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Co-infection with Trypanosoma cruzi (Chagas' disease agent) decreases HIV-1 transcription in human placenta

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Background

Several factors determine the risk of HIV mother-to-child transmission (MTCT), such as co-infections in placentae from HIV-1 positive mothers with other pathogens [1, 2]. One of the most important endemic zoonosis in Latin America is Chagas' disease, caused by the protozoa Trypanosoma cruzi. MTCT of T. cruzi is today one of the main transmission routes in big cities [3]. The aim of the study was to determine whether T. cruzi modifies HIV infection at tissue or cellular level of the placenta.

Material and methods

Simple and double infections were carried out on a placental histoculture system (chorionic villi isolated from term placentae from HIV and Chagas negative mothers) and on choriocarcinoma BeWo cell line. We used trypomastigotes of T. cruzi (VD lethal strain, isolated from a child with MTCT), either purified from mice blood or from Vero cell cultures, 24h-supernatants of blood and cellular trypomastigotes, and the viral HIV-1DenvLuc+/VSV-G pseudotype. Viral replication was evaluated by luciferase activity quantification. Tissue viability was evaluated by hCG hormone secretion in histoculture supernatant. Quantification of soluble factors protein secretion and mRNA expression in histocultures were carried out by ELISAs and real time PCR respectively.

Results

Whole trypomastigotes, either from mice blood (mean ± SD; −92.13% ± 4.85) or from cell cultures (−97.33% ± 0.58), in co-infection with viral pseudotype decreased luciferase activity in placental histocultures. Similar results were obtained on BeWo cells. When supernatants from blood trypomastigotes were used on placental histocultures, luciferase activity presented a decrease of −85.50% ± 2.12, while supernatants from culture trypomastigotes presented lower effects (−65.50% ± 2.12). Tissue viability was not modified by viral and/or parasite infection. In co-infected histocultures, both protein secretion and mRNA expression of IL-10 were down regulated. Surprisingly, RANTES was increased.

Conclusions

Acute infection with T. cruzi and HIV-1 in this placentae in vitro system as well as in the trophoblast cell line decreased HIV-1 transcription. T. cruzi activity on HIV-1 replication seems to be caused not only by active infection but also by soluble factors shed by the parasite. A balance between proinflammatory and inhibitory cytokines/chimiokines might have a role in this phenomenon. Ongoing experiments are being conducted in order to elucidate the mechanisms involved in the impairment of HIV replication by T. cruzi and their role on MTCT of both pathogens.

References

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    Gallagher M, Malhotra I, Mungai PL, Wamachi AN, Kioko JM, Ouma JH, Muchiri E, King CL: The effects of maternal helminth and malaria infections on mother-to-child HIV transmission. AIDS. 2005, 19: 1849-1855. 10.1097/01.aids.0000189846.90946.5d.

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    Ayisi JG, van Eijk AM, Newman RD, ter Kuile FO, Shi YP, Yang C, Kolczak MS, Otieno JA, Misore AO, Kager PA, Lal RB, Steketee RW, Nahlen BL: Maternal malaria and perinatal HIV transmission, western Kenya. Emerg Infect Dis. 2004, 10: 643-652.

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    Carlier Y, Pinto Dias JC, Ostermayer Luquetti A, Hontebeyrie M, Torrico F, Truyens C: Trypanosomiase américaine ou maladie de Chagas. Maladies infectieuses, Paris. 2002, 8-505-A-20: 1-21. Editions Scientifiques et Médicales Elsevier SAS

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Author information

Correspondence to Guillermina Dolcini.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Dolcini, G., Solana, M.E., Andreani, G. et al. Co-infection with Trypanosoma cruzi (Chagas' disease agent) decreases HIV-1 transcription in human placenta. Retrovirology 5, O13 (2008) doi:10.1186/1742-4690-5-S1-O13

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Keywords

  • Luciferase Activity
  • Choriocarcinoma
  • Trypanosoma Cruzi
  • BeWo Cell
  • Viral Pseudotype