Viral protein R of human immunodeficiency virus type-1 induces retrotransposition of long interspersed element-1
- Kenta Iijima†1,
- Noriyuki Okudaira†1, 2, 11,
- Masato Tamura1,
- Akihiro Doi1, 2,
- Yoshikazu Saito1,
- Mari Shimura1,
- Motohito Goto3,
- Akihiro Matsunaga1,
- Yuki I Kawamura4,
- Takeshi Otsubo4,
- Taeko Dohi4,
- Shigeki Hoshino1,
- Shigeyuki Kano2, 5,
- Shotaro Hagiwara6,
- Junko Tanuma7,
- Hiroyuki Gatanaga7,
- Masanori Baba8,
- Taku Iguchi9, 12,
- Motoko Yanagita9,
- Shinichi Oka7,
- Tadashi Okamura3, 10 and
- Yukihito Ishizaka1Email author
© Iijima et al.; licensee BioMed Central Ltd. 2013
Received: 16 November 2012
Accepted: 18 July 2013
Published: 5 August 2013
Viral protein R (Vpr), a protein of human immunodeficiency virus type-1 (HIV-1) with various biological functions, was shown to be present in the blood of HIV-1-positive patients. However, it remained unclear whether circulating Vpr in patients’ blood is biologically active. Here, we examined the activity of blood Vpr using an assay system by which retrotransposition of long interspersed element-1 (L1-RTP) was detected. We also investigated the in vivo effects of recombinant Vpr (rVpr) by administrating it to transgenic mice harboring human L1 as a transgene (hL1-Tg mice). Based on our data, we discuss the involvement of blood Vpr in the clinical symptoms of acquired immunodeficiency syndrome (AIDS).
We first discovered that rVpr was active in induction of L1-RTP. Biochemical analyses revealed that rVpr-induced L1-RTP depended on the aryl hydrocarbon receptor, mitogen-activated protein kinases, and CCAAT/enhancer-binding protein β. By using a sensitive L1-RTP assay system, we showed that 6 of the 15 blood samples from HIV-1 patients examined were positive for induction of L1-RTP. Of note, the L1-RTP-inducing activity was blocked by a monoclonal antibody specific for Vpr. Moreover, L1-RTP was reproducibly induced in various organs, including the kidney, when rVpr was administered to hL1-Tg mice.
Blood Vpr is biologically active, suggesting that its monitoring is worthwhile for clarification of the roles of Vpr in the pathogenesis of AIDS. This is the first report to demonstrate a soluble factor in patients’ blood active for L1-RTP activity, and implies the involvement of L1-RTP in the development of human diseases.
Viral protein R (Vpr), an accessory gene of human immunodeficiency virus type-1 (HIV-1), encodes a virion-associated nuclear protein of ~15 kDa . Vpr has a variety of biological functions, including cell cycle abnormalities at the G2/M phase and apoptosis of T cells and neuronal cells (for a recent review, see ref. ). Notably, it was shown that Vpr was present in the blood of HIV-1-positive patients , and we previously reported that 20 of 52 blood samples from HIV-1-positive patients examined were positive for Vpr . Blood Vpr was detected in patients with high titres of HIV-1 and, interestingly, was also detected in patients with low viral titres . On the other hand, purified recombinant Vpr protein (rVpr) functions as a trans-acting factor [5, 6], and rVpr activated viral replication in latently infected cells by increasing production of interleukin-6 (IL-6) by monocytes . Further analyses revealed that rVpr-induced IL-6 production depended on p38, a mitogen-activated protein kinase (MAPK), and CCAAT/enhancer-binding protein β (C/EBP-β) . These observations suggest that blood Vpr could induce various clinical symptoms, but it remained unclear whether blood Vpr is biologically active.
