Table 1 Oligonucleotide primers used for cloning of PERV-C(5683)-HA plasmids
From: Analysis of PERV-C superinfection resistance using HA-tagged viruses
Primer | Target gene | Sequence 5′-3′* | Accession No | Nucleotide pos |
|---|---|---|---|---|
Env_SP-HA_F | PERV envC | GCCGGATTATGCGATGCGCATA GGAGACAGCCTG | KY352351.2 | 6507–6524 |
cEnv_SP-HA_R | PERV envC | ACATCATACGGATAACCATTAG TCTGAGAGGTTATTGACAGAG | KY352351.2 | 6484–6506 |
Env_HA-VRA_F | PERV envC | GCCGGATTATGCGGATCTATACG TTTGCCTCAGATCAGTTATTCC | KY352351.2 | 6633–6664 |
cEnv_HA-VRA_R | PERV envC | ACATCATACGGATAAGGCCACC AGGTTCCTAAAGG | KY352351.2 | 6612–6632 |
Env_RPep-HA_F | PERV envC | GCCGGATTATGCGTAGCTCTACC AGTTCTAAGATTAGAAC | KY352351.2 | 8292–8318 |
cEnv_RPep-HA_R | PERV envC | ACATCATACGGATAGCGGCCAGC TTCCCTGCT | KY352351.2 | 8274–8291 |