Fig. 3

Catalytic active TOP1 restores the repression of HIV-1 LTR promoter in Top1 depleted J-Lat A1 cells. WT or two CRISPR TOP1 clones of J-Lat A1 cells were transduced by a pTRIP vector expressing WT or Y723F TOP1 or no protein (nc). A Seven days post-transduction, the presence of recombinant and endogenous TOP1 proteins was evaluated by SDS-PAGE and western blot directed against TOP1. B Concomitantly, the % of GFP-positive cells was measured by FACS and normalized to the % measured in WT cells, transfected by an empty vector (n = 4 for empty vector and n = 3 for expressed protein conditions)