Long interspersed element-1 (LINE-1, L1) and Alu are major endogenous retroelements, accounting for ~17 and ~10% of the human genome, respectively [8, 9]. As an autonomous retroelement, L1 can retrotranspose not only itself but also other retroelements, such as Alu and SVA (short interspersed element-variable number tandem repeat-Alu, SINE-VNTR-Alu). Intriguingly, a single human cell contains more than 5 × 105 copies of L1, 80–100 of which are competent for retrotransposition (L1-RTP) . During early embryogenesis, L1-RTP incidentally disrupts gene structures, leading to the development of inborn errors [11, 12]. Of note, approximately 100 types of inheritable diseases have been identified as sporadic cases caused by mutagenic RTP of L1 or Alu . Although most studies of L1-RTP have focused on early embryogenesis [13–16], recent lines of evidence suggest that L1-RTP is also induced in somatic cells [17–20]. In tumors of epithelial-cell origins and hepatomas, de novo L1 insertions were detected in the vicinity of tumor suppressor genes, suggesting that L1-RTP is actively involved in carcinogenesis [21, 22]. Because L1-RTP alters cellular properties by causing various genetic alternations, including gene deletions [23, 24], DNA damage , apoptosis  and immune responses , deregulation of L1-RTP in somatic cells likely functions as a trigger of various diseases.
Here we present evidence that Vpr is active for induction of L1-RTP, and further demonstrate that 6 of 15 blood samples from HIV-1 patients were positive for Vpr-induced L1-RTP. Interestingly, rVpr reproducibly induced L1-RTP in various organs, including the kidney, when administered to mice that harbored human L1 as a transgene (hL1-Tg mice) [28, 29]. Clinically, HIV-1-associated nephropathy (HIVAN), which is mainly observed among African-Americans , is an end-stage renal deficiency that is found without apparent correlation with the viral load [31, 32]. In view of reports that Vpr is a candidate molecule responsible for HIVAN [33, 34], we propose that monitoring blood levels of Vpr is important for determining its involvement in the pathogenesis of HIVAN.
rVpr induces L1-RTP
rVpr induces L1-RTP in vivo
We also investigated the methylation status of CpG in the L1-5′UTR in the rVpr-treated kidney. Analysis by the COBRA method , a method of quantifying CpG methylation, detected no apparent changes in the methylation status of CpG before or after six administrations of 10 ng of rVpr (Additional file 8: Figure S6).
rVpr-induced L1-RTP depends on an AhR-p38-C/EBP-β cellular cascade
To determine the importance of the LxxLL motif of Vpr for the induction of L1-RTP, we investigated the activity of a Vpr mutant containing AQQAA instead of LQQLL (LA mutant, “LAM” in Figure 5). First, studies of forced expression of wild-type Vpr (WT Vpr) and the LA mutant revealed that the mutant was not active for induction of L1-RTP (Figure 5E, left panel), although comparable levels of each protein were detected (Figure 5E, right panel). Additionally, IP-WB analysis detected an association between WT-Vpr and AhR (Figure 5F, lane 5), but less interaction of the LA mutant with AhR (Figure 5F, lane 6). These data suggest that Vpr-induced L1-RTP is dependent on a molecular interaction with AhR via the LxxLL motif of Vpr.
In a previous study, we showed that p38 and C/EBP-β are important for understanding the cellular response to exogenously applied rVpr , implying that these molecules are also involved in the induction of L1-RTP by rVpr. To confirm this, we focused on the effect of C/EBP-β siRNA on rVpr activity. As shown in Figure 6B, transfection of the C/EBP-β siRNA down-regulated the endogenous protein level and attenuated rVpr-induced L1-RTP (Figure 6C, lane 9; see also Additional file 10: Figure S8E for data obtained using another siRNA targeting C/EBP-β mRNA, which was used in the experiment shown in Figure 6D). In contrast, siRNAs against CREB and c-Jun did not attenuate rVpr-induced L1-RTP (Figure 6D), although each siRNA efficiently down-regulated endogenous protein expression (Additional file 10: Figure S8F and G). One possible reason is that MAPK inhibitors are not specific for target molecules .
Chromatin recruitment of ORF1 induced by rVpr is dependent on AhR
Previously, we reported that FICZ is a potent activator of L1-RTP, and that its activity was dependent on ARNT1, but not on AhR . To determine the functional link between ORF1 and ARNT1, we performed IP-WB analysis after transfecting pORF1-EGFP into HuH-7 cells. ORF1 was detected in an extract of cells treated with FICZ and was recovered using α-ARNT1 (Figure 7D, upper panel, lane 10). By contrast, it was not recovered from extracts of cells treated with rVpr using α-ARNT1 (Figure 7D, upper panel, lane 12). Consistent results were obtained in a reverse experiment, in which WB using α-ARNT1 was performed on a sample recovered by IP using α-EGFP (Figure 7D, lower panel). In this case, the cell extract obtained after FICZ treatment yielded a positive signal (Figure 7D, lower panel, lane 10). These data suggest that the association between ORF1 and ARNT1 is induced by exogenous FICZ but not rVpr.
