- Open Access
Lysine methylation of HIV-1 Tat regulates transcriptional activity of the viral LTR
© Van Duyne et al; licensee BioMed Central Ltd. 2008
- Received: 03 January 2008
- Accepted: 22 May 2008
- Published: 22 May 2008
The rate of transcription of the HIV-1 viral genome is mediated by the interaction of the viral protein Tat with the LTR and other transcriptional machinery. These specific interactions can be affected by the state of post-translational modifications on Tat. Previously, we have shown that Tat can be phosphorylated and acetylated in vivo resulting in an increase in the rate of transcription. In the present study, we investigated whether Tat could be methylated on lysine residues, specifically on lysine 50 and 51, and whether this modification resulted in a decrease of viral transcription from the LTR.
We analyzed the association of Tat with histone methyltransferases of the SUV39-family of SET domain containing proteins in vitro. Tat was found to associate with both SETDB1 and SETDB2, two enzymes which exhibit methyltransferase activity. siRNA against SETDB1 transfected into cell systems with both transient and integrated LTR reporter genes resulted in an increase in transcription of the HIV-LTR in the presence of suboptimal levels of Tat. In vitro methylation assays with Tat peptides containing point mutations at lysines 50 and 51 showed an increased incorporation of methyl groups on lysine 51, however, both residues indicated susceptibility for methylation.
The association of Tat with histone methyltransferases and the ability for Tat to be methylated suggests an interesting mechanism of transcriptional regulation through the recruitment of chromatin remodeling proteins to the HIV-1 promoter.
- Long Terminal Repeat
- Mutant Peptide
- TNE50 Buffer
- siRNA Pool
- Long Terminal Repeat Activity
The HIV-1 genome incorporates nine viral genes, all of which are expressed from a single promoter located within the viral long terminal repeat (LTR) [1, 2]. The activity of the HIV-1 promoter is strongly dependant on the viral transactivator, Tat, the protein responsible for transcriptional activation and elongation [3–8]. The main function of Tat is to activate the HIV-1 LTR by binding to an RNA stem-loop structure, TAR [3, 4, 6, 9–11]. This interaction initiates a binding cascade where cellular transcription factors such as Cdk9 and cyclin T1 are recruited to the HIV-1 promoter to facilitate viral transcription [12–15]. Tat mediates the functional modifications associated with viral transcription primarily by interacting with host cellular kinases, specifically to phosphorylate the large subunit of RNA Pol II CTD resulting in the activation of elongation [12, 16, 17]. In addition to the recruitment of host cellular proteins and enzymes for transcriptional initiation, such as NF-κB, Sp1, and TFIID, Tat has also been shown to bind a number of other factors which regulate chromatin structure located at the HIV promoter thus allowing access to the LTR DNA [9, 10, 18–27].
The basic building blocks of chromatin are organized into nucleosomes, each of which is made up of 146 bp of DNA wrapped around an octamer of histone proteins that consists of two copies of each of H2A, H2B, H3, and H4. The nucleosome can be divided into two domains, one of which is the structured histone-DNA and histone-histone globular domain, and the other is the highly basic N-terminal histone tails which contain multiple sites for post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and sumoylation [28–31]. The post-translational modifications present on each histone tail can direct higher order chromatin structure and consequently, transcription through a cycle of conflicting activation and repression signals [32–34]. Histone acetyltransferases (HATs), histone deacetylases (HDACs), kinases, and histone methyltransferases (HMTs) are all responsible for the addition/removal of covalent modifications on the histone tails [35–37]. In the case of retroviruses, the integration of proviral DNA into the genome of an infected cell requires the manipulation of cellular transcriptional machinery as well as cellular chromatin remodelers to accomplish proliferation, replication, and latent infection of the virus. Transcriptional silencing of the HIV-1 genome may be directly correlated with the state of chromatin packaging near the viral integration site [38–40].
