Virus phenotype variability during disease progression of HIV-1 infected children
© Cavarelli et al.; licensee BioMed Central Ltd. 2008
Published: 9 April 2008
HIV-1 infected children display different clinical evolution, i.e., “fast progression” (FP), “slow progression” (SP) and “long term non progression” (LTNP). One important phenotypic trait linked to disease progression is the evolution of the viral co-receptor use , involving a change from CCR5 to CXCR4 use . However, AIDS symptoms can appear in absence of X4 viruses. Recently chimeric receptors between CCR5 and CXCR4 were developed, in which subsequent parts of CCR5 were replaced with corresponding parts of CXCR4 . Their use allowed to document the biological variability of R5 isolates during the pathogenic process in adults .
To examine the HIV biological variability in children with different modes of disease progression.
Materials and methods
isolates from19 HIV-1 positive children were tested for their ability to infect U87.CD4+ cells expressing the wild type receptor CCR5, CXCR4, or one of the 6 chimeric CCR5/CXCR4 receptors.
Early during infection, all the viruses isolated from 8 SP children used only wild type CCR5 (called R5narrow). In one case, this phenotype persisted during disease progression, whereas in 2 children the virus evolved and was able to use multiple chimeric receptors (called R5broad), and in additional 5 children the virus evolved to CXCR4 usage. Interestengly the FP children, carried close to birth in 2 cases R5narrow virus, in 2 cases R5broad and in one case a dualtropic R5X4 virus. Virus with R5narrow evolved to R5broad in one of the 2 children carrying such phenotype. Both children with R5broad phenotype developed CXCR4 variants during the follow-up.
Evolution was observed also in the LTNP, although followed from later on in life (>8 years of age): all tested isolates from 2/6 LTNP remained R5narrow during disease progression; in one child an evolution from R5narrow to R5broad was observed whereas in another child the virus evolved from R5narrow to R5X4. The remaining two children showed R5broad phenotype during the whole follow-up.
Our results show that HIV-1 with broad chimeric receptor use is not hampered in transmission, and is more frequent close to birth in FP than in SP children. Viruses from LTNP show a similar phenotypic evolution though at later age.
- Bjorndal A, Deng H, Jansson M, Fiore JR, Colognesi C, Karlsson A, Albert J, Scarlatti G, Littman DR, Fenyo EM: Coreceptor usage of primary human immunodeficiency virus type 1 isolates varies according to biological phenotype. J Virol. 1997, 71: 7478-7487.PubMed CentralPubMedGoogle Scholar
- Karlsson I, Antonsson L, Shi Y, Karlsson A, Albert J, Leitner T, Olde B, Owman C, Fenyo EM: HIV biological variability unveiled: frequent isolations and chimeric receptors reveal unprecedented variation of coreceptor use. AIDS. 2003, 17: 2561-2569. 10.1097/00002030-200312050-00003.View ArticlePubMedGoogle Scholar
- Antonsson L, Boketoft A, Garzino-Demo A, Olde B, Owman C: Molecular mapping of epitopes for interaction of HIV-1 as well as natural ligands with the chemokine receptors, CCR5 and CXCR4. Aids. 2003, 17: 2571-2579. 10.1097/00002030-200312050-00004.View ArticlePubMedGoogle Scholar
- Karlsson I, Antonsson L, Shi Y, Oberg M, Karlsson A, Albert J, Olde B, Owman C, Jansson M, Fenyö EM: Coevolution of RANTES sensitivity and mode of CCR5 receptor use by human immunodeficiency virus type 1 of the R5 phenotype. J Virol. 2004, 78: 11807-11815. 10.1128/JVI.78.21.11807-11815.2004.PubMed CentralView ArticlePubMedGoogle Scholar
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