Fig. 9
Effect of IN domain on cell proliferation and anchorage-independent growth. NIH 3T3 transfected by pRK5-HA-P50 or pRK5-HA-P50∆IN and then stably expressing HA-P50 or HA-P50∆IN were performed. (A) Cell proliferation was monitored daily using Presto Blue-coloration assay (Invitrogen) (CFM-cell free medium). (B) Effect of HA-P50 and HA-P50∆IN on anchorage-independent growth was monitored using soft agar assay. Absorbance-based quantitative analysis of the different cell lines grown in the agar matrix at day 14 was performed with Presto Blue-coloration assay (Invitrogen). Results are given in Relative Fluorescence Units (RFU). Experiments were performed in triplicates and error bars indicate SEM. The significance of differences was assessed using a one-way ANOVA test (****p ≤ 0.0001 and *p ≤ 0.1, ns, not significant)