Histone methyltransferases (HMTs) can methylate arginine residues such as 2, 8, 17, and 26 on H3 and residue 3 on H4. HMTs can also methylate specific lysine residues such as 4, 9, 27, 36, and 79 on H3 and residue 20 on H4 which serve as markers for the recruitment of chromatin organization complexes [41–43]. Specifically, lysine methylation is catalyzed by the SET-domain family of proteins which function to transfer a methyl group from S-adenosyl-L-methionine to the amino group of the lysine side chain, often on lysine 9 of H3 (H3-K9) . Historically, the methylation of H3-K9 has been linked to functionally repressed chromatin [33, 44, 45]. The selective methylation of H3-K9 results in the recruitment of the HP1 family of heterochromatic binding proteins therefore distinguishing transcriptionally silent chromatin regions [28, 33, 35, 44, 46–49]. The SET domain is comprised of approximately 130 amino acids surrounded by other domains which confer substrate specificity. The SUV39 family of SET-domain containing proteins, SUV39H1, SUV39H2, G9a, EHMT1, SETDB1, SETDB2, and SETMAR, specifically methylate lysines on Histone H3, however, more recent studies have also shown a preference for other proteins in addition to histones, therefore lending this family the name of protein lysine methyltransferases [41, 50, 51].
Lysine is a ~129 Da basic amino acid which is subject to multiple post-translational modifications such as acetylation, methylation, ubiquitination, and sumoylation. Lysine residues contain an ε-amino group which is highly catalytic for many metabolic and chemical reactions. Specifically, lysine residues can be mono-, di-, or trimethylated, each of which can differentially regulate chromatin structure and transcription. The chemical structure of lysine allows for only one type of post-translational modification to be present at any time, also allowing for steric hindrance of the modifications. This system of modification results in the need for both methylases and demethylases in response to particular cellular events. Of particular interest, while a lysine contains a methyl group, it cannot be simultaneously acetylated, therefore resulting in either an "on" or "off" orientation of the molecule. This consequence of the addition of a modification is important when regulating transcriptional activation or repression.
Tat itself is also subject to various post-translational modifications by host cellular proteins. Tat is phosphorylated, acetylated at lysines 28, 50, and 51, ubiquitinated at lysine 71, and methylated at arginine residues 52 and 53 [52–54]. Specifically, the basic domain (residues 49–57), which confers TAR RNA binding, is highly conserved and subject to acetylation on residues K28, K50, and K51 by CBP/p300, the result of which is crucial for Tat transactivation [55–59]. The acetylation of these residues is of great interest as a target for inhibition therapies; the prevention of acetylation would ensure only a low level of viral DNA is transcribed. Also, Tat retains its ability to dynamically shape the foundation of viral transcription through host machinery via its involvement with host cellular kinases. Recent studies have shown that Tat can be methylated by protein arginine methyltransferases (PRMTs) on arginine residues 52 and 53, resulting in a decreased interaction with TAR and cyclin T1 complex formation, therefore decreasing HIV-1 transcriptional activation [54, 60]. Here we investigated the methylation of lysine residues 50 and 51, which would compete with and therefore prevent the acetylation of the same residues and any subsequent viral transactivation. We especially were interested in these lines of investigation, since we had previously observed the presence of TIF-1α (a DNA-binding chromatin remodeling protein) when using proteomic analysis to identify cellular proteins bound to unmodified Tat . Here, we report the specific methylation of Tat lysine residues 50 and 51 by protein lysine methyltransferases. Initial screenings of the members of the SET-family for specific interactions with Tat in vitro revealed SETDB1/2 to be substrate specific for Tat. We observed that the H3-K9 methyltransferase SETDB1 can specifically methylate Tat preferentially at lysine 51. SiRNA knockdown studies of SETDB1 in transient transfected cells or cells with an integrated LTR reporter gene and associated cellular factors indicated an increase in LTR transactivation in the absence of the inhibitory modification. Collectively, our results imply that the modification of Tat at lysine 51 may contribute to an "on" or "off" phenotype of the HIV-1 promoter.
