Skip to main content
Fig. 2 | Retrovirology

Fig. 2

From: Transmitted/founder SHIV.D replicates in the brain, causes neuropathogenesis, and persists on combination antiretroviral therapy in rhesus macaques

Fig. 2

Quantitative viral RNA and DNA in situ hybridization in TF SHIV.D RM brain tissue. RNAscope (left) and DNAscope (right) in situ hybridization (ISH) of replicating viral RNA and proviral DNA in brain tissue of progression, off-ART, and ART suppressed RM (A). Representative images from occipital, temporal, and frontal lobes are shown at 10X (RNA) and 20X (DNA) magnification. Inserts = 40X magnification. Scale bar = 200 μm. The area of SHIV.D RNA positive signal (µm2) or the number of nuclei containing proviral DNA/mm2 were quantified using Keyence BZ-X700 Microscope and accompanying Batch Analysis Software to determine the average area of positive signal (RNA) or the mean number of SHIV.D DNA-containing nuclei (DNA) from 10 nonoverlapping 20X images per brain section of each RM (B). 20X frame area = 393,880 μm². Box and whisker plots are presented as mean values of counts ± quartiles, with whiskers representing the range

Back to article page

Retrovirology

ISSN: 1742-4690

Contact us