Human herpesvirus 8 (HHV-8) “in vitro” infection of human placental histocultures
© Di Stefano et al.; licensee BioMed Central Ltd. 2008
Published: 9 April 2008
Most human Herpesvirus infect placental cells and may be harmful in pregnancy, leading to obstetrical and/or neonatal complications. Although a correlation between human herpesvirus 8 (HHV-8) infection and abortion or low birth weight in children has been reported [1, 2] presently no information has been published regarding HHV-8 tropism for placenta.
Materials and methods
In this study, a placenta histoculture system was used to evaluate the susceptibility of placental cells to “in vitro” HHV-8 infection. Quantitative detection of HHV-8 was performed by real-time PCR, and virus expression was evaluated by immunohistochemistry for latent and lytic HHV-8 antigens.
We here demonstrated for the first time that HHV-8, like other human herpesviruses, may productively infect placental cells in vitro, thus providing evidence that this phenomenon might influence vertical transmission and pregancy outcome in HHV-8-infected women.
This work was supported by grants from Istituto Superiore di Sanità (grant n. 50G.3, MLC; grant n. 50G.29, JRF) and from Associazione Italiana per la Ricerca sul Cancro (MLC). MB was the recipient of a fellowship from Associazione Italiana per la Lotta contro le Leucemie, Linfomi e Mieloma (AIL).
- Gaye-Diallo A, Toure AT, Gessain A, Gueye-Ndiaye A, Ndour AN, et al: Preliminary study of human Herpesvirus type 8 infection in pregnant women in Dakar (Senegal). Bull Soc Pathol Exot. 2001, 94: 231-234.PubMedGoogle Scholar
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