Lysine residue methylation of Tat by histone methyltransferases
The core histone tails have long been a primary example of the importance of post-translational modifications in transcriptional activation and repression. Histone modifications control the higher order chromatin structure and are facilitated by enzymes such as HATs, HDACs, and HMTs. Various combinations of modifications can be involved in the recruitment of specific transcription factors, therefore suggesting the "histone code" hypothesis. Many specific residues of the core histone tails have been identified as integral to transcriptional activation and repression and, consequently, their modifications have been documented. For instance, integral residues such as H3K9, H3K18, and H3K27 can be both acetylated and methylated, however, not simultaneously. Lysine methylation of histones is carried out by the SET-domain containing enzymes; therefore, this family of proteins was subjected to further investigation in the current manuscript.
Tat associates with SETDB1 and SETDB2 in vitro
As SETDB1 and SETDB2 were found to bind the unmodified Tat peptide, we next looked at the interaction with the full length wild type Tat protein. GST-bound Tat and Tax (control) proteins were allowed to incubate with whole cell extracts, and the associated complexes were probed for the presence of SETDB1 and SETDB2. SETDB1 was shown to associate with the full length Tat protein in greater abundance than SETDB2 (Figure 1D, Lane 3). The results of panels A-D are summarized in Figure 1E. Here, each enzyme utilized in our in vitro binding assay is depicted for their Tat binding affinity indicated on the right-hand side. SETDB1 and SETDB2 have the greatest affinity for wild type Tat, whereas, SUV39H1, SUV39H2, G9a, and SETMAR all bound to both unmodified and acetylated Tat to varying degrees. As SETDB1 had the highest affinity over SETDB2, this enzyme became the focus of further experimentation.
SETDB1 knockdown increases the transactivation of the viral LTR
We next performed a similar set of experiments in an LTR integrated system. TZM-bl cells are HeLa cells which contain both an integrated LTR-Luc reporter gene and an integrated LTR-β-Gal gene. To initiate viral transactivation, Tat must be transfected into these cells. We plated cells and allowed them to grow overnight before transfecting both Tat and the relevant siRNAs. We initially titrated Tat at 0.01, 0.1, and 1.0 ug to ensure that we could obtain an accurate standard curve for the luciferase readings (data not shown). Next, we transfected Tat into the cells at 0.1 ug, a suboptimal level, so that we could detect subtle differences in transcription activity resulting from the siRNA knockdowns. siGFP, siSETDB1, siTIF-1, and siG9a were all transfected along with Tat and 48 hours later cells were harvested for a luciferase assay. Figure 2B shows the results of the luciferase assay with the each value normalized to the siGFP control and activation represented in relative luciferase units. The knockdown of SETDB1 in these cells resulted in ~12 fold increase in activation as compared to the Tat control alone (lane 2). The knockdown of the other two proteins resulted in about ~6 fold increase in activation as compared to the Tat control. A confirmation western blot of the knockdown of SETDB1 and other proteins are shown on the bottom of panel B. Collectively, these results imply that reduced SETDB1 levels in a cell results in greater activation of the LTR.
Methylation of Tat at Lysines 50 and 51 by SETDB1 and their functional significance
We next asked whether methylation of Tat alters the specificity of cyclin T/TAR RNA binding in vitro. To do that, we used a biotin TAR pull-down RNA experiment and asked whether wild type or methylated Tat could still bring down cyclin T. Our initial set of experiments showed that when the reaction mixture contained TAR RNA (but not Poly-U RNA), wild type Tat, and purified Cdk9/cyclin T complex the affinity of cyclin T to TAR was fairly stable (data not shown). Next, we incubated purified methylated Tat 101 protein with TAR RNA and extract from CEM T-cells that contained endogenous Cdk9/cyclin T complexes. Following incubation and pull-down of TAR associated complexes, samples were separated on a 4–20% gel and Western blotted for the presence of cyclin T. Results, in Figure 3C showed that both unmodified Tat 86 or Tat 101 were able to bind to TAR RNA (lanes 1 and 2). However, methylated Tat was unable to form a Tat/cyclin T/TAR ternary complex in vitro (lane 3). Collectively, these results indicate that Tat methylation may decrease the affinity of Cdk9/cyclin T to the TAR RNA molecule.
