- Poster presentation
- Open Access
Inactivation of cell associated-HIV-1 in breast milk by treatment with the alkyl sulfate microbicide sodium dodecyl sulfate (SDS)
Retrovirology volume 6, Article number: P85 (2009)
Breast milk is recognized as a predominant mode of HIV-1 infection in infants. Cell-associated HIV-1 may be the main source of virus transmission during early phases of breast-feeding. We have previously observed that HIV-1-infected cells spontaneously producing virus persist in breast-milk from women under antiretroviral therapy. Treatment of expressed milk with a microbicide such as Sodium dodecyl sulfate (SDS) is proposed as a simple and safe option to inactivate both cell free and cell associated HIV-1 when formula feeding is not practicable. However, the effect of SDS on spontaneously HIV-1-producing CD4+T cells in breast milk has not been fully explored.
Materials and methods
In this report human milk was spiked by HIV-1-infected cells and treated with increasing exposure time and SDS concentration. CD4+T cell apoptosis and death, cell-associated HIV-1 RNA production, and spontaneous HIV-1-Ag cell secretion were quantified after SDS treatment.
Cell death increases in presence of SDS in a concentration- and time-dependent manner, 50% of T lymphocytes death after 2 minutes with 0.14% SDS and 90% after 10 minutes with 0.1% SDS (Fig. 1A). Undetectable HIV-1 RNA cell production was achieved following exposure with a minimum concentration of 0.1% SDS during 2 minutes, IC50 = 0.03% (Fig. 1B). The inhibition of HIV-1 Ag secretion was explored at a single cell level by ELISpot assay. Using this method inactivation was 100% for SDS concentrations ≥0.25% within 2 min (Fig. 1C).
By comparison with results previously reported using an infectivity model based on β-galactosidase MAGI cells1, we observed that a two fold higher SDS concentration was required to complete inactivation of HIV-1-Ag-secreting cells. This concentration remains in the reported safe limits for ingestion of SDS by children (1 g/kg/day). Regarding the possible occurrence of transmission to the infant after controlling cell-free virus in breast milk from women on antiretroviral therapy, SDS treatment of expressed breast milk may be an interesting strategy to optimized the prevention of HIV-1 pediatric transmission.
Urdaneta S, Wigdahl B, Neely EB, Berlin CM, Schengrund CL, Lin HM, Howett MK: Inactivation of HIV-1 in breast milk by treatment with the alkyl sulfate microbicide sodium dodecyl sulfate (SDS). Retrovirology. 2005, 2: 28-10.1186/1742-4690-2-28.