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  • Oral presentation
  • Open Access

Identification of human monoclonal antibodies specific for CCR5 from an antibody library derived from HIV-infected long-term non-progressors

  • 1, 2,
  • 1,
  • 3,
  • 1,
  • 1,
  • 3 and
  • 1
Retrovirology20063 (Suppl 1) :S61

https://doi.org/10.1186/1742-4690-3-S1-S61

  • Published:

Keywords

  • Neutralize Antibody
  • Human Monoclonal Antibody
  • Slow Progression
  • Positive Individual
  • Antibody Library

It has been reported that about one quarter of long-term nonprogressors had anti-CCR5 antibodies and such antibodies were not found in disease progressing HIV-1 positive individuals [1]. To identify such antibodies we panned an HIV immune library constructed from bone marrow of three long-term nonprogressors against a synthetic sulfated N-terminal CCR5 peptide (2–15) (R5Nt). Twenty anti-CCR5 monoclonal antibodies were selected that bind to the R5Nt and cell-associated CCR5. Sequence analysis revealed that eighteen clones have VHs that were derived from the same germline sequence. Preliminary data showed that these antibodies inhibited primary HIV-1 isolates from clade B and E as tested in a pseudovirus assay. Identification of these anti-CCR5 human monoclonal antibodies indicates possible contribution to mechanisms determining the slow progression or lack of disease in long-term nonprogresssors. The selected anti-CCR5 antibodies may have potentials as therapeutics and/or prophylactics in combination with other HIV-1 neutralizing antibodies and antiretroviral drugs.

Authors’ Affiliations

(1)
Center for Cancer Research Nanobiology Program, National Cancer Institute-Frederick, National Institutes of Health, Frederick, Maryland 21702, USA
(2)
SAIC-Frederick, Inc., National Cancer Institute-Frederick, National Institutes of Health, Frederick, Maryland 21702, USA
(3)
Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA

References

  1. Pastori C, Weiser B, Barassi C: Long-lasting CCR5 internalization by antibodies in a subset of long-term nonprogressors: a possible protective effect against disease progression. Blood. 107: 4825-4833. 10.1182/blood-2005-06-2463.Google Scholar

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