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MMTV-related sequences in feline breast carcinomas
Retrovirology volume 6, Article number: P72 (2009)
Mouse mammary tumor virus (MMTV) has a proven role in breast cancer (BC) in susceptible murine lines. MMTV-related sequences were also found in significant proportion of human sporadic and familial BC patients (17-52%). MMTV-homologous sequences were found in liver, timus and spleen of wild commensal mice; in 63% animals analyzed the sequences were expressed [1–3]. However, the role and origin of MMTV-related infection in humans is unclear.
Materials and methods
Earlier gag MMTV gene-related fragments were revealed in cats by other investigators . We studied 9 female cats (7-15 years old) with BC treated at the Laboratory Animals Brunch to search MMTV-homologous sequences in tumor cells of BC animals. Specific PCRs with primers for gp52-coding area of the env MMTV and gag MMTV genes were used. PCR-products were sequenced and partially cloned in pGEM-T vector followed by sequencing. All experiments were isolated from ones with mice or infectious MMTV to avoid DNA contamination.
MMTV-related sequences were revealed in DNA samples from tumor and adjacent breast tissues in 3/9 (33.3%) of BC animals. The sequences analysis by BLAST and DNA Star was shown 92-97% homology of the gp52 env MMTV-related area with exogenous MMTV (C3H and HeJ strains), 93-97% - with Mus musculus cDNA and 93-98% homology with human MMTV-related betaretrovirus. Homology of the gag MMTV-related area with exogenous MMTV was 92-95%, with Mus musculus cDNA - 96-98% and with human MMTV-like betaretrovirus - 97-99%. Homology of the env and gag MMTV genes - homologous sequences between different animals was 92-93% and 97%, correspondingly; ORFs were found in sequenced PCR products.
Preliminary data show high similarity between feline and human MMTV-related sequences revealed in BC tissues. Feline might to be intermediate host for viral infection and transmission from mice to human.
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Lushnikova ÀA, Kryukova IN, Peredereeva EV, et al: MMTV-related sequences in Mus musculus genome and human breast cancer tissue. Breast Cancer and Treatment, SABCS Abstracts, # 3087. 2008
Imai Sh, Okumoto M, Iwai M, et al: Distribution of mouse mammary tumor virus in Asian wild mice. J Virol. 1994, 68 (5): 3437-42.
Szabo S, Haislip AM, Traina-Dorge V, et al: Human, rhesus macaque, and feline sequences highly similar to mouse mammary tumor virus sequences. Microsc Res Tech. 2005, 68 (3-4): 209-221. 10.1002/jemt.20233.
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Cite this article
Lushnikova, À.A., Preobrazenskaya, A.B., Pavlov, V.A. et al. MMTV-related sequences in feline breast carcinomas. Retrovirology 6, P72 (2009). https://doi.org/10.1186/1742-4690-6-S2-P72
- Breast Cancer
- Breast Cancer
- Breast Carcinoma
- Intermediate Host
- Breast Cancer Tissue