rVpr-induced L1-RTP depends on an AhR-p38-C/EBP-β cellular cascade
Here we found that Vpr is a viral protein active for the induction of L1-RTP. Experiments using MNF, siRNAs targeting AhR and C/EBP-β mRNAs, and MAPK inhibitors revealed that rVpr-induced L1-RTP depends on an AhR-p38-C/EBP-β cellular cascade (Figures 5 and 6). We confirmed by in vitro experiments that rVpr did not increase expression of L1 mRNA or the splicing efficiency of an immature EGFP transcript derived from the reporter L1 construct (Additional file 15: Figure S13). Moreover, no apparent changes in the CpG methylation status were observed in the 5′UTR of the exogenous hL1 gene in the kidneys of hL1-Tg mice that had been treated with rVpr (Additional file 8: Figure S6). Our data suggest that rVpr-induced L1-RTP is controlled at the post-transcriptional level, although it has been proposed that L1-RTP is influenced at the transcriptional level by the methylation status of the L1-5′UTR [53, 54].
In addition to the LA mutant, we investigated the activity of a Vpr mutant lacking the C-terminal 12 amino acids (ΔC12). A PCR-based assay revealed that the ΔC12 mutant was not active for the induction of L1-RTP (Additional file 16: Figure S14). It has been shown that Vpr has an affinity for nucleic acids, which is attributable to the basic moiety in the C-terminal region of Vpr . To exclude the possibility that the induction of AhR-dependent L1-RTP by Vpr depends on binding to nucleic acids, we investigated the interaction between Vpr and AhR after nuclease treatment. IP-WB analysis combined with treatment with benzonase, a nuclease that degrades both DNA and RNA, revealed that their interaction was not reduced (Additional file 17: Figure S15A). Additionally, ORF1 and AhR constitutively formed a complex, and their interaction was also resistant to nuclease treatment (Additional file 17: Figure S15B). Moreover, rVpr triggered chromatin recruitment of ORF1 in an AhR-dependent manner (Figure 7B). Taken together, these data suggest that Vpr functions as an AhR ligand, and activates a cellular cascade for the induction of L1-RTP.
Biologically active Vpr is present in the blood of HIV-1-positive patients
We detected L1-RTP-inducing activity in the blood of HIV-1 patients: 6 of 15 patients were positive for the induction of L1-RTP (Figure 2A). The L1-RTP activity in those six patients was selectively blocked by 8D1, a mAb against Vpr (Figure 2B). Interestingly, we previously examined blood Vpr by IP-WB analysis, and detected Vpr in 20 of 52 blood samples from HIV-1 patients . Interestingly, the positive frequencies observed in these two sets of experiments are comparable, but greater numbers of samples are needed to conclude that blood Vpr is exclusively biologically active. Although it was reported that an antibody against Vpr is present in patients’ blood , and implied that Vpr activity would be blocked by such autoantibodies, our current experiments proved that blood Vpr is active for the induction of L1-RTP. Because L1-RTP can alter cellular properties by inducing DNA damage and apoptosis , it is tempting to speculate that blood Vpr can modify clinical outcomes of AIDS patients.
Consistent with our previous observation that Vpr protein was detectable in blood samples from HIV-1-positive patients with low viral titres , we here detected Vpr-induced L1-RTP in samples from patients with low viral titres. As shown in Figure 2, L1-RTP-inducing activity was detected in some blood samples, and IP-WB analysis successfully detected a Vpr signal (no. 15) (Additional file 4: Table S1 and Additional file 5: Figure S4). Intriguingly, however, the viral titre of patient 15 was 140 copies/mL (Figure 2D, closed diamond and Additional file 4: Table S1). By striking contrast, the viral titres of the blood samples from patients 3, 8 and 4 were >104 copies/mL, but no apparent L1-RTP-inducing activity was detected. Although it remains completely unknown why Vpr was present in patients with low viral titres, one possible explanation would be that Vpr is secreted into the blood from latent foci in patients. In vitro experiments support the notion that Vpr is excreted by infected cells and functions as a soluble protein with bystander effects .
