Table 1 Langmuir modelinga
From: Conformational antigenic heterogeneity as a cause of the persistent fraction in HIV-1 neutralization
SOSIP.664 trimer | Fab | Affinity purification | konb (1/Ms) | koffb (1/s) | KDb (nM) | Smb |
|---|---|---|---|---|---|---|
BG505 | PGT145 | 2G12 (n = 3) | 1.8 × 104 ± 3.7 × 102 | 5.1 × 10–4 ± 2.0 × 10–6 | 29 ± 0.69 | 0.74 ± 6.8 × 10–3 |
PGT151 | 2G12 (n = 2) | 8.7 × 104 ± 3.5 × 102 | 1.8 × 10–4 ± 5.0 × 10–7 | 2.0 ± 1.3 × 10–2 | 1.9 ± 7.5 × 10–3 | |
B41 | PGT145 | 2G12 (n = 3) | 2.0 × 104 ± 8.8 × 102 | 1.2 × 10–4 ± 1.5 × 10–5 | 6.2 ± 0.28 | 0.63 ± 1.6 × 10–2 |
PGT145 (n = 3) | 3.2 × 104 ± 2.3 × 103 |  < 10–5 |  < 0.5 | 0.87 ± 4.8 × 10–2 | ||
PGT151 | 2G12 (n = 3) | 4.0 × 104 ± 4.1 × 103 | 3.1 × 10–3 ± 2.4 × 10–4 | 77 ± 5.0 | 0.45 ± 1.5 × 10–2 | |
PGT145 (n = 2) | Minimal binding: < 10 RU | |||||