Fig. 8

Effects of P50 domains on MLV promotor activity. (A) Schematic of the different HA-P50 constructs. The amino acid positions of P50 domains are given in bold. The molecular weights are in brackets. (B) Expression of the different HA-P50 constructs was checked by WB analysis with anti-HA antibody. The stars show the localization of N-term CA and C-term IN domain. Experiments were performed in triplicates. (C) MLV promotor expression. NIH 3T3 co-transfected with bidir MLV-LTR and pRK5-HA-P50 or empty pRK5-HA constructs. Luciferase activity was analyzed 48 h post-transfection and values obtained with only bidir MLV-LTR plasmid was referred as 100%. Experiments were performed in triplicates and the significance of differences was assessed using an unpaired Student’s t-test (ns: non significative, ****P ≤ 0.0001, ***P ≤ 0.001, **P ≤ 0.01 and *P ≤ 0.1)