Volume 6 Supplement 2

Frontiers of Retrovirology: Complex retroviruses, retroelements and their hosts

Open Access

Inactivation of cell associated-HIV-1 in breast milk by treatment with the alkyl sulfate microbicide sodium dodecyl sulfate (SDS)

  • Edouard Tuaillon1, 2,
  • Kuda Mutasa3,
  • Pierre-Alain Rubbo1,
  • Laura Choteau2,
  • Florence Naudan2,
  • Karine Bollore1,
  • Jean-Pierre Vendrell1, 2 and
  • Philippe Van de Perre1, 2
Retrovirology20096(Suppl 2):P85

https://doi.org/10.1186/1742-4690-6-S2-P85

Published: 24 September 2009

Background

Breast milk is recognized as a predominant mode of HIV-1 infection in infants. Cell-associated HIV-1 may be the main source of virus transmission during early phases of breast-feeding. We have previously observed that HIV-1-infected cells spontaneously producing virus persist in breast-milk from women under antiretroviral therapy. Treatment of expressed milk with a microbicide such as Sodium dodecyl sulfate (SDS) is proposed as a simple and safe option to inactivate both cell free and cell associated HIV-1 when formula feeding is not practicable. However, the effect of SDS on spontaneously HIV-1-producing CD4+T cells in breast milk has not been fully explored.

Materials and methods

In this report human milk was spiked by HIV-1-infected cells and treated with increasing exposure time and SDS concentration. CD4+T cell apoptosis and death, cell-associated HIV-1 RNA production, and spontaneous HIV-1-Ag cell secretion were quantified after SDS treatment.

Results

Cell death increases in presence of SDS in a concentration- and time-dependent manner, 50% of T lymphocytes death after 2 minutes with 0.14% SDS and 90% after 10 minutes with 0.1% SDS (Fig. 1A). Undetectable HIV-1 RNA cell production was achieved following exposure with a minimum concentration of 0.1% SDS during 2 minutes, IC50 = 0.03% (Fig. 1B). The inhibition of HIV-1 Ag secretion was explored at a single cell level by ELISpot assay. Using this method inactivation was 100% for SDS concentrations ≥0.25% within 2 min (Fig. 1C).
Figure 1

Effect of SDS exposure on T cell death (A), cell-associated HIV-1 RNA production (B), and sponataeous HIV-1-Ag cell secretion (C).

Conclusion

By comparison with results previously reported using an infectivity model based on β-galactosidase MAGI cells1, we observed that a two fold higher SDS concentration was required to complete inactivation of HIV-1-Ag-secreting cells. This concentration remains in the reported safe limits for ingestion of SDS by children (1 g/kg/day). Regarding the possible occurrence of transmission to the infant after controlling cell-free virus in breast milk from women on antiretroviral therapy, SDS treatment of expressed breast milk may be an interesting strategy to optimized the prevention of HIV-1 pediatric transmission.

Authors’ Affiliations

(1)
Université Montpellier 1, EA 4205 Transmission, Pathogenèse et Prévention de l'Infection par le VIH
(2)
Laboratoire de Bactériologie-Virologie
(3)
ZVITAMBO project

References

  1. Urdaneta S, Wigdahl B, Neely EB, Berlin CM, Schengrund CL, Lin HM, Howett MK: Inactivation of HIV-1 in breast milk by treatment with the alkyl sulfate microbicide sodium dodecyl sulfate (SDS). Retrovirology. 2005, 2: 28-10.1186/1742-4690-2-28.PubMed CentralView ArticlePubMedGoogle Scholar

Copyright

© Tuaillon et al; licensee BioMed Central Ltd. 2009

This article is published under license to BioMed Central Ltd.

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