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Fig. 1 | Retrovirology

Fig. 1

From: DNA topoisomerase 1 represses HIV-1 promoter activity through its interaction with a guanine quadruplex present in the LTR sequence

Fig. 1

Top1 shRNA silencing induces a reactivation of HIV-1 LTR promoter activity in HeLa-LTR luciferase cells. HeLa LTR luciferase cells were transduced with pLKO.1 vector expressing shRNAs directed against Top1, Top2A or Top2B genes or a scrambled shRNA sequence (sh-), at ratios of 100–300 µL vector/2. 106 cells. A The amount of TOP1, TOP2A and TOP2B proteins was evaluated 7 days post-transduction by SDS-PAGE and Western-Blot of cellular extracts. The Luciferase activity (B) or the levels of mRNA coding for the luciferase gene (C) were also quantified 7 days post-transduction (n = 5) at the highest transduction ratio

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Retrovirology

ISSN: 1742-4690

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