Effect of siSETDB1 on HIV-1 reactivation
We have previously shown that acetylation of Tat lysine residues 50 and 51 results in an increase in transactivation of the LTR and promotes the incorporation of the Cdk9/cyclin T complex as well as other transcription factors into the active complex . As acetylation serves as an activation signal for Tat, it is safe to suggest that there is also a counter regulatory repression signal [63, 64]. Indeed, very recently Boulanger et al. and Xie et al. have shown that the methylation of Tat arginine residues 52 and 53 result in a decrease in association with viral transcription factors, as well as compromised transcriptional activation of the LTR [54, 60]. Here we propose that the methylation of Tat lysine 50 and 51 can result in a decrease in viral transcription.
The post-translational modifications observed on the histone tails can be easily correlated to modifications observed on other proteins. Commonly seen trends of modifications arise such as acetylation as a marker for activation (i.e. the transition from heterochromatin to euchromatin to initiate transcription) and methylation as a marker for repression (i.e. the addition of methyl groups to DNA to silence gene expression). Interestingly, the amino acid residues that can usually accept a post-translational modification are less frequent throughout a protein, but are also usually involved in key interactions, whether it can maintain the tertiary structure, enzymatic active sites, or binding sites for protein-protein interactions.
We show here that the lysine residues of Tat which are prone to acetylation, 50 and 51, can be preferentially methylated in vitro by the histone methyltransferase SETDB1. We show that the knockdown of this enzyme causes an increase in the transactivation of the viral LTR. The siRNA transfection experiments also included siRNAs against TIF1, G9a, and HP1. SETDB1 as a histone methyltransferase trimethylates H3K9, therefore initiating the formation of heterochromatin and gene silencing . This H3K9 methylation also serves as a mark for recruitment of the HP1 family of heterochromatin proteins . Therefore, it is possible that the methylation of Tat by SETDB1 could recruit HP1 and initiate transcriptional silencing through chromatin remodeling.
We have previously shown that Tat binds to a number of critical proteins including pCAF, Cyclin T1, and TIF-1 . TIF-1α is a member of the TRIM (tripartite motif) family of proteins. TRIM proteins contain the TRIM domain which is composed of three zinc-binding domains, a RING, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. The TRIM domain mediates protein-protein interactions  and oligomerization . TIF-1α has been demonstrated to be a repressor of RXR nuclear hormone receptors . TIF-1 (TRIM24) exhibits sequence similarities with the HIV restriction factor, TRIM5α, including the TRIM domain. It would be intriguing to find out if TIF-1 controls similar pathways as TRIM5α and could be a possible restriction factor for HIV-1 gene expression or control of methylation of nucleic acids. Possible reasoning for this is that TIF-1α has been shown to bind to HP1α, HP1β, TFIIE, Hsp70, PML, TAFII55, Zinc finger protein 10, RAR alpha, TAFII28, THR alpha 1, and other TIF-1 subunits.
siRNA mediated knock-down of various HMTs, including TIF-1 and SETDB1, indicated that decreased methyltransferase activity increased HIV LTR transcription in transient transfection assays. We also showed that the methylation of Tat by SETDB1 is preferential for both lysines 50 and 51. It is possible that any of these proteins is being mono-, di-, or tri- methylated by SETDB1 at any given time "on" or "off" of the HIV-1 LTR. Therefore, future experiments will determine the rate and type of Tat methylation on the LTR and in the presence of TAR RNA.