rVpr is active for the induction for L1-RTP in vivo
Repeated intravenous administration of 10 ng of rVpr, a dose comparable to patients’ blood levels , induced L1-RTP in vivo (Figure 4B). We observed that administration of rVpr induced L1-RTP in various organs, such as the lymph nodes and spleen. Additionally, we found that Vpr also induced L1-RTP in the kidney (Figure 4A and Additional file 6: Table S2). Notably, even a single injection of 250 ng of rVpr into the tail vein induced L1-RTP in the kidneys (Figure 4A, right panels), suggesting that the kidney is a target organ of Vpr-induced L1-RTP. Immunohistochemical analysis showed that Vpr induced L1-RTP in RTECs, especially in proximal RTECs (Figure 4D). Previously, it was reported that Vpr and Nef are candidate mediators of HIVAN: forced expression of these viral genes in mouse podocytes resulted in proteinuria and glomerular diseases . Although it was proposed that renal dysfunction is a direct effect of primary HIV-1 infection in RTECs , it remains to be investigated whether repeated administration of rVpr causes renal insufficiency.
HIVAN develops mostly in people of African descent, and shows the strong influence of genetic traits [59–61]. However, its mechanism remained completely unknown. Importantly, HIVAN has no apparent correlation to viral load , and, intriguingly, it has been proposed that the kidneys are a latent reservoir of HIV-1 [62, 63]. Based on these observations, it is plausible that both blood-circulating Vpr and Vpr secreted locally from a latent reservoir (the kidney, for example) attack RTECs. To prove this, further studies are required to determine whether the kidney is an organ from which Vpr is constitutively secreted.
In addition to their clinical relevance for HIV-1 pathogenesis, our findings should have a general impact on the involvement of L1-RTP in human diseases. By analysis of tumors using second-generation sequencing technology, de novo L1 insertions were detected in the vicinity of tumor suppressor genes [21, 22], suggesting that L1 insertion was actively involved in carcinogenesis. Additionally, it was shown that de-regulation of L1-RTP is positively linked to the development of autoimmune diseases . Although these lines of evidence revealed that L1-RTP is induced in somatic cells and is involved in the development of human diseases, it remained unclear how L1-RTP is induced in somatic cells. It was previously reported that 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), a food-borne carcinogen, induced L1-RTP in the mouse mammary gland, a target organ of carcinogenesis, when it was administered orally to hL1-Tg mice . Given that PhIP is present in broiled meat  and has been detected in human breast milk , it is plausible that humans are susceptible to the induction of L1-RTP by environmental factors. Further study is required to demonstrate the activity of L1-RTP under pathological conditions, enabling the roles of L1-RTP in disease development to be specified.
Six of the 15 blood samples from HIV-1-positive patients examined were positive for Vpr-induced L1-RTP. L1-RTP-inducing activity was detected in blood samples with low viral titres. Monitoring circulating Vpr in relation to clinical outcomes is important to clarify the roles of Vpr in AIDS symptoms. The present study is the first to show that L1-RTP-inducing activity is present in vivo, shedding light on the possible involvement of L1-RTP in human diseases. In further research, it will be important to detect L1-RTP-inducing activity under pathological conditions.
Chemicals and cells
HuH-7 cells (RCB1366) and HEK293T cells (RCB2202) were obtained from the Riken BioResource Centre Cell Bank. They were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal calf serum (Sigma-Aldrich, St. Louis, MI, USA). The transfection efficiencies were ~70 and ~30%, respectively, as determined by fluorescence-activated cell sorting (FACS) on day 2 after transfection of plasmid DNA encoding enhanced green fluorescent protein (EGFP) (data not shown). MNF was kindly provided by Dr. Gabriele Vielhaber (Symrise, Holzminden, Germany). SB20358, SP60012, PD98059 and lipopolysaccharide (L8274) were from Sigma-Aldrich. FICZ was obtained from Enzo Life Sciences (Plymouth Meeting, PA, USA). Protease inhibitors (Roche Diagnostics, Tokyo, Japan) were also purchased.
