Fig. 4
From: Primate TRIM34 is a broadly-acting, TRIM5-dependent lentiviral restriction factor
TRIM34 SPRY domain is necessary for restriction of SIVAGM-SAB. a. Schematic of TRIM34 RBCC-TRIM5 SPRY chimera. b. THP-1 TRIM34 clonal KO cells were generated by electroporation of multiplexed sgRNA against TRIM34 and single cell sorting. Cells were transduced with doxycycline-inducible HA-tagged human TRIM34, TRIM5α, or TRIM34-TRIM5α chimeras. Expression was induced in the presence of 125 ng/mL doxycycline, and expression levels were visualized by immunoblotting 72 h post-induction. c. THP-1 TRIM34 clonal KO cells expressing doxycycline-inducible human TRIM34, TRIM5α, or TRIM34 RBCC-TRIM5 SPRY were infected with SIVAGM-SAB CA or SIVAGM-TAN-luc particles 1 day post-induction. Level of infection was quantified 2 dpi by flow cytometry or luminometry, respectively. Relative infectivity in induced cells (white bars, circles) is normalized to average infectivity in uninduced control cells (grey bars, triangles). Fold inhibition is indicated where applicable. Infection of each cell line with each virus was performed a total of 6 times across 2 different occasions. Combined data are represented as mean +/- s.d., where each point represents a unique infection. One-sided p values were calculated by Welch’s t-test. ns nonsignificant, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001