SiRNA and protein Reagents
Control and SETDB1, HP1-γ, TIF-1α, and G9a double stranded RNA oligonucleotides (siRNA) were purchased from Dharmacon Research (Lafayette, CO). Human SETDB1 and human SETDB2 proteins were expressed in baculovirus infected insect cells as amino-terminal fusion proteins with poly-histidine (H6) or H6-maltose binding protein (H6MBP). Baculovirus constructs were generated by Gateway recombinational cloning of cDNA clone, KG1T for SETDB1, (a generous gift from Dr. Greg Matera, Case Western Reserve University) and I.M.A.G.E. clone 5266911 for SETDB2 (Open Biosystems). Proteins were purified from soluble extracts by immobilized metal affinity chromatography (IMAC) using a nickel charged HisTrap-HP prepacked column (GE Healthcare) followed by anionic exchange using a HiTrap Q prepacked column (GE Healthcare) (H6MBP-SETDB1 only). Proteins were stored in buffer containing 20 mM Tris-HCl pH8.0, 50 mM NaCl, 10% glycerol, and 1 mM dithiothreitol at -80°C. Protein concentration was determined by Bradford assay (BioRad) relative to BSA.
Core human histones (all four) were purified from Hela cells and WT Tat 1–86 was overexpressed in an E. coli system followed by column purification . Anti-ESET(SETDB1) and anti-SUV39H1 antibodies were purchased from Cell Signaling (Danvers, MA). Anti-SETDB2 antibody was purchased from Abgent (San Diego, CA). Anti-SETMAR and anti-G9a antibodies were purchased from Abcam (Cambridge, MA). Tat WT and mutant peptides were synthesized and purchased commercially from SynBioSci (Livermore, CA) with the following sequences: Tat WT 45–54 (I-S-Y-G-R-K-K-R-R-Q), Tat K50A (I-S-Y-G-R-A-K-R-R-Q), Tat K51A (I-S-Y-G-R-K-A-R-R-Q), Tat K50, 51A (I-S-Y-G-R-A-A-R-R-Q). The purity of each peptide was analyzed by HPLC to greater than 98%. Mass spectral analysis was also performed to confirm the identity of each peptide as compared to the theoretical mass (Applied Biosystems Voyager System 1042). Peptides were resuspended in dH2O to a concentration of 1 mg/mL. Biotin-Tat and Biotin-Acetylated Tat were purified as published previously .
C8166 is an HTLV-1 infected T-cell line and TZM-bl is a cell line derived from HeLa cells containing Tat-inducible Luciferase and β-Gal reporter genes. C81 cells are grown in RPMI-1640 media containing 10% FBS, 1% L-glutamine, and 1% streptomycin/penicillin (Quality Biological). TZM-bl cells were grown in Dulbecco's modified Eagle's medium (DMEM) containing 10% FBS, 1% L-glutamine, and 1% streptomycin/penicillin (Quality Biological). All cells were incubated at 37°C and 5% CO2. Cells were cultured to confluency and pelleted at 4°C for 15 min at 3,000 rpm. The cell pellets were washed twice with 25 mL of phosphate buffered saline (PBS) with Ca2+ and Mg2+ (Quality Biological) and centrifuged once more. Cell pellets were resuspended in lysis buffer (50 mM Tris-HCl, pH 7.5, 120 mM NaCl, 5 mM EDTA, 0.5% NP-40, 50 mM NaF, 0.2 mM Na3VO4, 1 mM DTT, one complete protease cocktail tablet/50 mL) and incubated on ice for 20 min, with a gently vortexing every 5 min. Cell lysates were transferred to eppendorf tubes and were centrifuged at 10,000 rpm for 10 min. Supernatants were transferred to a fresh tube where protein concentrations were determined using Bio-Rad protein assay (Bio-Rad, Hercules, CA).