Antibodies against AhR, (Santa Cruz Biotechnology, Santa Cruz, CA, USA), ARNT1 (Santa Cruz Biotechnology), β-tubulin (Thermo Fisher Scientific, Waltham, MA, USA), H2AX (Millipore, Billerica, MA, USA), C/EBP-β (Cell Signaling Technology Inc., Danvers, MA, USA), FLAG (Sigma-Aldrich), EGFP (rabbit antibody: Medical & Biological Laboratories, Co., Ltd., Nagoya, Japan; mouse monoclonal antibody: Abcam, Cambridge, United Kingdom), aquaporin 1 (AQP1; Abcam) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Trevigen, Gaithersburg, MD, USA) were used as the primary antibodies. A rabbit polyclonal antibody against human ORF1 was generated using the peptide MGKKQNRKTGNSKTQSAC (amino acids denoted by single letters) as an immunogen (Medical & Biological Laboratories). An Alexa Fluor 546-conjugated antibody to phalloidin (Invitrogen, Carlsbad, CA, USA) was purchased. As secondary antibodies, α-mouse IgG (GE Healthcare Bio-Sciences Corp., Piscataway, NJ, USA), α-rabbit IgG (GE Healthcare), and α-goat IgG (Santa Cruz Biotechnology), all of which were conjugated with horseradish peroxidase, were used. For immunohistochemical analysis, Alexa Fluor 555-conjugated goat α-rabbit IgG (Invitrogen) and Alexa Fluor 488 goat α-mouse IgG (Invitrogen) were used as the secondary antibodies. Hoechst 33258 was purchased from Invitrogen.
Based on recent reports that RTIs efficiently blocked L1-RTP [39, 40], we used 4′-Ed4T, which has more potent inhibitory activity than d4T and less effect on DNA polymerases, and which is currently undergoing phase IIb clinical trials in HIV-1-infected patients . Two hours before injection of rVpr, 50 μmoles of 4′-Ed4T was injected intraperitoneally to give a final concentration of approximately 25 μM when most of the compound is transferred to the blood, the estimated volume of which is ~2 mL.
Purification of rVpr and assays of L1-RTP
rVpr was expressed using pGEX-6P-1 in Escherichia coli BL21 and purified as described previously (Figure 1A) . Purified rVpr was tested for endotoxin using a highly sensitive lipopolysaccharide (LPS) assay with Limulus amebocyte lysate, the detection limit of which was 0.25 EU/mL (Wako Pure Chemical Ind., Ltd., Osaka, Japan). For L1-RTP assays, we used two reporter constructs, pEF06R  and pCEP4/L1mneoI/ColE1 (pL1-NeoR) [28, 35–37], for semi-quantitative and quantitative PCR, and colony formation assays, respectively. Each construct contained all components of human L1 with single transcriptional units with EGFP or NeoR, which were inserted in reverse orientation. When L1-RTP occurs, the intron within each reporter gene is spliced out, and then pEF06R expresses functional EGFP, whereas pL1-NeoR expresses a functional neomycin-resistance gene (NeoR). Cells were transfected with pEF06R or pL1-NeoR using Lipofectamine 2000 (Invitrogen) or Xfect (Takara Bio Inc., Shiga, Japan). Cells were selected for 2 days with puromycin (Puro, 0.5 μg/mL) for pEF06R, or with hygromycin (Hygro, 25 μg/mL) for pL1-NeoR. Next, cells were treated for additional 2 or 3 days with the indicated amounts of rVpr.
For the PCR assay, genomic DNA was prepared from harvested cells using a DNA extraction system (QuickGene; Fujifilm, Tokyo, Japan). For semi-quantitative PCR, primers that were designed for each exon would amplify a product of ~1040 bp, whereas they would generate a product of ~140 bp upon L1-RTP. Thus, occurrence of L1-RTP was determined by evaluating the size of the amplified product [28, 29, 37]. After staining of amplified DNA with SYBR Green I (LONZA, Basel, Switzerland), signal intensities of the 140 bp bands were measured using a molecular imager (FX-PRO; Bio-Rad, Hercules, CA, USA) and normalized by the signal intensity of the β-actin band, used as the internal control. Relative intensities (RIs) of each 140 bp band were calculated by standardizing the signal of the buffer-treated sample as “1”.
