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Volume 6 Supplement 3

AIDS Vaccine 2009

P11-17. Intermittent rectal shedding of multiple human adenovirus serotypes among HIV-positive MSM

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Background

The association between prior seropositivity to human adenovirus (HAd) type 5 and increased HIV acquisition in the Step Vaccine Study has raised questions concerning the frequency of endemic HAd infection in adults. Few studies have documented the frequency and types of HAd infections in HIV-infected populations using sensitive molecular methods. In this study we retrospectively tested rectal swabs collected from a prospective cohort of Peruvian HIV-positive MSM for the presence of HAds.

Methods

Swabs were collected from 20 individuals (median CD4+ T cells 406/μl, median plasma HIV viral load 4.3 log10 cop/ml) three times/week for 18 weeks. Viral DNA was eluted from the swabs in 1 ml proteinase K, and amplified using a sensitive multiplex PCR assay that detects all currently recognized HAd serotypes. Molecular typing of identified viruses was performed on a subset of samples by partial-length hexon gene sequencing.

Results

Fifteen of the twenty individuals (75%) shed HAd intermittently. CD4 count and plasma HIV-1 viral load did not differ between participants with and without HAd shedding (Mann-Whitney, p = 0.349 and p = 0.497, resp.). Among shedders, HAd was detected in 30% of samples (range 2.0% to 64.7%) with a mean of 6.1 × 103 adenovirus copies/ml of eluate (range 1.51 × 102 – 2.2 × 108 copies/ml). HAd shedding typically occurred on consecutive days in distinctly clustered episodes lasting a median of 4 days (range 1 to 9 days) separated by periods without shedding. At least 21 HAd types from species B, C, and D were identified, including Ad5, Ad26 and Ad48. Eight of fifteen shedders (53%) had more than one serotype present.

Conclusion

In conclusion, we found a high prevalence of rectal shedding of diverse human adenoviruses among HIV-positive MSM, including serotypes presently under consideration as vaccine vectors.

Author information

Correspondence to M Curlin.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Curlin, M., Celum, C., Sanchez, J. et al. P11-17. Intermittent rectal shedding of multiple human adenovirus serotypes among HIV-positive MSM. Retrovirology 6, P162 (2009). https://doi.org/10.1186/1742-4690-6-S3-P162

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Keywords

  • Viral Load
  • Molecular Typing
  • Rectal Swab
  • Human Adenovirus
  • Vaccine Vector