SETDB1-directed siRNA pool (ON-TARGET plus SMARTpool reagent L-020070-00), TIF-1α-directed siRNA pool (ON-TARGET plus SMARTpool reagent L-005387-00), HP1-γ-directed siRNA pool (ON-TARGET plus SMARTpool reagent L-010033-00) and G9a-directed siRNA pool (ON-TARGET plus SMARTpool reagent L-006937-00) were purchased from Dharmacon. TZM-bl cells were seeded in 6 well plates at 400,000 cells/well in DMEM containing 10% FBS. The following day, the cells were transfected with 0.01, 0.1, or 1.0 ug Tat plasmid and/or with either siGFP, siSETDB1, siTIF-1, siG9a, or siHP1-γ (Dharmacon) using Metafectene (Biontex) lipid reagent. Total amount of siRNA was held constant using siGFP. Cells were harvested forty-eight hours post transfection for protein concentration and luciferase readings.
Tat peptides (amino acids [aa] 42 to 52) were synthesized with a biotin tag on a PAL-polyethylene glycol-polystyrene resin by continuous flow solid-phase synthesis on a Perspective Biosystems Pioneer synthesizer (Framingham, MA) using HBTU-activated 9-fluorenylmethoxy carboxyl amino acids and were synthetically acetylated at positions 41/50/51 or 50/51, respectively . Synthesized Tat peptides (aa 36 to 53 and 42 to 54), labeled with biotin at the N terminus and with or without an acetyl group at lysines 50 and 51, were used in the pull-down assays. C81 whole cell extracts (2 mg) were prepared and incubated with biotin labeled Tat peptides (WT and acetylated, 1.0 ug) in TNE50 buffer (100 mM Tris-HCl, pH 7.5; 50 mM NaCl; 1 mM EDTA; 0.1% NP-40) overnight at 4°C. Streptavidin beads (Boehringer Mannheim) were added to the mixture and incubated for 2 h at 4°C. The beads were washed once with each TNE300, TNE150, and TNE50 + 0.1% NP-40. Bound proteins were separated on 4–20% SDS-PAGE gel and subjected to Western blotting with antibodies against SUV39H1, SUV39H2, G9a, SETDB1, SETDB2, and SETMAR.
C81 whole cell extracts (2 mg) were prepared and incubated with 10 ug of purified GST-Tat and GST-Tax constructs in TNE50 buffer (100 mM Tris-HCl, pH 7.5; 50 mM NaCl; 1 mM EDTA; 0.1% NP-40) overnight at 4°C. The following day, a 30% Protein A & G bead slurry (CalBioChem, La Jolla, CA) was added to each reaction tube and incubated for 2 hours at 4°C. Samples were spun and washed twice with TNE300 + 0.1% NP-40 (100 mM Tris, pH 8.0; 300 mM NaCl; 1 mM EDTA, 0.1% Nonidet P-40) and 1× with TNE50 + 0.1% NP-40 to remove nonspecifically bound proteins. Samples were loaded and run on a 4–20% Tris-Glycine SDS-PAGE gel and subjected to Western blotting with antibodies against ESET/SETDB1 and SETDB2.
TAR RNA Streptavidin bead pull-down assay
Purified biotin labeled TAR RNA (N terminus, 3 ug) or PolyU RNA were mixed with various purified proteins including wild type Tat 1–86 (0.5 ug), Tat mutant K50/51A (0.5 ug) or Baculovirus purified Cdk9/cyclin T (0.75 ug). Samples were incubated in TNE50 buffer (100 mM Tris-HCl, pH 7.5; 50 mM NaCl; 1 mM EDTA; 0.1% NP-40) with protease inhibitors and RNAsin overnight at 4°C. Streptavidin spharose beads (1/10 volume of a 30% slurry; Boehringer Mannheim) were added to the mixture and incubated for 2 h at 4°C. Bound proteins were separated on 4 to 20% sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE), and subjected to Western blotting with anti-cyclin T antibody.
