Figure 2
From: Determination of the relative amounts of Gag and Pol proteins in foamy virus particles
Immunoblot of a dilution series of recombinant Gag and Pol proteins, a cellular lysate (C), and extra-cellular virus (V) detected with the MABs SGG1 (Gag), 15E10 (RT), and 3E11 (IN). (C) was obtained by harvesting lytically infected BHK-21 cells, and (V) prepared by concentrating the supernatant of lytically infected cells through a sucrose cushion. On the right side the indicated amounts of recombinant proteins, specifying FV Gag and Pol proteins as shown in Fig. 1, were mixed and loaded onto an SDS-PAGE.