P16-51. Functional characterization of novel SIV epitope specific T cells
Retrovirology volume 6, Article number: P280 (2009)
SIV infection of the rhesus macaque is currently the best animal model available for HIV infection in humans. However, the regions of SIV that are targeted by T cells, and the subsequent impact of specific targeting on disease progression, are insufficiently documented.
Serially truncated SIVmac239 peptides were used to identify novel epitopes to the 8 mer level, in an IFN-γ ELISPOT assay. MHC restriction analysis was performed using autologous and mismatched B cell lines. Epitope specific T cell lines were analysed for inhibition of virus replication in vitro in a virus suppression assay. Polychromatic flow cytometry was used to identify functional characteristics of epitope specific cells.
Novel epitope specific CD8 T cell responses were identified to the 8 mer level in Indian rhesus macaques vaccinated with SIVmac239 genes as part of a pre-clinical vaccine study, using IFN-γ ELISPOT. The functional properties and differential impact of epitope specific cells on virus replication will be discussed.
The SIVmac239 proteome remains largely unmapped, despite this virus being used as a model in the rhesus macaque for HIV infection. This study provides an insight into novel SIV epitopes and the impact that targeting may have on disease progression.
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Winstone, N., Mullen, K., Wilson, A. et al. P16-51. Functional characterization of novel SIV epitope specific T cells. Retrovirology 6 (Suppl 3), P280 (2009). https://doi.org/10.1186/1742-4690-6-S3-P280