For qPCR analysis, 5′-GAA CGG CAT CAA GGT GAA CT-3′ and 5′-GGG GTG TTC TGC TGG TAG TG-3′, which were designed for each exon of the EGFP gene, were used as forward and reverse primers, respectively. A TaqMan-probe (5′-FAM- TGC AG * C TGG CCG AC -MGB-3′) (Invitrogen) was used to detect an amplicon of 87 bp in length (* denotes the exon junction). Template DNA was amplified with Eagle Taq Master Mix (Roche Diagnostics) and a CFX Connect Real-Time System (Bio-Rad) using the following amplification conditions: 95°C for 10 min, followed by 45 cycles of 95°C for 15 sec and 64°C for 15 sec. To obtain a standard curve for EGFP-qPCR, EGFP DNA generated after the induction of L1-RTP was amplified using the above primers and cloned into the pGEM-T Easy vector (Promega, Madison, USA). After confirmation of the nucleotide sequence, standard samples were prepared by mixing human or mouse genomic DNA with the EGFP-containing plasmid to give 1.0, 10-1, 10-2, 10-3 and 10-4 copies/cell. To normalize the amounts of input DNA, human β-globin or mouse β-actin was quantified by qPCR with SYBR Premix Ex Taq (TaKaRa) and the CFX Connect Real-Time System (Bio-Rad). For human β-globin, the forward primer was 5′-TTG GAC CCA GAG GTT CTT TG-3′ and the reverse primer was 5′-GAG CCA GGC CAT CAC TAA AG-3′; for mouse β-actin, the forward primer was 5′-TGA CGT TGA CAT CCG TAA AGA CC-3′ and the reverse primer was 5′-AAG GGT GTA AAA CGC AGC TCA-3′.
In the colony formation assay, ~2.0 × 106 cells were transfected with pL1-NeoR and selected with 25 μg/mL Hygro, and 1.0 × 105 cells were re-plated to new plates (Split). Next, cells were treated for 2 days with rVpr and further cultured in the presence of neomycin (800 μg/mL) [35–37]. In the initial experiment, we used 5–10 ng/mL rVpr because the maximum reported plasma Vpr concentration in HIV-1-positive patients is ~5 ng/mL . To determine Vpr activity for L1-RTP induction, each of the six plates was treated with rVpr or a buffer control for 2 days, and further cultured in the presence of neomycin. After 3–4 weeks, cell aggregates were stained with methylene blue, and colonies were enumerated. To minimize plate-to-plate variation, the colony numbers of the middle four of the six plates were subjected to statistical analysis. At least two independent experiments were performed, representative results of which are shown.
Suppression of rVpr-induced L1-RTP by mAbs against Vpr
The effects of mAbs against Vpr on the induction of L1-RTP were investigated by applying 5 ng of rVpr with 500 ng of 8D1 and C217 , giving an approximately 10-fold excess molar amount of rVpr. After 60 min of incubation at room temperature, a 300 μL reaction mixture was filtrated and added to 1.5 mL of culture medium of cells. As a control, a SARS mAb, an irrelevant mAb that recognizes a spike protein of the severe acute respiratory syndrome corona virus (SARS-CoV), was used.
Effect of down-regulation of endogenous proteins on induction of L1-RTP
For each gene, two small interfering RNAs (siRNAs) were prepared (Applied Biosystems, Foster City, CA, USA or Thermo Scientific), and their functions were evaluated by transfection into cells followed by WB analysis. The nucleotide sequences of each siRNA are shown in Additional file 18: Table S3. To evaluate the inhibitory effects of the siRNAs on L1-RTP induction, each siRNA was introduced on day 3 after initial transfection with pL1-NeoR or pEF06R. Two days later, the cells were re-plated, incubated for 2 days with rVpr, and subjected to analysis. Silencer Negative Control siRNAs (cat. no. AM4613, AM4637 and AM464; Life Technologies Corporation, Carlsbad, CA, USA) were used as controls.
Effects of MAP kinase inhibitors on rVpr-induced L1-RTP
HuH-7 cells were transfected with pEF06R and selected for 2 days with 0.5 μg/mL Puro. On day 3 after transfection, cells were re-plated and subjected to an L1-RTP assay. To examine the effects of MAPK inhibitors, SB202190, SP600125 and PD98059 at concentrations of 1, 100 and 20 μM, respectively, were added 1 h before the addition of rVpr. The cells were exposed to 10 ng/mL rVpr for 3 days and subjected to qPCR analysis. Genomic DNA was isolated using the QuickGene DNA Tissue Kit S and QuickGene-800 (Fujifilm). To selectively detect EGFP genes derived from L1-RTP, ~250 ng of DNA were used as the qPCR template. To amplify β-globin gene as an internal control, ~50 ng of DNA were used as the qPCR template.
