Table 2 Heterogeneous-ligand modelinga
From: Conformational antigenic heterogeneity as a cause of the persistent fraction in HIV-1 neutralization
SOSIP.664 trimer | Fab | Affinity purification | kon1b (1/Ms) | koff1b (1/s) | kon2b (1/Ms) | koff2b (1/s) | KD1b (nM) | KD2b (nM) | Sm1b | Sm2b | Sm1b + Sm2b |
|---|---|---|---|---|---|---|---|---|---|---|---|
BG505 | PGT145 | 2G12 (n = 3) | 2.9 × 104 ± 7.5 × 102 |  < 10–5 | 5.0 × 103 ± 7.0 × 102 | 2.0 × 10–3 ±  .1 × 10–5 |  < 0.5 | 4.1 × 102  ± 64 | 0.46 ± 1.6 × 10–2 | 0.46  ± 2.4 × 10–2 | 0.92  ± 3.8 × 10–2 |
PGT151 | 2G12 (n = 2) | 1.4 × 105 ± 1.3 × 103 |  < 10–5 | 2.8 × 104 ± 1.0 × 103 | 7.6 × 10–4 ±  .5 × 10–5 |  < 0.1 | 27 ± 1.9 | 1.2 ± 1.9 × 10–2 | 0.70  ± 1.1 × 10–2 | 1.9 ± 7.7 × 10–3 | |
B41 | PGT145 | 2G12 (n = 3) | 5.2 × 104 ± 1.4 × 104 | 1.9 × 10–4 ± 3.6 × 10–5 | 1.6 × 104 ± 1.5 × 103 | 1.1 × 10–4 ±  .7 × 10–5 | 3.7 ± 0.23 | 6.6 ± 0.80 | 4.4 × 10–2 ±  .4 × 10–2 | 0.59 ± 3.6 × 10–2 | 0.63 ± 1.8 × 10–2 |
PGT145 (n = 3) | 3.5 × 104 ± 1.4 × 104 |  < 10–5 | 4.5 × 104 ±  1.1 × 104 |  < 10–5 |  < 0.5 |  < 0.5 | 0.13 ± 0.13 | 0.74 ± 0.16 | 0.87 ± 5.1 × 10–2 | ||
PGT151 | 2G12 (n = 2) | 3.1 × 104 ± 1.9 × 103 | 8.5 × 10–4 ±  2.5 × 10–4 | 2.5 × 104 ±  5.5 × 103 | 7.8 × 10–4 ±  .8 × 10–5 | 28 ± 9.8 | 34 ± 12 | 0.45 ± 2.9 × 10–2 | 4.1 × 10–3  ± 4.0 × 10–3 | 0.45 ± 2.5 × 10–2 |