Fig. 7

Quantification of vRNA and RT activity after challenging of ST-IOWA/PERV-C(5683) with PERV-C(5683)-HA viruses. Expression of PERV-C env vRNA was measured by qRT-PCR using A envC and B, C envC-HA specific primers. D Enzyme activity was monitored by RT assay. Native ST-IOWA cells were used as negative controls (ctr−), ST-IOWA cells infected with PERV-C(5683)-HA served as positive controls to confirm virus functionality. A Expression of envC vRNA was measured for all cells except for ST-IOWA (ctr−) starting from day 7 on. B, C envC-HA vRNA was detected for ST-IOWA cells infected with SP-HA or RPep-HA containing cell culture SNs but not for PERV-C(5683) positive cells challenged with PERV-C(5683)-HA. Significant means of < 0.05 according to Welch’s t test are indicated by (*). Analysis were performed in triplicates