Fig. 3

Effect of P50 on cell proliferation. (A) Schematic representation of SD’ RNA WT or with STOP mutation (in red) inhibiting the P50 expression. NIH 3T3 cells were stably transfected with either SD’ WT, SD’ STOP or Gag 2LTR or chronically infected with F57 or F1 viruses. (B) The expression of P50 was monitored by WB, using the anti-All-Friend antibody, in F57 and F1 infected cells and in SD’ WT- and SD’ STOP- stably expressing cells. SD” corresponds to the product (42KDa) of an cryptic splicing that is artificially woken up when SD’ alternative splicing was inactive [11]. Effect of expression of P50 or SD’RNA (C), MLV Gag (D) or F57 or F1 viruses (E) on cellular proliferation was monitored daily using CCK8-coloration assay (Dojindo) (CFM-cell free medium). Experiments were performed in triplicate and the significance of differences was assessed using a one-way ANOVA test (****p ≤ 0.0001 and *p ≤ 0.1, ns, not significant)