Administration of rVpr to hL1-Tg mice and L1-RTP assessment
For in vivo experiments, we used two transgenic mouse lines, #4 and #67, in which the L1-DNA fragment of pEF06R had been introduced as a transgene (hL1-Tg mice; Figure 1A, sidebar) [28, 29]. These two lines were selected because they display low background L1-RTP during embryogenesis but respond vigorously to environmental compounds . The CpG island of the 5′ untranslated region of introduced human L1 (L1-5′UTR) was highly methylated in #4 and #67 mice, as assessed by a PCR-based assay using methylation-specific primers . All animal experiments were approved by the Animal Care and Use Committee at the National Center for Global Health and Medicine (NCGM).
Fifteen blood samples obtained from anti-retroviral therapy-naïve male patients who presented to the NCGM hospital between October 1996 and October 2003 were subjected to the PCR-based assay. The patients were 21–44 years of age with viral loads and CD4 counts of 50–230,000 copies/mL and 315–795 cells/mL, respectively. Nine healthy volunteers served as controls. To detect L1-RTP-inducing activity, HEK293T cells were first transfected with pEF06R and selected with 0.5 μg/mL Puro. Then, 150 μL of each heat-inactivated patient serum sample was added to 1.35 mL of culture medium of HEK293T cells. To show that L1-RTP activity in patients’ blood was attributable to Vpr, 100 μL of serum was reacted for 60 min with 500 ng of 8D1 or SARS-S mAb at room temperature in a 300 μL reaction volume. The experimental protocol was approved by the institutional review board of NCGM.
L1-RTP activity of the Vpr mutant
The LA mutant, which contains AQQAA at codons 64–68, and wild-type (WT) Vpr were expressed as FLAG-tagged proteins using the pFLAG-CMV2 expression vector (Sigma-Aldrich). To obtain comparable levels of expression of each protein, the molar ratio of 1:4 of plasmid DNA for the wild-type Vpr and the LA mutant were transfected respectively. On the next day of transfection, cells were subjected to the PCR-based assay.
Chromatin recruitment of ORF1 induced by rVpr
We used the pORF1-TAP (tandem affinity purification) construct , which encodes a chimeric protein of ORF1, protein A and calmodulin-binding protein. On day 2 after transfection of pORF1-TAP into HuH-7 cells, 5 ng/mL rVpr was added to the culture medium, and cell extracts were prepared on the following day. The chromatin-enriched fraction (chromatin fraction) was isolated using a Subcellular Protein Fractionation Kit (Thermo Fisher Scientific) with micrococcal nuclease, as described previously . Detection of ORF1-TAP was performed by probing with a horseradish peroxidase-conjugated human IgG (Jackson ImmunoResearch West Grove, PA, USA). H2AX was used as an internal control for the chromatin fraction.
ORF1, AhR, and Vpr complex formation
HuH-7 or HEK293T cells were transfected with the plasmid constructs pFLAG-Vpr-Wt or pFLAG-Vpr-LA mutant, pORF1-EGFP and pFLAG-EGFP, which encode FLAG-tagged Vpr, a chimeric protein of ORF1 and EGFP, and FLAG-tagged EGFP, respectively. On day 2 after transfection, cells were treated with 10 ng/mL rVpr for 1 day to evaluate the dependence of the protein-protein interaction on Vpr. Then, cells were subjected to IP-WB analysis. To analyze the ORF1-AhR association, cells were suspended in a buffer containing 50 mM Tris (pH 7.5), 150 mM NaCl, 1% NP40, 1 mM EDTA and a protease inhibitor cocktail and subjected to brief sonication. For analysis of the Vpr-AhR association, cells were suspended in a buffer containing 25 mM HEPES (pH 7.5), 200 mM NaCl, 0.1% NP40, 10% glycerol and a protease inhibitor cocktail, and were completely lysed by passage through 22 G and 27 G needles (in that order) ten times. Cell extracts (500 to 2000 μg) were pre-cleared with protein G Sepharose beads (GE Healthcare), reacted with 4 μg of α-AhR, α-EGFP, α-FLAG or α-SARS, and then recovered with protein G beads. As an “input” sample, about 5 or 10% of each extract subjected to immunoprecipitation, was assessed simultaneously.
