31000 copies/mL) and 9 viremics naïve of therapy (VI-NO ART) (VL = 17100–698000 copies/mL) and 7 healthy donors were stimulated with pooled HIV- or CMV-peptides. The cellular phenotype and cytokine production were evaluated by multiparameter flow cytometry.

Results

The phenotypic analysis showed a significant reduction of CD8+ T naive cells in VI-ART (P = 0.014) and VI-NO ART (P = 0.003) compared to healthy group. The functional analysis revealed that all HIV-infected patients of our study had CD4+ and CD8+ secreting IFN-γ and/or IL-2 in response to our pool of HIV-1 peptides. This recognition involved central memory (TCM, CCR7+CD45RA-), effector memory (TEM, CCR7-CD45RA-), and CD45RA+ effector memory (TEMRA, CCR7-CD45RA+). In the CD4+ compartment the VI-ART group showed the smallest frequency of functional memory in response to our HIV pooled peptides. This observation was more evident in respect to IL-2 producing cells.

Conclusion

Our results suggest a functional heterogeneity of T memory subsets in response to set HIV-1 peptides, which may constitute a new candidate to an anti-HIV vaccine.

Author information

Correspondence to S Fonseca.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fonseca, S., Coutinho-Silva, A., Rodrigues, D. et al. P16-53. Distinct subsets of memory T lymphocytes from HIV-1-infected subjects secrete IFN-γ and IL-2 in response to novel CD4+ T-cell HIV-1 epitopes. Retrovirology 6, P282 (2009). https://doi.org/10.1186/1742-4690-6-S3-P282

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Keywords

  • Effector Memory
  • Cellular Phenotype
  • Central Memory
  • Functional Heterogeneity
  • Pool Peptide