Fig. 2

Quantification of vRNA and RT activity in ST-IOWA cells transfected with HA-tagged PERV-C(5683). Cells were monitored for 56 days by collecting cell culture SNs weekly. Expression of PERV-C(5683) A env and B pol vRNA for SP-HA and RPep-HA showed equal levels compared to the positive control PERV-C(5683) (ctr+). HA-VRA transfected cells revealed only basal expression comparable to the PERV-C negative ST-IOWA cells (ctr−). C Consistent to the presence of vRNA PERV-C(5683), SP-HA and RPep-HA became RT positive within two weeks after transfection. HA-VRA remained negative for RT activity. Significant means of < 0.05 according to Welch’s t-test are indicated by (*). Analysis were performed in triplicates