Immunohistochemical analysis of EGFP-positive cells
After perfusion fixation, organs were immersed in 0.1 M phosphate buffer (PB) (pH 7.4) supplemented with 4% paraformaldehyde at 4°C. On the following day, samples were serially immersed at 4°C in PB supplemented with 10% saccharose for 1 h, 20% saccharose until immersed completely, and then 30% saccharose overnight. Next, samples were embedded in Optimal Cutting Temperature compound (Sakura Finetek, Torrance, CA, USA) for cryosectioning. Using a cryostat (Leica Biosystems, Wetzlar, Germany), three slices (5 μm thick) were prepared from different sections of the fixed kidney: a first slice from the middle part of the kidney, a second section is from the part that contained mainly cortex with little amount of medulla, and the third section that is composed mainly of cortex. Samples were washed three times with 0.1 M phosphate-buffered saline (PBS) (10 min per wash), and incubated for 30 min at room temperature in Image-iT Fx signal enhancer (Invitrogen). After rinsing three times with 25 mM Tris-HCl (pH 7.5), 150 mM NaCl, and 0.05% Tween 20 (TBST) (10 min each), sections were then reacted with rabbit α-EGFP antibody (1:2000; Medical & Biological Laboratories) in TBST supplemented with 1% bovine serum albumin (BSA) at 4°C. On the following day, specimens were rinsed three times with TBST, and then incubated with Alexa Fluor 555-conjugated goat α-rabbit IgG antibody (1:5000; Invitrogen) for 2 h at room temperature. Nuclear DNA was stained with Hoechst 33258 at a final concentration of 0.36 μM. Fluorolabeled sections were examined under a fluorescence microscope (Olympus BX51; Olympus, Tokyo, Japan). Using the cellSens system (Olympus), total cell numbers in each section were first counted automatically. Next, numbers of EGFP-positive cells were counted manually. The frequency of EGFP-positive cells was calculated using the numbers of total and EGFP-positive cells. Three independent sections were prepared from a single specimen and subjected to analysis. The significance of the frequency of EGFP-positive cells was then evaluated statistically.
To identify RTECs positive for L1-RTP, immunohistochemistry was performed as described previously  using the following primary antibodies; α-GFP antibody (1:200 dilution) (Abcam, UK), α-AQP1 antibody (1:200 dilution) , and Alexa Fluor 546-phalloidin (1:400 dilution) (Invitrogen) .
L1-5′UTR methylation status
We performed sodium bisulfite treatment of genomic DNA using the EZ DNA Methylation Kit (Zymo Research, Irvine, CA, USA), according to the manufacturer’s instructions. One microlitre of the aliquot was used as the template for combined bisulfite restriction analysis (COBRA) . Primers used for amplification of the L1 transgene promoter region were as follows: forward 5′-GTAAGGGGTTAGGGAGTTTTT-3′ and reverse 5′-CCTTACAATTTAATCTCAAACTA-3′. The PCR reactions were performed in a volume of 20 μL containing 1 μL of bisulfite-treated genomic DNA, primers (0.3 μM each), and a 10 μL EpiTect MSP Kit (Qiagen, Hilden, Germany). The amplification conditions consisted of 40 cycles of 94°C for 15 sec, 50°C for 30 sec and 72°C for 30 sec. PCR products were digested using the restriction enzyme Taq I (New England Biolabs, Ipswich, MA, USA), which is specific for the methylated sequence, after sodium bisulfite treatment. Digested products were resolved by 3% agarose gel electrophoresis and stained with ethidium bromide.
Statistical significance was evaluated using the Mann-Whitney U-test. A P value < 0.05 was deemed to indicate statistical significance.
We are grateful to Drs. Elena T. Luning Prak (University of Pennsylvania Medical Center), Gilbert Nicolas (University of Michigan Medical School), and Gabriele Vielhaber (Symrise, Germany) for providing us with pEF06R, pCEP4/L1mneoI/ColE1, and MNF, respectively. We thank Ms. Rieko Yanobu-Takanashi for qPCR of the L1 transgenes in the L1-transgenic mice. Mr. Noriyuki Okudaira was an applicant supported by a Grant-in-Aid from the Tokyo Biochemical Research Foundation. This work was supported in part by Grants-in-Aid for Research from the National Center for Global Health and Medicine (22A-113), the Tokyo Biochemical Research Foundation and the Ministry of Health, Labor and Welfare of Japan (09156296).
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