Fig. 1

Structure and design of PERV-C(5683)-HA constructs. The HA-tag was inserted into the env gene of PERV-C(5683) (KY352351.2) by site directed mutagenesis. A Regular structure of PERV-C(5683) env. PERV-C(5683) molecular clones carrying the HA-tag B C-terminal to the SP at amino acid (AA) 44–52 (SP-HA), C N-terminal to the VRA at AA 86–94 (HA-VRA) or D C-terminal to the RPep at AA 639–647 (RPep-HA). The integration sites of HA were chosen on the basis of functional studies [9, 25, 56, 57] and secondary structure analyses using the open source tool PSIPRED [55]