Fig. 6

Subcellular localization of P50. (A) NIH 3T3 cells were transiently transfected with pSD’GFP (see Methods). Two-days post-transfection, cells were treated or not with 40 nM of LMB for 4 h and subcellular localization analysis was performed with confocal microscopy. Nuclei were stained with DAPI (blue) and P50-GFP appeared in green (GFP channel). Scale bar is 10 μm. (B) Total cell extracts, cytoplasmic and nuclear fractions of cells transfected with p57SD’/GFP or empty peGFP plasmid, were analyzed by WB using antibodies against GFP, GAPDH and Histone 3. The stars indicate the position of P50-GFP.