Fig. 4

Effects of P50 on anchorage-independent growth. NIH 3T3 cells were stably transfected with SD’-WT, SD’-STOP or Gag or chronically infected with F57 or F1. Effect of P50 on anchorage-independent growth was monitored using soft agar assay. (A) Micrographs showing the colonies formed in the agar matrix at day 14. The scale bar corresponds to 1 mm (B) Absorbance-based quantitative analysis of the different cell lines. Experiments were performed in triplicates and error bars indicate SEM. The significance of differences was assessed using a one-way ANOVA test (****p ≤ 0.0001 and *p ≤ 0.1, ns, not significant)