GST-Tat 101 protein (2 mg) was first labeled in vitro with purified SETDB1 and S-Adenosyl-L-[methyl-3H] methionine. The reaction was incubated overnight at final volume of 35 ul. Also, 35 ul of sterile mineral oil was added to top of reaction to avoid evaporation of the reaction during the overnight incubation. The next day, 15 ul of 30% Glutathion beads were added for 2 hrs at 4°C and unbound material was washed with TNE50 + 0.1% NP-40. GST-Tat protein was eluted for 4 hrs at 37°C with reduced Glutathione. Purified methylated Tat was next incubated with CEM nuclear extract containing endogenous Cdk9/cyclin T complex (both active and inactive complex) at a final 2 mg/reaction. Biotin-TAR RNA at 1.5 ug was also added to the reaction mixture at the same time. Samples were incubated in TNE50 buffer with protease inhibitors and RNAsin overnight at 4°C. Subsequent reaction procedures were similar to what was described above.
In vitromethyltransferase and Filter Binding Assay
Full length WT Tat (3 ug), Tat peptides (2 ug), Tat mutant peptides (2 ug), histone H3 mutant peptide (2 ug, K to A mutations at residues 4, 9, 14, 18, 23, 27, 36, and 37) and core histones (1 ug) were incubated with 2 μg of purified enzyme (SETDB1, SETDB2) in the presence of 0.55 μCi S-Adenosyl-L-[methyl-3H] methionine (GE Healthcare, Piscataway, NJ) and reaction buffer (50 mM Tris-HCl, pH 8.5, 20 mM KCl, 10 mM MgCl2, 250 mM sucrose, 10 μM β-mercaptoethanol) overnight at 37°C in a final reaction volume of 30 μl. The overnight methylation reactions were spun briefly and spotted on GF/C membranes (Millipore) in duplicate and allowed to dry. The filters were washed three times in excess cold 10% TCA, 1% sodium phosphate followed by once with 100% ethanol. The filters were allowed to dry and counted in Beckman Coulter LS6001C scintillation counter in scintillation fluid.
Transfection of HLM-1 cells
Log phase HLM-1 cells (5 × 106/sample) were electroporated (210 volts, 800 mA) with siSETDB1 and siHP1 and incubated in complete media for 48 hrs. Cells were subsequently washed and treated with tyrpsin for 2 min. Next, cells were washed and incubated with Tat (10 ug) in Tat buffer (PBS + 0.01 mM DTT) for 4 hrs at 37°C in RPMI without serum. Cells were then plated in complete media for 6 days at 37°C and supernatants were process for RT activity.
Forty-eight hours post transfection, luciferase activity of the firefly luciferase of the TZM-bl cells was measured with the DualGlo Luciferase Assay (Promega). Luminescence was read from a 96 well plate on an EG&G Berthold luminometer. LTR driven firefly luciferase levels were normalized to siGFP levels. Data shown represents at least two repeats of each condition.
Plasmids (LTR-CAT or CMV-Tat) were transfected by electroporation using a Bio-Rad Gene Pulser (Bio-Rad, Richmond, CA) at 960 μF and 230 Volts. After 48 h, cells were lysed and chloramphenicol acetyltransferase (CAT) and luciferase activities were determined. Luciferase was measured using the Luciferase assay system (Promega). For the CAT assay, a standard reaction was performed by adding the cofactor acetyl coenzyme A to a microcentrifuge tube containing cell extract (50 ug) and radiolabeled (14C) chloramphenicol in a final volume of 50 μl and incubating the mixture at 37°C for 1 h. The reaction mixture was then extracted with ethyl acetate and separated by thin-later chromatography on silica gel plates (Baker-flex silica gel thin-later chromatography plates) in a chloroform-methanol (19:1) solvent. The resolved reaction products were then detected by exposing the plate to a PhosphorImager cassette.
This work was supported by grants from the George Washington University REF funds to FK, and Akos Vertes, a grant from Conrad and by an NIH grant AI071903-01 to FK. This research was also supported in part by the Intramural Research Program of the Center for Cancer Research, National Cancer Institute, NIH. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U. S. Government. Finally, the authors would like to acknowledge Dr. Monsef Benkirane for valuable reagents including various HP-1 siRNAs and Tat 101 construct and proteins.
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