Table 3 Oligonucleotide primers used for qRT-PCR
From: Analysis of PERV-C superinfection resistance using HA-tagged viruses
Primer | Target gene | Sequence 5′-3′ | Accession No | Nucleotide pos | Amplicon |
|---|---|---|---|---|---|
F | PERV pol | TCTCCCCAAGTAAAGCCTGAT | AJ133816.1 | 3119–3139 | 227 nt |
R | PERV pol | ACTAGGATGCCCTGTTGGATTA | AJ133816.1 | c3345–3324 | |
EnvC-for2 | PERV envC | GTGCTCTCCTTCAGACCTAGATTAC | AM229312.2 | 9383–9407 | 184 nt |
EnvC-R3_AWG | PERV envC | AGCCATTGGAGGCTCCAGCTGG | AM229312.2 | c9566–9545 | |
RV_EnvC-HA | PERV envC-HA | CGCATAATCCGGCACATCATACGGATA | Â | Â | Â |
EnvC-for3 | PERV envC-HA | CGTTAAGCCGGCGCCACC | KY352351.2 | 6388–6405 | 146 nt |
EnvC-for4 | PERV envC-HA | GTTGGAGAAGCGTCGAAGGGAAAAGG | KY352351.2 | 8042–8067 | 277 nt |